E4 34K (AC_000006) Virus Tagged ORF Clone

CAT＃: VC100421

TrueORF^®

Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus D], codon optimized for human cell expression, AP_000158

Datasheet

SDS

View other clones from "Virus" (17)

Need custom modification / cloning service?
Get a free quote

CNY 3,800.00

货期*

4周

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

TA592569

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 600.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

TA50011-100

Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody

CNY 1,050.00

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

Specifications

Product Data
Type	Virus Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >The Viral ORF clone VC100421 represents NCBI reference of AP_000158 with codon optimized for human cell expression Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGACCGAAATACAGTCTAGCTCACTTCGCCATCATCCCTACCGCAGGGCTAGATTGCCCCGGAGCG ATGACGAGACACGGGCCAGCCTGACTGACCAACATCCTCTTCTGCCAGACTGCGATCACGCCGATTACCA CAACGTGAGTTCAGTGAGAGGCCTGCCCTGTGCGGCTGGATTCACCCTCCTGCAAGAGTTTCCTGTGCCG TGGGACATGATCCTGACGACTGAAGAAATTAAGATACTGAAAAGGTGCATGAGCGTATGTCTGTGTCCAG CCACATTGGATCTGGTCCGGGCGCAGATGGTCTCAGGTTATGAGCGGTGGATCCTGCACTGTCACTGCAG CAGCCCCGGGAGTCTGCAGTGCCGGGCCGGTGGCACCCTGTTGGCAGTGTGGTTCCGCAGAGTAATATAC GGCTGCATGTTCAACCAGAGATTCCCGTGGTACCGGCAGATAGTAAACCGCAACATGCCCAAGGAAATAA TGTACATGGGATCAGTGTTTATGAGAGGTAGGCACCTTATATATTGCAGAATCTGGTATGACGGCCATGT CGGCTCCATTATCCCTAATATGTCTTTCGGATGGAGTACCCTGAATTATGGGCTGTTGAACAACATGGTG ATAATGTGCTGTACCTACTGCGAGAATATGGCTGAAATCCGCATGCGCTGCTGTGCACGAAGAACCCGCC GGCTCATGTTGAAAGCAGTGGGTATTATTGTGCGGGAGACCTGCGACCCTGATCCAATCTGCAGCAGCCG AACCGAACCAAGGAGACAACGACTCCTCAGAGCACTGATGGAGCGGCACAGACCCATTCTGTTCTCTGAG TATGAGAGCGTACGCTCCTCCCGCTCTACTCGACTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >VC100421 representing AP_000158 Red=Cloning sites Green=Tags MQTEIQSSSLRHHPYRRARLPRSDDETRASLTDQHPLLPDCDHADYHNVSSVRGLPCAAGFTLLQEFPVP WDMILTTEEIKILKRCMSVCLCPATLDLVRAQMVSGYERWILHCHCSSPGSLQCRAGGTLLAVWFRRVIY GCMFNQRFPWYRQIVNRNMPKEIMYMGSVFMRGRHLIYCRIWYDGHVGSIIPNMSFGWSTLNYGLLNNMV IMCCTYCENMAEIRMRCCARRTRRLMLKAVGIIVRETCDPDPICSSRTEPRRQRLLRALMERHRPILFSE YESVRSSRSTRL TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene
ACCN	AC_000006
ORF Size	876 bp
OTI Disclaimer	The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AC_000006.1, AP_000158
RefSeq ORF	876 bp
MW	34.1 kDa

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
VC100405	Myc-DDK-tagged ORF clone of viral ORF for E1B 19K [Human adenovirus D], codon optimized for human cell expression, AP_000142	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100407	Myc-DDK-tagged ORF clone of viral ORF for pTP [Human adenovirus D], codon optimized for human cell expression, AP_000144	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,600.00
VC100408	Myc-DDK-tagged ORF clone of viral ORF for 52K [Human adenovirus D], codon optimized for human cell expression, AP_000145	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,470.00
VC100409	Myc-DDK-tagged ORF clone of viral ORF for pIIIa [Human adenovirus D], codon optimized for human cell expression, AP_000146	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,840.00
VC100410	Myc-DDK-tagged ORF clone of viral ORF for III [Human adenovirus D], codon optimized for human cell expression, AP_000147	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,270.00
VC100411	Myc-DDK-tagged ORF clone of viral ORF for pX [Human adenovirus D], codon optimized for human cell expression, AP_000148	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100412	Myc-DDK-tagged ORF clone of viral ORF for pVI [Human adenovirus D], codon optimized for human cell expression, AP_000149	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100413	Myc-DDK-tagged ORF clone of viral ORF for E3 125K [Human adenovirus D], codon optimized for human cell expression, AP_000150	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100414	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-alpha1 [Human adenovirus D], codon optimized for human cell expression, AP_000151	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100415	Myc-DDK-tagged ORF clone of viral ORF for E3 gp19K [Human adenovirus D], codon optimized for human cell expression, AP_000152	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100416	Myc-DDK-tagged ORF clone of viral ORF for E3 CR1-gamma1 [Human adenovirus D], codon optimized for human cell expression, AP_000153	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100417	Myc-DDK-tagged ORF clone of viral ORF for E3 RID-beta [Human adenovirus D], codon optimized for human cell expression, AP_000154	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100418	Myc-DDK-tagged ORF clone of viral ORF for E3 147K [Human adenovirus D], codon optimized for human cell expression, AP_000155	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100419	Myc-DDK-tagged ORF clone of viral ORF for U exon, partial [Human adenovirus D], codon optimized for human cell expression, AP_000156	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100420	Myc-DDK-tagged ORF clone of viral ORF for fiber [Human adenovirus D], codon optimized for human cell expression, AP_000157	10 ug 产品选项验证失败所有选项都是必需的	CNY 4,370.00
VC100422	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF4 [Human adenovirus D], codon optimized for human cell expression, AP_000159	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00
VC100423	Myc-DDK-tagged ORF clone of viral ORF for E4 ORF3 [Human adenovirus D], codon optimized for human cell expression, AP_000160	10 ug 产品选项验证失败所有选项都是必需的	CNY 3,800.00

其它E4 34K产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies