2A (NC_009887) Virus Tagged ORF Clone

CAT＃: VC100672

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for 2A [Human enterovirus 100], codon optimized for human cell expression, YP_001497167

Datasheet

SDS

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CNY 3,800.00

货期*

4周

规格

Product images

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Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100672 represents NCBI reference of YP_001497167 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCGCCTTCGGGCAGCAGAGCGGGGCAGTTTATGTTGGGAGCTACCGCATTGTGAATAGGCACCTCG CAACCTACACAGATTGGCAGAACTGTATCTGGGAGGACTATAATAGGGATCTGCTGGTCTCCACCACCAC AGCTCAGGGCTGTGACATTATTGCCCGCTGCCAGTGCACCACCGGAATTTACTTCTGTCAGAGCAAAAAT AAACACTATCCAGTCTCTTTTGAAGGCCCCGGCCTTGTAGAGGTCCAGGAATCCGAGTATTACCCTAAAC GCTACCAGAGTCATGTATTGTTGGCCGCCGGATTCAGTGAGCCAGGAGACTGCGGGGGCATCCTGAGGTG CGAGCACGGCGTTATCGGCGTGGTTACGATGGGAGGCGAGGGAGTAGTGGGCTTCGCCGATGTCCGGGAC CTCCTCTGGTTGGAGGACGATGCGATGGAGCAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100672 representing YP_001497167 Red=Cloning sites Green=Tags MGAFGQQSGAVYVGSYRIVNRHLATYTDWQNCIWEDYNRDLLVSTTTAQGCDIIARCQCTTGIYFCQSKN KHYPVSFEGPGLVEVQESEYYPKRYQSHVLLAAGFSEPGDCGGILRCEHGVIGVVTMGGEGVVGFADVRD LLWLEDDAMEQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	NC_009887
ORF Size	453 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_009887.1, YP_001497167
RefSeq ORF	453 bp
MW	16.7 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC100668	Myc-DDK-tagged ORF clone of viral ORF for 1A [Human enterovirus 100], codon optimized for human cell expression, YP_001497163	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100669	Myc-DDK-tagged ORF clone of viral ORF for 1B [Human enterovirus 100], codon optimized for human cell expression, YP_001497164	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100670	Myc-DDK-tagged ORF clone of viral ORF for 1C [Human enterovirus 100], codon optimized for human cell expression, YP_001497165	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100671	Myc-DDK-tagged ORF clone of viral ORF for 1D [Human enterovirus 100], codon optimized for human cell expression, YP_001497166	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100673	Myc-DDK-tagged ORF clone of viral ORF for 2B [Human enterovirus 100], codon optimized for human cell expression, YP_001497168	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100674	Myc-DDK-tagged ORF clone of viral ORF for 2C [Human enterovirus 100], codon optimized for human cell expression, YP_001497169	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100675	Myc-DDK-tagged ORF clone of viral ORF for 3A [Human enterovirus 100], codon optimized for human cell expression, YP_001497170	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100676	Myc-DDK-tagged ORF clone of viral ORF for 3B [Human enterovirus 100], codon optimized for human cell expression, YP_001497171	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100677	Myc-DDK-tagged ORF clone of viral ORF for 3C [Human enterovirus 100], codon optimized for human cell expression, YP_001497172	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

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