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N (NC_004148) Virus Tagged ORF Clone
CAT＃: VC101753
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for nucleoprotein [Human metapneumovirus], codon optimized for human cell expression, YP_012605
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CNY 4,660.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC101753 represents NCBI reference of YP_012605 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCACTGCAGGGAATCCACTTGTCTGACCTTAGTTACAAGCATGCGATTCTGAAGGAATCACAGTACA CTATCAAGAGAGACGTGGGAACGACGACAGCTGTGACACCTAGCTCCTTGCAACAGGAAATCACCCTTTT GTGCGGGGAAATTCTGTACGCGAAACATGCAGACTATAAGTACGCGGCCGAAATCGGTATTCAGTATATA TCCACCGCACTCGGATCCGAGCGCGTGCAGCAGATACTCCGAAACTCTGGCTCAGAGGTGCAGGTGGTAC TTACTAGGACCTATTCTCTGGGAAAGGTGAAGAACAACAAAGGCGAGGATCTCCAGATGCTCGACATTCA TGGCGTTGAAAAAAGTTGGGTGGAGGAAATCGATAAGGAGGCGAGGAAGACAATGGCCACTCTGCTGAAA GAGTCTAGTGGGAACATCCCCCAGAATCAGCGCCCCTCCGCACCCGACACACCAATCATCCTCCTCTGCG TGGGCGCCCTGATTTTTACGAAACTCGCCTCCACTATAGAAGTGGGGCTCGAAACTACGGTACGGAGAGC CAATAGGGTGCTGTCCGATGCCCTGAAACGATACCCAAGAATGGACATACCTAAAATTGCCAGGAGCTTC TACGATCTGTTCGAACAGAAAGTGTATTACCGATCATTGTTTATCGAATACGGGAAGGCCCTGGGTTCCA GTAGTACAGGTTCCAAGGCGGAGTCCCTGTTCGTTAATATCTTCATGCAGGCTTACGGGGCTGGGCAGAC AATGCTCCGGTGGGGAGTGATCGCCAGAAGTAGTAATAACATCATGTTGGGGCATGTGAGCGTGCAGGCG GAGCTTAAACAAGTCACTGAAGTGTATGATTTGGTGCGGGAGATGGGGCCAGAGTCAGGATTGTTGCACC TGAGGCAGTCCCCTAAGGCTGGTCTGCTCTCCCTCGCTAATTGCCCCAATTTCGCCTCAGTCGTTCTGGG CAATGCCTCCGGTCTGGGCATCATCGGAATGTATAGGGGGCGGGTGCCGAATACGGAACTTTTTAGCGCT GCGGAGAGCTATGCGAAAAGCCTGAAAGAGTCTAACAAGATCAATTTTAGTTCTCTGGGGCTCACTGATG AAGAGAAAGAGGCCGCCGAGCATTTTTTGAACGTTAGCGATGACAGTCAGAATGATTATGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC101753 representing YP_012605
Red=Cloning sites Green=Tags MSLQGIHLSDLSYKHAILKESQYTIKRDVGTTTAVTPSSLQQEITLLCGEILYAKHADYKYAAEIGIQYI STALGSERVQQILRNSGSEVQVVLTRTYSLGKVKNNKGEDLQMLDIHGVEKSWVEEIDKEARKTMATLLK ESSGNIPQNQRPSAPDTPIILLCVGALIFTKLASTIEVGLETTVRRANRVLSDALKRYPRMDIPKIARSF YDLFEQKVYYRSLFIEYGKALGSSSTGSKAESLFVNIFMQAYGAGQTMLRWGVIARSSNNIMLGHVSVQA ELKQVTEVYDLVREMGPESGLLHLRQSPKAGLLSLANCPNFASVVLGNASGLGIIGMYRGRVPNTELFSA AESYAKSLKESNKINFSSLGLTDEEKEAAEHFLNVSDDSQNDYE TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_004148 |
| ORF Size | 1182 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NC_004148.2, YP_012605 |
| RefSeq ORF | 1182 bp |
| Locus ID | 2799936 |
| MW | 43.5 kDa |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC101754 | Myc-DDK-tagged ORF clone of viral ORF for phosphoprotein [Human metapneumovirus], codon optimized for human cell expression, YP_012606 |
CNY 3,800.00 |
|
| VC101755 | Myc-DDK-tagged ORF clone of viral ORF for matrix protein [Human metapneumovirus], codon optimized for human cell expression, YP_012607 |
CNY 3,800.00 |
|
| VC101756 | Myc-DDK-tagged ORF clone of viral ORF for fusion glycoprotein precursor [Human metapneumovirus], codon optimized for human cell expression, YP_012608 |
CNY 6,560.00 |
|
| VC101757 | Myc-DDK-tagged ORF clone of viral ORF for matrix protein M2-1 [Human metapneumovirus], codon optimized for human cell expression, YP_012609 |
CNY 3,800.00 |
|
| VC101758 | Myc-DDK-tagged ORF clone of viral ORF for matrix protein M2-2 [Human metapneumovirus], codon optimized for human cell expression, YP_012610 |
CNY 3,800.00 |
|
| VC101759 | Myc-DDK-tagged ORF clone of viral ORF for small hydrophobic protein [Human metapneumovirus], codon optimized for human cell expression, YP_012611 |
CNY 3,800.00 |
|
| VC101760 | Myc-DDK-tagged ORF clone of viral ORF for attachment glycoprotein G [Human metapneumovirus], codon optimized for human cell expression, YP_012612 |
CNY 3,800.00 |
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