L3 (NC_001405) Virus Tagged ORF Clone

CAT#: VC100383

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus C], codon optimized for human cell expression, NP_040525



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CNY 14,540.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100383 represents NCBI reference of NP_040525 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAACTCCCTCCATGATGCCTCAGTGGTCATACATGCACATCTCTGGTCAAGATGCCAGTGAGTACC
TCTCACCAGGCCTCGTACAGTTTGCAAGGGCCACTGAGACATATTTTAGTCTCAACAACAAATTCCGAAA
CCCAACCGTGGCCCCTACACATGATGTTACAACAGATCGGAGCCAGCGACTGACGCTCCGGTTCATCCCT
GTAGATCGGGAGGACACCGCCTACTCTTACAAGGCCAGATTTACTCTCGCTGTGGGAGATAACCGAGTCT
TGGACATGGCAAGCACTTATTTCGACATTAGAGGCGTTCTGGATAGAGGGCCAACTTTCAAGCCATACTC
CGGCACCGCTTATAATGCACTGGCACCAAAAGGTGCTCCCAATAGCTGCGAGTGGGAGCAGACTGAGGAT
TCTGGACGGGCCGTTGCTGAGGACGAAGAAGAAGAGGATGAAGACGAGGAGGAAGAGGAGGAAGAGCAGA
ACGCTCGGGACCAGGCCACTAAGAAAACTCATGTATATGCCCAGGCCCCTCTGTCTGGCGAGACTATAAC
TAAGAGCGGTCTCCAGATCGGTTCCGACAACGCAGAGACGCAGGCCAAACCCGTCTATGCCGATCCAAGT
TACCAGCCAGAACCCCAGATTGGGGAGAGCCAGTGGAATGAAGCCGACGCCAACGCTGCCGGTGGCCGGG
TGCTCAAAAAGACGACGCCCATGAAACCCTGTTACGGAAGCTACGCTCGGCCCACTAATCCTTTTGGCGG
GCAGAGCGTCCTCGTACCGGATGAAAAAGGCGTCCCGCTTCCTAAGGTGGACCTGCAATTTTTCTCCAAC
ACCACTAGCCTGAATGACAGGCAAGGTAACGCCACGAAGCCCAAGGTGGTGCTTTACTCCGAGGATGTCA
ATATGGAGACGCCCGATACCCATCTTTCCTACAAACCAGGCAAGGGAGATGAAAATTCAAAGGCCATGCT
GGGCCAGCAAAGCATGCCCAACAGACCCAATTACATTGCTTTCCGAGACAACTTCATCGGACTGATGTAC
TATAATAGCACCGGCAACATGGGAGTGTTGGCTGGACAGGCTTCCCAGTTGAATGCAGTTGTCGATCTCC
AGGACCGCAACACTGAACTGAGCTATCAGCTCCTCCTCGATAGTATCGGAGATAGAACCAGGTATTTCTC
TATGTGGAATCAGGCCGTGGATAGCTACGACCCCGACGTCCGAATTATCGAAAACCACGGCACCGAGGAT
GAGCTCCCTAACTACTGCTTCCCCCTGGGCGGGATCGGCGTTACCGACACGTACCAAGCAATTAAGGCGA
ATGGTAATGGAAGCGGGGATAACGGTGACACAACCTGGACAAAAGATGAAACTTTCGCAACCAGAAATGA
AATTGGGGTGGGGAACAATTTCGCGATGGAAATCAATTTGAACGCTAATCTGTGGAGGAATTTTCTGTAC
AGCAATATTGCGCTGTATCTGCCCGACAAACTGAAGTACAACCCAACTAATGTAGAGATCAGCGACAATC
CAAACACTTATGACTACATGAACAAGCGCGTGGTCGCCCCCGGCCTTGTAGACTGTTATATTAACCTCGG
CGCCCGCTGGAGTTTGGACTACATGGACAACGTGAATCCCTTCAACCACCACCGCAACGCAGGGCTGAGG
TACAGAAGTATGCTTCTGGGGAATGGCCGGTACGTACCCTTTCATATTCAGGTACCTCAGAAATTCTTTG
CAATCAAGAATCTTCTCCTCCTGCCAGGTTCCTACACATACGAATGGAATTTCAGGAAGGACGTCAATAT
GGTGCTGCAGAGCTCACTGGGTAACGACCTGAGAGTTGATGGCGCAAGTATCAAGTTCGATTCCATTTGC
CTGTACGCTACTTTTTTCCCCATGGCACATAACACCGCCAGCACCTTGGAGGCTATGCTTCGCAACGATA
CTAATGATCAGTCATTCAATGATTACTTGTCTGCTGCTAACATGCTGTATCCAATCCCGGCTAATGCAAC
CAATGTCCCGATTAGCATACCGTCCAGGAATTGGGCCGCCTTTCGCGGGTGGGCGTTTACACGGTTGAAG
ACAAAAGAAACACCTAGTCTGGGTAGTGGCTATGATCCTTATTATACGTATTCCGGCAGCATTCCATATC
TGGATGGAACATTTTACCTCAACCATACTTTCAAGAAGGTGGCTATTACTTTCGACTCTAGTGTGTCTTG
GCCGGGAAACGATAGACTTCTGACGCCCAATGAGTTCGAAATTAAGCGCTCTGTGGATGGCGAGGGATAC
AATGTGGCCCAGTGCAACATGACGAAGGACTGGTTTCTTGTCCAGATGCTGGCAAACTATAACATTGGTT
ACCAGGGCTTTTATATTCCAGAGTCCTACAAAGACAGAATGTACTCATTTTTTAGGAACTTCCAACCTAT
GAGCCGCCAGGTTGTGGACGACACTAAGTACAAGGAGTACCAGCAGGTCGGCATACTGCACCAGCACAAT
AATTCTGGCTTCGTCGGGTACCTGGCCCCAACCATGCGGGAGGGCCAGGCGTACCCTGCCAATGTGCCCT
ATCCTCTGATTGGCAAAACCGCGGTGGACAGCATTACCCAAAAAAAGTTCCTGTGCGACAGGACCCTGTG
GAGAATTCCCTTCTCAAGTAATTTCATGAGCATGGGAGCCCTGACCGACCTTGGCCAAAACCTGCTCTAC
GCAAACTCAGCACACGCTCTGGACATGACCTTCGAAGTTGACCCAATGGATGAACCCACGCTGCTGTACG
TACTGTTTGAGGTTTTTGATGTGGTGCGGGTCCATCAGCCTCACCGCGGCGTGATAGAGACGGTTTATCT
GCGAACACCTTTTTCCGCCGGCAATGCAACCACT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100383 representing NP_040525
Red=Cloning sites Green=Tags

