VP2 (NC_012798) Virus Tagged ORF Clone

CAT#: VC100645

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for VP2 [Human cosavirus E], codon optimized for human cell expression, YP_002956078



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

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经常一起买 (4)
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100645 represents NCBI reference of YP_002956078 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCCCCTCTGCCGAGAACTCTACCAACATGGGCGACCGCATTCTGACCCTCCGGGAAGGAAATACAG
CGATTAGCTCTCAGGCTACAGAGGGTATTTTGCACGGGTACAATAAAGAAACGGACACTAGCCAGCCGAC
CTCCTGTGGAGATAGGGCCTCTAAAGCCACCCCCGCCACCCAACGATCCTTTATCATTCCCTTGAAGGAC
TGGACCAGTTCCCAGAATAGCTACTCTGCCCTCGTGTACAGAACGAGCCACATGTCTAAGGATGCCGTCG
GGAACTTGTTCTCTAAAAACATGAACACCCACGCTTATACCAAGACTGGGTGGACAGCAACACTTCAAAT
CAATACTTCTCCCTTTCATAGCGGGTTGATCGGCCTTTTTATGGTACCCGAATTCGTGAGAAAAATTTCT
GATGACTTGGAATGGATTGATCTCGTCGCAGCAGGTTTTAACACGACGCTCTTTAAGGACTCAAATACAT
ATAACCAGGACAGCGCTCTGGTCGGAAAAATCGATGACAAAAACACCTTTGACCTCGCGTCCACCACGCC
CGAACAGTTCTTTCTGTATCCTCACCAACTTATTAATCCAAAAGAGACTACCATGGCTACTGTGTCAGTG
CCTTACGTAAATGTCGCACCATCTTCCGATCCTTCTGTCCACAACATATGGACAGTCGTCGTCATGGTGC
TGTCCCCCCTTCAGATTGGGAGCGGGGCTTCCCCCAACGTGCAGATGACACTCACTCTGACCCCCACAGG
AAGCGTGTTTAATGGACTTCGGCACCCTAAACCAGCCGCTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100645 representing YP_002956078
Red=Cloning sites Green=Tags

MTPSAENSTNMGDRILTLREGNTAISSQATEGILHGYNKETDTSQPTSCGDRASKATPATQRSFIIPLKD
WTSSQNSYSALVYRTSHMSKDAVGNLFSKNMNTHAYTKTGWTATLQINTSPFHSGLIGLFMVPEFVRKIS
DDLEWIDLVAAGFNTTLFKDSNTYNQDSALVGKIDDKNTFDLASTTPEQFFLYPHQLINPKETTMATVSV
PYVNVAPSSDPSVHNIWTVVVMVLSPLQIGSGASPNVQMTLTLTPTGSVFNGLRHPKPAAQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_012798
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_012798.1, YP_002956078
RefSeq ORF 813 bp
MW 29.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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