HCoV-229E S Gene Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100563 represents NCBI reference of NP_073551 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCGTTCTCCTGGTGGCCTATGCCCTGTTGCACATTGCAGGATGCCAGACAACCAACGGACTGAACA CCAGTTACTCTGTGTGTAACGGCTGTGTGGGTTACTCCGAGAACGTGTTCGCAGTGGAAAGTGGCGGTTA TATACCTTCTGACTTTGCATTTAATAACTGGTTTCTGCTCACCAATACTTCAAGCGTGGTAGATGGGGTC GTAAGGTCCTTCCAACCATTGCTCCTTAACTGTTTGTGGAGCGTGAGCGGGCTTAGATTCACCACAGGCT TTGTATACTTCAATGGGACTGGCCGGGGTGATTGCAAAGGGTTCTCAAGCGATGTGCTGTCCGATGTAAT TAGGTATAATCTCAATTTCGAAGAAAATCTGCGAAGAGGAACAATACTCTTTAAGACTAGCTACGGGGTG GTTGTCTTCTACTGCACCAATAACACTCTCGTTTCAGGGGATGCGCATATTCCATTTGGCACAGTGCTTG GGAATTTTTATTGCTTCGTGAATACAACGATCGGGAACGAGACCACAAGTGCCTTCGTTGGTGCACTTCC AAAGACGGTGCGAGAGTTTGTTATCTCCCGAACTGGGCACTTTTACATTAACGGTTACAGGTACTTTACC CTGGGAAACGTCGAGGCCGTAAATTTTAACGTCACGACTGCTGAGACAACCGACTTTTGTACCGTGGCCC TGGCTTCCTACGCCGATGTATTGGTGAACGTGAGCCAGACCTCTATCGCTAACATCATCTACTGCAACTC CGTAATCAACAGACTCCGGTGTGACCAGTTGTCTTTCGACGTCCCTGACGGCTTCTACTCCACGAGTCCT ATTCAGTCAGTCGAATTGCCGGTGAGTATTGTTAGCCTGCCCGTCTACCACAAACACACATTCATTGTGC TGTACGTAGATTTTAAACCTCAGTCTGGCGGAGGAAAGTGCTTTAATTGCTACCCTGCAGGTGTCAATAT TACCCTTGCTAACTTCAATGAAACCAAAGGTCCTTTGTGTGTCGACACATCCCATTTCACTACAAAGTAT GTGGCGGTCTATGCCAACGTGGGGAGGTGGTCAGCCTCAATAAATACTGGCAATTGCCCTTTTAGTTTTG GAAAGGTCAACAATTTCGTCAAATTTGGGAGCGTCTGCTTTAGCCTCAAAGACATTCCCGGTGGTTGCGC CATGCCGATTGTTGCTAATTGGGCCTATAGCAAATACTATACAATAGGAAGCTTGTACGTCAGCTGGAGT GATGGCGATGGGATTACGGGTGTCCCGCAGCCCGTGGAGGGGGTGTCTTCATTTATGAACGTGACCCTTG ACAAGTGCACCAAGTACAATATCTACGATGTGTCCGGGGTTGGCGTTATAAGAGTCTCCAACGATACATT CCTCAACGGTATCACCTACACCAGTACGTCTGGAAATCTTCTCGGCTTTAAGGACGTGACTAAGGGCACA ATATACTCTATCACCCCATGCAATCCCCCCGACCAACTTGTTGTCTACCAGCAGGCCGTGGTAGGCGCTA TGCTCTCTGAAAACTTTACGTCATACGGGTTCTCCAATGTCGTAGAACTGCCAAAATTCTTTTATGCCTC TAACGGGACTTACAACTGTACCGACGCAGTGTTGACCTACAGCAGCTTCGGTGTGTGTGCCGACGGTTCC ATCATTGCCGTGCAGCCTCGAAATGTGAGCTACGATAGCGTTAGCGCGATCGTAACGGCCAACTTGAGCA TCCCTTCAAACTGGACCACTTCAGTTCAGGTGGAATATTTGCAGATTACCTCTACACCTATTGTAGTTGA TTGCTCCACATATGTGTGTAACGGAAATGTGCGGTGCGTGGAACTCCTCAAGCAATATACAAGCGCCTGT AAGACAATCGAGGACGCCCTGCGGAATAGCGCAAGGCTGGAGTCTGCCGATGTCAGTGAAATGCTTACCT TCGATAAGAAGGCTTTCACGTTGGCAAATGTATCCTCATTTGGCGATTATAATCTCTCCAGTGTCATTCC TAGTCTCCCCACAAGTGGGAGTAGAGTGGCCGGACGAAGCGCCATTGAAGACATCCTCTTCTCCAAGCTC GTCACAAGTGGTCTGGGCACTGTGGACGCAGATTATAAAAAGTGTACTAAAGGCCTTAGCATCGCTGATC TCGCATGTGCACAGTACTACAACGGGATTATGGTGCTGCCTGGCGTGGCCGATGCTGAACGCATGGCTAT GTATACCGGATCCCTCATAGGTGGCATAGCTCTTGGGGGACTTACCAGTGCGGTGAGCATACCATTCTCT CTCGCTATCCAGGCACGGCTGAACTATGTTGCATTGCAGACAGATGTGCTGCAGGAAAACCAGAAGATCC TGGCCGCATCTTTCAACAAGGCAATGACAAACATCGTGGACGCCTTCACTGGCGTTAATGACGCCATCAC