E1A (NC_001454) Virus Tagged ORF Clone

CAT#: VC100500

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for control protein E1A [Human adenovirus F], codon optimized for human cell expression, NP_040845



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100500 represents NCBI reference of NP_040845 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGATGCTTCCTGATTTTTTTACAGGCAATTGGGATGACATGTTCCAGGGACTGCTCGAGACAGAGT
ACGTGTTCGATTTTCCGGAACCCTCTGAGGCATCTGAAGAAATGTCTCTGCACGACTTGTTCGATGTGGA
AGTCGATGGCTTCGAGGAAGATGCCAATCAGGAAGCAGTCGATGGTATGTTCCCGGAGAGGCTCCTGTCT
GAAGCAGAATCTGCAGCAGAGTCTGGCAGTGGAGACAGCGGTGTCGGCGAGGAACTTCTGCCTGTCGATC
TGGACCTGAAGTGCTATGAGGATGGCTTGCCCCCAAGCGACCCCGAAACTGACGAGGCAACAGAGGCAGA
GGAAGAAGCCGCAATGCCCACATACGTGAATGAAAATGAGAACGAACTGGTGCTTGACTGCCCTGAGAAC
CCCGGTCGCGGATGCAGGGCATGTGACTTCCACCGGGGGACTAGTGGGAACCCCGAGGCAATGTGTGCTT
TGTGCTATATGCGGCTGACCGGGCACTGCATATATTCCCCAATTTCCGATGCCGAGGGTGAGAGCGAATC
AGGCAGCCCAGAGGACACGGATTTTCCGCACCCCCTTACCGCAACCCCTCCTCACGGCATCGTCCGCACA
ATTCCTTGCCGCGTCTCTTGTCGCAGGCGGCCCGCCGTGGAGTGCATTGAAGATCTCTTGGAAGAGGACC
CTACCGACGAACCACTCAATCTTAGCCTGAAGAGGCCTAAGTGTTCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100500 representing NP_040845
Red=Cloning sites Green=Tags

MRMLPDFFTGNWDDMFQGLLETEYVFDFPEPSEASEEMSLHDLFDVEVDGFEEDANQEAVDGMFPERLLS
EAESAAESGSGDSGVGEELLPVDLDLKCYEDGLPPSDPETDEATEAEEEAAMPTYVNENENELVLDCPEN
PGRGCRACDFHRGTSGNPEAMCALCYMRLTGHCIYSPISDAEGESESGSPEDTDFPHPLTATPPHGIVRT
IPCRVSCRRRPAVECIEDLLEEDPTDEPLNLSLKRPKCS

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001454
ORF Size 747 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001454.1, NP_040845
RefSeq ORF 747 bp
Locus ID 2715915
MW 27.5 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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