MATPSMMPQWSYMHISGQDASEYLSPGLVQFARATETYFSLNNKFRNPTVAPTHDVTTDRSQRLTLRFIP
VDREDTAYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPTFKPYSGTAYNALAPKGAPNSCEWEQTED
SGRAVAEDEEEEDEDEEEEEEEQNARDQATKKTHVYAQAPLSGETITKSGLQIGSDNAETQAKPVYADPS
YQPEPQIGESQWNEADANAAGGRVLKKTTPMKPCYGSYARPTNPFGGQSVLVPDEKGVPLPKVDLQFFSN
TTSLNDRQGNATKPKVVLYSEDVNMETPDTHLSYKPGKGDENSKAMLGQQSMPNRPNYIAFRDNFIGLMY
YNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLLLDSIGDRTRYFSMWNQAVDSYDPDVRIIENHGTED
ELPNYCFPLGGIGVTDTYQAIKANGNGSGDNGDTTWTKDETFATRNEIGVGNNFAMEINLNANLWRNFLY
SNIALYLPDKLKYNPTNVEISDNPNTYDYMNKRVVAPGLVDCYINLGARWSLDYMDNVNPFNHHRNAGLR
YRSMLLGNGRYVPFHIQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASIKFDSIC
LYATFFPMAHNTASTLEAMLRNDTNDQSFNDYLSAANMLYPIPANATNVPISIPSRNWAAFRGWAFTRLK
TKETPSLGSGYDPYYTYSGSIPYLDGTFYLNHTFKKVAITFDSSVSWPGNDRLLTPNEFEIKRSVDGEGY
NVAQCNMTKDWFLVQMLANYNIGYQGFYIPESYKDRMYSFFRNFQPMSRQVVDDTKYKEYQQVGILHQHN
NSGFVGYLAPTMREGQAYPANVPYPLIGKTAVDSITQKKFLCDRTLWRIPFSSNFMSMGALTDLGQNLLY
ANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRVHQPHRGVIETVYLRTPFSAGNATT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_001405
ORF Size 2904 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_001405.1, NP_040525
RefSeq ORF 2904 bp
Locus ID 2652998
MW 109.2 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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