ACAGACAAGCCAAGCACTCCAGACCGTGGCCACCGCCCTGAACAAGATTCAGGATGTGGTGAACCAGCAA GGCAACTCCCTGAATCACCTCACCAGTCAGTTGCGCCAAAATTTTCAGGCGATCTCTTCATCCATTCAGG CAATATACGACCGCCTGGATACCATACAGGCGGATCAGCAAGTTGACCGCCTGATAACCGGACGACTCGC CGCCCTGAATGTGTTTGTTAGTCATACTCTGACAAAATATACCGAAGTTCGAGCTTCCAGGCAACTCGCA CAGCAGAAAGTGAACGAGTGCGTCAAGAGTCAGAGCAAGAGGTACGGCTTTTGTGGTAATGGCACCCACA TCTTCTCCATCGTGAACGCTGCACCTGAGGGATTGGTGTTTCTGCATACAGTACTTCTGCCCACTCAATA TAAAGATGTTGAGGCCTGGTCTGGGCTTTGCGTAGATGGAACCAACGGGTACGTGCTGAGACAACCCAAC CTCGCTCTCTATAAGGAAGGCAATTATTATCGGATAACCTCCCGGATTATGTTCGAGCCTCGCATCCCAA CTATGGCCGACTTCGTGCAGATTGAGAATTGTAATGTCACGTTCGTGAATATATCTCGGTCCGAACTGCA GACTATCGTTCCAGAATATATTGACGTAAATAAAACGCTCCAGGAGCTGAGTTACAAGTTGCCAAACTAC ACAGTCCCTGATCTGGTCGTGGAACAATACAATCAGACTATCCTGAACCTGACTAGCGAGATTAGCACCC TGGAGAATAAGTCCGCTGAACTGAACTATACGGTTCAGAAACTGCAGACGCTGATTGACAACATAAACAG CACCCTGGTGGACCTGAAGTGGCTGAACCGCGTTGAAACTTACATCAAGTGGCCGTGGTGGGTCTGGCTT TGTATTTCTGTCGTGCTGATTTTCGTGGTCTCAATGTTGCTGCTGTGTTGTTGCTCAACTGGTTGCTGTG GATTTTTTTCATGTTTCGCTTCCAGCATCAGAGGATGTTGCGAGAGTACCAAGCTGCCCTATTACGATGT GGAAAAAATCCACATACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100563 representing NP_073551 Red=Cloning sites Green=Tags MFVLLVAYALLHIAGCQTTNGLNTSYSVCNGCVGYSENVFAVESGGYIPSDFAFNNWFLLTNTSSVVDGV VRSFQPLLLNCLWSVSGLRFTTGFVYFNGTGRGDCKGFSSDVLSDVIRYNLNFEENLRRGTILFKTSYGV VVFYCTNNTLVSGDAHIPFGTVLGNFYCFVNTTIGNETTSAFVGALPKTVREFVISRTGHFYINGYRYFT LGNVEAVNFNVTTAETTDFCTVALASYADVLVNVSQTSIANIIYCNSVINRLRCDQLSFDVPDGFYSTSP IQSVELPVSIVSLPVYHKHTFIVLYVDFKPQSGGGKCFNCYPAGVNITLANFNETKGPLCVDTSHFTTKY VAVYANVGRWSASINTGNCPFSFGKVNNFVKFGSVCFSLKDIPGGCAMPIVANWAYSKYYTIGSLYVSWS DGDGITGVPQPVEGVSSFMNVTLDKCTKYNIYDVSGVGVIRVSNDTFLNGITYTSTSGNLLGFKDVTKGT IYSITPCNPPDQLVVYQQAVVGAMLSENFTSYGFSNVVELPKFFYASNGTYNCTDAVLTYSSFGVCADGS IIAVQPRNVSYDSVSAIVTANLSIPSNWTTSVQVEYLQITSTPIVVDCSTYVCNGNVRCVELLKQYTSAC KTIEDALRNSARLESADVSEMLTFDKKAFTLANVSSFGDYNLSSVIPSLPTSGSRVAGRSAIEDILFSKL VTSGLGTVDADYKKCTKGLSIADLACAQYYNGIMVLPGVADAERMAMYTGSLIGGIALGGLTSAVSIPFS LAIQARLNYVALQTDVLQENQKILAASFNKAMTNIVDAFTGVNDAITQTSQALQTVATALNKIQDVVNQQ GNSLNHLTSQLRQNFQAISSSIQAIYDRLDTIQADQQVDRLITGRLAALNVFVSHTLTKYTEVRASRQLA QQKVNECVKSQSKRYGFCGNGTHIFSIVNAAPEGLVFLHTVLLPTQYKDVEAWSGLCVDGTNGYVLRQPN LALYKEGNYYRITSRIMFEPRIPTMADFVQIENCNVTFVNISRSELQTIVPEYIDVNKTLQELSYKLPNY TVPDLVVEQYNQTILNLTSEISTLENKSAELNYTVQKLQTLIDNINSTLVDLKWLNRVETYIKWPWWVWL CISVVLIFVVSMLLLCCCSTGCCGFFSCFASSIRGCCESTKLPYYDVEKIHIQ TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_002645
ORF Size	3519 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_002645.1, NP_073551
RefSeq ORF	3519 bp
Locus ID	918758
MW	128.6 kDa

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