NS1 (NC_007455) Virus Tagged ORF Clone

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100557 represents NCBI reference of YP_338086 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCATTCAATCCGCCTGTGATAAGAGCCTTCAGCCAGCCTGCCTTTACTTATGTGTTTAAGTTCCCCT ATCCACAGTGGAAAGAGAAAGAGTGGCTGCTTCACGCCCTCCTGGCCCACGGAACCGAGCAGTCCATGAT TCAGCTGCGAAACTGCGCCCCTCATCCAGACGAAGACATTATTCGAGATGACCTTTTGATCTCACTGGAA GACCGGCATTTTGGTGCAGTACTGTGTAAAGCCGTTTATATGGCCACAACTACACTTATGTCCCACAAGC AGCGAAACATGTTCCCCAGATGTGATATCATTGTGCAGTCCGAACTCGGCGAGAAAAATCTGCACTGTCA CATTATAGTGGGAGGCGAGGGCCTGAGCAAGCGCAACGCAAAGTCAAGTTGTGCACAATTCTATGGACTG ATTTTGGCTGAGATAATTCAGCGCTGTAAATCCCTTTTGGCAACCCGCCCCTTCGAACCCGAGGAAGCTG ACATTTTTCATACTCTGAAAAAGGCTGAAAGAGAGGCATGGGGCGGAGTGACAGGCGGAAACATGCAGAT CTTGCAGTATAGGGACAGACGAGGCGACCTCCACGCTCAAACAGTAGATCCCCTGCGGTTCTTCAAGAAT TATCTGCTTCCCAAAAATAGGTGTATCTCCTCATACTCCAAGCCCGATGTGTGCACGTCACCTGACAACT GGTTCATTCTGGCCGAAAAGACTTACAGCCATACTTTGATAAACGGACTGCCTCTGCCGGAGCACTATCG GAAGAATTATCACGCCACTCTGGACAACGAGGTAATACCGGGACCTCAGACGATGGCCTACGGAGGCAGG GGACCCTGGGAGCACCTGCCCGAAGTGGGCGATCAGCGGCTCGCAGCAAGCAGCGTGTCCACAACTTATA AACCAAATAAGAAAGAAAAGCTTATGCTGAACCTTCTGGACAAGTGCAAGGAACTGAACTTGTTGGTTTA TGAAGACCTCGTGGCAAATTGCCCTGAGCTTCTCCTGATGCTGGAAGGCCAGCCTGGCGGGGCTAGACTT ATTGAACAGGTGTTGGGCATGCACCACATTAATGTGTGCTCTAACTTCACCGCGCTTACCTACCTCTTCC ACCTGCACCCTGTGACTTCCCTGGACTCCGACAATAAGGCACTGCAGCTGCTGCTGATCCAGGGGTACAA TCCTCTTGCAGTAGGCCACGCTCTGTGTTGCGTACTCAACAAACAGTTTGGGAAGCAAAACACTGTGTGC TTTTATGGTCCGGCATCCACTGGGAAGACCAACATGGCAAAAGCTATTGTCCAGGGGATCCGGCTCTATG GTTGTGTGAACCATCTGAACAAAGGGTTTGTGTTTAATGACTGTCGCCAGAGGCTGGTCGTGTGGTGGGA AGAGTGTCTGATGCATCAAGACTGGGTGGAACCAGCCAAGTGCATCCTTGGCGGAACCGAATGCAGGATT GACGTGAAGCACAGGGACAGCGTCCTGCTGACACAAACCCCGGTAATCATCAGCACGAATCACGACATTT ATGCCGTGGTCGGAGGCAACTCCGTGTCACACGTACATGCGGCGCCCCTTAAAGAACGGGTGATTCAACT TAACTTTATGAAGCAGCTCCCGCAGACATTCGGGGAGATCACTGCCACTGAGATTGCCGCGCTTCTTCAG TGGTGCTTTAACGAGTACGATTGTACTCTCACAGGCTTCAAGCAAAAGTGGAATCTTGACAAGATTCCAA ATTCATTTCCACTGGGAGTGCTCTGTCCGACCCATTCCCAGGATTTTACCTTGCATGAGAACGGCTATTG TACTGACTGCGGGGGCTACCTCCCCCACAGCGCTGATAATAGCATGTACACAGATCGCGCGTCCGAAACA AGCACAGGAGACATCACTCCCTCAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100557 representing YP_338086 Red=Cloning sites Green=Tags MAFNPPVIRAFSQPAFTYVFKFPYPQWKEKEWLLHALLAHGTEQSMIQLRNCAPHPDEDIIRDDLLISLE DRHFGAVLCKAVYMATTTLMSHKQRNMFPRCDIIVQSELGEKNLHCHIIVGGEGLSKRNAKSSCAQFYGL ILAEIIQRCKSLLATRPFEPEEADIFHTLKKAEREAWGGVTGGNMQILQYRDRRGDLHAQTVDPLRFFKN YLLPKNRCISSYSKPDVCTSPDNWFILAEKTYSHTLINGLPLPEHYRKNYHATLDNEVIPGPQTMAYGGR GPWEHLPEVGDQRLAASSVSTTYKPNKKEKLMLNLLDKCKELNLLVYEDLVANCPELLLMLEGQPGGARL IEQVLGMHHINVCSNFTALTYLFHLHPVTSLDSDNKALQLLLIQGYNPLAVGHALCCVLNKQFGKQNTVC FYGPASTGKTNMAKAIVQGIRLYGCVNHLNKGFVFNDCRQRLVVWWEECLMHQDWVEPAKCILGGTECRI DVKHRDSVLLTQTPVIISTNHDIYAVVGGNSVSHVHAAPLKERVIQLNFMKQLPQTFGEITATEIAALLQ WCFNEYDCTLTGFKQKWNLDKIPNSFPLGVLCPTHSQDFTLHENGYCTDCGGYLPHSADNSMYTDRASET STGDITPSK TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene
ACCN	NC_007455
ORF Size	1917 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NC_007455.1, YP_338086
RefSeq ORF	1917 bp
Locus ID	3711585
MW	72.1 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.