L3 (NC_001405) Virus Tagged ORF Clone
CAT#: VC100383
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus C], codon optimized for human cell expression, NP_040525
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CNY 14,540.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100383 represents NCBI reference of NP_040525 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAACTCCCTCCATGATGCCTCAGTGGTCATACATGCACATCTCTGGTCAAGATGCCAGTGAGTACC TCTCACCAGGCCTCGTACAGTTTGCAAGGGCCACTGAGACATATTTTAGTCTCAACAACAAATTCCGAAA CCCAACCGTGGCCCCTACACATGATGTTACAACAGATCGGAGCCAGCGACTGACGCTCCGGTTCATCCCT GTAGATCGGGAGGACACCGCCTACTCTTACAAGGCCAGATTTACTCTCGCTGTGGGAGATAACCGAGTCT TGGACATGGCAAGCACTTATTTCGACATTAGAGGCGTTCTGGATAGAGGGCCAACTTTCAAGCCATACTC CGGCACCGCTTATAATGCACTGGCACCAAAAGGTGCTCCCAATAGCTGCGAGTGGGAGCAGACTGAGGAT TCTGGACGGGCCGTTGCTGAGGACGAAGAAGAAGAGGATGAAGACGAGGAGGAAGAGGAGGAAGAGCAGA ACGCTCGGGACCAGGCCACTAAGAAAACTCATGTATATGCCCAGGCCCCTCTGTCTGGCGAGACTATAAC TAAGAGCGGTCTCCAGATCGGTTCCGACAACGCAGAGACGCAGGCCAAACCCGTCTATGCCGATCCAAGT TACCAGCCAGAACCCCAGATTGGGGAGAGCCAGTGGAATGAAGCCGACGCCAACGCTGCCGGTGGCCGGG TGCTCAAAAAGACGACGCCCATGAAACCCTGTTACGGAAGCTACGCTCGGCCCACTAATCCTTTTGGCGG GCAGAGCGTCCTCGTACCGGATGAAAAAGGCGTCCCGCTTCCTAAGGTGGACCTGCAATTTTTCTCCAAC ACCACTAGCCTGAATGACAGGCAAGGTAACGCCACGAAGCCCAAGGTGGTGCTTTACTCCGAGGATGTCA ATATGGAGACGCCCGATACCCATCTTTCCTACAAACCAGGCAAGGGAGATGAAAATTCAAAGGCCATGCT GGGCCAGCAAAGCATGCCCAACAGACCCAATTACATTGCTTTCCGAGACAACTTCATCGGACTGATGTAC TATAATAGCACCGGCAACATGGGAGTGTTGGCTGGACAGGCTTCCCAGTTGAATGCAGTTGTCGATCTCC AGGACCGCAACACTGAACTGAGCTATCAGCTCCTCCTCGATAGTATCGGAGATAGAACCAGGTATTTCTC TATGTGGAATCAGGCCGTGGATAGCTACGACCCCGACGTCCGAATTATCGAAAACCACGGCACCGAGGAT GAGCTCCCTAACTACTGCTTCCCCCTGGGCGGGATCGGCGTTACCGACACGTACCAAGCAATTAAGGCGA ATGGTAATGGAAGCGGGGATAACGGTGACACAACCTGGACAAAAGATGAAACTTTCGCAACCAGAAATGA AATTGGGGTGGGGAACAATTTCGCGATGGAAATCAATTTGAACGCTAATCTGTGGAGGAATTTTCTGTAC AGCAATATTGCGCTGTATCTGCCCGACAAACTGAAGTACAACCCAACTAATGTAGAGATCAGCGACAATC CAAACACTTATGACTACATGAACAAGCGCGTGGTCGCCCCCGGCCTTGTAGACTGTTATATTAACCTCGG CGCCCGCTGGAGTTTGGACTACATGGACAACGTGAATCCCTTCAACCACCACCGCAACGCAGGGCTGAGG TACAGAAGTATGCTTCTGGGGAATGGCCGGTACGTACCCTTTCATATTCAGGTACCTCAGAAATTCTTTG CAATCAAGAATCTTCTCCTCCTGCCAGGTTCCTACACATACGAATGGAATTTCAGGAAGGACGTCAATAT GGTGCTGCAGAGCTCACTGGGTAACGACCTGAGAGTTGATGGCGCAAGTATCAAGTTCGATTCCATTTGC CTGTACGCTACTTTTTTCCCCATGGCACATAACACCGCCAGCACCTTGGAGGCTATGCTTCGCAACGATA CTAATGATCAGTCATTCAATGATTACTTGTCTGCTGCTAACATGCTGTATCCAATCCCGGCTAATGCAAC