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proteinase 3C (NC_001430) Virus Tagged ORF Clone
CAT＃: VC100777
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for proteinase 3C [Human enterovirus D], codon optimized for human cell expression, NP_740421
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CNY 3,800.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
| Product Data | |
| Type | Virus Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>The Viral ORF clone VC100777 represents NCBI reference of NP_740421 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCCTTCCCTGGATTTTGCCCAGGCTATAATGAGGAAAAATACCGTCATTGCTAGGACCTCAAAGG GCGAATTCACTATGCTGGGAATTTATGACCGGATCGCCGTGGTTCCGACCCACGCCTCAGTCGAGGAGGA GATCTACATCAACGACGTACCCGTTAAAGTAAAAGACGCCTACGCTCTCAGGGACATAAATGACGTTAAC CTCGAAATCACAGTAGTGGAACTGGATAGGAACGAAAAGTTTCGCGATATCCGCGGCTTCCTGCCTAAGT ACGAAGACGACTACAACGATGCAATACTTAGCGTGAACACCTCCAAGTTCCCTAATATGTATATCCCCGT GGGGCAGACACTGAATTACGGCTTTCTGAATCTGGGTGGGACTCCGACCCACAGAATCCTCATGTATAAC TTTCCCACACGGGCTGGACAGTGTGGAGGAGTAGTTACCACTACAGGCAAGGTCATCGGAATCCATGTGG GAGGCAATGGTGCTCAGGGCTTCGCAGCAATGCTGCTCCAGAATTACTTCACTGAGAAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100777 representing NP_740421
Red=Cloning sites Green=Tags MGPSLDFAQAIMRKNTVIARTSKGEFTMLGIYDRIAVVPTHASVEEEIYINDVPVKVKDAYALRDINDVN LEITVVELDRNEKFRDIRGFLPKYEDDYNDAILSVNTSKFPNMYIPVGQTLNYGFLNLGGTPTHRILMYN FPTRAGQCGGVVTTTGKVIGIHVGGNGAQGFAAMLLQNYFTEKQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
| ACCN | NC_001430 |
| ORF Size | 552 bp |
| OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NC_001430.1, NP_740421 |
| RefSeq ORF | 552 bp |
| MW | 20.4 kDa |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| VC100764 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1A [Human enterovirus D], codon optimized for human cell expression, NP_740412 |
CNY 3,800.00 |
|
| VC100765 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1B [Human enterovirus D], codon optimized for human cell expression, NP_740413 |
CNY 3,800.00 |
|
| VC100766 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1C version 3 [Human enterovirus D], codon optimized for human cell expression, NP_740738 |
CNY 3,800.00 |
|
| VC100767 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1C version 1 [Human enterovirus D], codon optimized for human cell expression, NP_740739 |
CNY 3,800.00 |
|
| VC100768 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1C version 2 [Human enterovirus D], codon optimized for human cell expression, NP_740740 |
CNY 3,800.00 |
|
| VC100769 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1D version 2 [Human enterovirus D], codon optimized for human cell expression, NP_740741 |
CNY 3,800.00 |
|
| VC100770 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1D version 1 [Human enterovirus D], codon optimized for human cell expression, NP_740742 |
CNY 3,800.00 |
|
| VC100771 | Myc-DDK-tagged ORF clone of viral ORF for capsid protein 1D version 3 [Human enterovirus D], codon optimized for human cell expression, NP_740743 |
CNY 3,800.00 |
|
| VC100772 | Myc-DDK-tagged ORF clone of viral ORF for proteinase 2A [Human enterovirus D], codon optimized for human cell expression, NP_740416 |
CNY 3,800.00 |
|
| VC100773 | Myc-DDK-tagged ORF clone of viral ORF for protein 2B [Human enterovirus D], codon optimized for human cell expression, NP_740417 |
CNY 3,800.00 |
|
| VC100774 | Myc-DDK-tagged ORF clone of viral ORF for NTPase 2C [Human enterovirus D], codon optimized for human cell expression, NP_740744 |
CNY 3,800.00 |
|
| VC100775 | Myc-DDK-tagged ORF clone of viral ORF for protein 3A [Human enterovirus D], codon optimized for human cell expression, NP_740419 |
CNY 3,800.00 |
|
| VC100776 | Myc-DDK-tagged ORF clone of viral ORF for VPg 3B [Human enterovirus D], codon optimized for human cell expression, NP_740745 |
CNY 3,800.00 |
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