CAATGTCCCGATTAGCATACCGTCCAGGAATTGGGCCGCCTTTCGCGGGTGGGCGTTTACACGGTTGAAG ACAAAAGAAACACCTAGTCTGGGTAGTGGCTATGATCCTTATTATACGTATTCCGGCAGCATTCCATATC TGGATGGAACATTTTACCTCAACCATACTTTCAAGAAGGTGGCTATTACTTTCGACTCTAGTGTGTCTTG GCCGGGAAACGATAGACTTCTGACGCCCAATGAGTTCGAAATTAAGCGCTCTGTGGATGGCGAGGGATAC AATGTGGCCCAGTGCAACATGACGAAGGACTGGTTTCTTGTCCAGATGCTGGCAAACTATAACATTGGTT ACCAGGGCTTTTATATTCCAGAGTCCTACAAAGACAGAATGTACTCATTTTTTAGGAACTTCCAACCTAT GAGCCGCCAGGTTGTGGACGACACTAAGTACAAGGAGTACCAGCAGGTCGGCATACTGCACCAGCACAAT AATTCTGGCTTCGTCGGGTACCTGGCCCCAACCATGCGGGAGGGCCAGGCGTACCCTGCCAATGTGCCCT ATCCTCTGATTGGCAAAACCGCGGTGGACAGCATTACCCAAAAAAAGTTCCTGTGCGACAGGACCCTGTG GAGAATTCCCTTCTCAAGTAATTTCATGAGCATGGGAGCCCTGACCGACCTTGGCCAAAACCTGCTCTAC GCAAACTCAGCACACGCTCTGGACATGACCTTCGAAGTTGACCCAATGGATGAACCCACGCTGCTGTACG TACTGTTTGAGGTTTTTGATGTGGTGCGGGTCCATCAGCCTCACCGCGGCGTGATAGAGACGGTTTATCT GCGAACACCTTTTTCCGCCGGCAATGCAACCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100383 representing NP_040525
Red=Cloning sites Green=Tags MATPSMMPQWSYMHISGQDASEYLSPGLVQFARATETYFSLNNKFRNPTVAPTHDVTTDRSQRLTLRFIP VDREDTAYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPTFKPYSGTAYNALAPKGAPNSCEWEQTED SGRAVAEDEEEEDEDEEEEEEEQNARDQATKKTHVYAQAPLSGETITKSGLQIGSDNAETQAKPVYADPS YQPEPQIGESQWNEADANAAGGRVLKKTTPMKPCYGSYARPTNPFGGQSVLVPDEKGVPLPKVDLQFFSN TTSLNDRQGNATKPKVVLYSEDVNMETPDTHLSYKPGKGDENSKAMLGQQSMPNRPNYIAFRDNFIGLMY YNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLLLDSIGDRTRYFSMWNQAVDSYDPDVRIIENHGTED ELPNYCFPLGGIGVTDTYQAIKANGNGSGDNGDTTWTKDETFATRNEIGVGNNFAMEINLNANLWRNFLY SNIALYLPDKLKYNPTNVEISDNPNTYDYMNKRVVAPGLVDCYINLGARWSLDYMDNVNPFNHHRNAGLR YRSMLLGNGRYVPFHIQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMVLQSSLGNDLRVDGASIKFDSIC LYATFFPMAHNTASTLEAMLRNDTNDQSFNDYLSAANMLYPIPANATNVPISIPSRNWAAFRGWAFTRLK TKETPSLGSGYDPYYTYSGSIPYLDGTFYLNHTFKKVAITFDSSVSWPGNDRLLTPNEFEIKRSVDGEGY NVAQCNMTKDWFLVQMLANYNIGYQGFYIPESYKDRMYSFFRNFQPMSRQVVDDTKYKEYQQVGILHQHN NSGFVGYLAPTMREGQAYPANVPYPLIGKTAVDSITQKKFLCDRTLWRIPFSSNFMSMGALTDLGQNLLY ANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRVHQPHRGVIETVYLRTPFSAGNATT TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001405 |
ORF Size | 2904 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001405.1, NP_040525 |
RefSeq ORF | 2904 bp |
Locus ID | 2652998 |
MW | 109.2 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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