L4 (NC_011202) Virus Tagged ORF Clone

CAT#: VC100301

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus B], codon optimized for human cell expression, YP_002213815



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CNY 12,350.00


货期*
6周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100301 represents NCBI reference of YP_002213815 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACTCAGCCTCTCCTGTCTACATCACCAAGGGTGCCCAGTCACCTTGTCTTGTCAGATGAAGAGA
AGGAGCAGAGTTTGTCAATTCCCCCTCCTAGCCCGGCGACCACCAGCACCCTTGAGGACAAGGAGGTGGA
TGCTAGTCATGACATGCAGAACAAGAAAGCCAAGGAATCAGAAACTGATATTGAACAGGACCCTGGCTAC
GTGACACCCGTAGAACACGAAGAGGAGCTGAAAAGGTTTTTGGAGAGAGAGGACGAGAATTGCCCTAAGC
AGAGGGCTGACAACTACCACCAGGATGCAGGGAATCGGGACCAGAATACCGACTATCTGATCGGACTGGA
CGGGGAAGATGCTCTGCTGAAGCATCTGGCCAGACAATCCCTGATCGTTAAAGACGCCCTGCTTGATAGA
ACCGAAGTGCCAATCAGTGTGGAAGAGTTGTCCCGCGCCTATGAGCTGAACCTGTTTTCTCCCAGGACTC
CCCCCAAAAGACAGCCAAACGGGACGTGTGAACCGAATCCCAGACTTAATTTCTATCCCGCTTTCGCCGT
TCCAGAGGTATTGGCTACTTATCACATCTTCTTTAAGAATCAGAAGATCCCAGTAAGTTGTCGGGCCAAC
CGAACAAGGGCAGACGCCTTGTTGAATCTTGGCCCCGGTAGCAGACTGCCAGACATTGCTTCTCTGGAGG
AAGTGCCCAAGATCTTTGAAGGCCTGGGCAACAATGAAACACGCGCTGCAAATGCCCTTCAGAAAGGCGA
GAATGGCATGGATGAGCACCACAGCGTGCTGGTGGAGCTGGAAGGTGACAACGCTCGGCTGGCGGTCCTG
AAAAGGTCTATCGAAGTCACGCATTTCGCCTATCCTGCTGTCAATCTGCCGCCAAAAGTAATGACTGCCG
TCATGGATCAGTTGCTCATCAAGCGAGCCAGCCCCCTGAGTGAGGACATGCACGACCCAGATGCCTGTGA
TGAGGGGAAACCTGTGGTAAGTGATGAGCAACTGACTCGCTGGCTGGGAACGGACAGCCCGCGGGACTTG
GAGGAAAGGCGGAAATTGATGATGGCCGTCGTGCTGGTCACCGTGGAGCTCGAGTGCCTGAGGAGGTTTT
TCACTGACAGTGAAACGCTGAGGAAACTGGAAGAGAACCTGCACTATACTTTCCGACACGGTTTCGTGAG
ACAGGCTTGCAAAATATCTAACGTAGAGCTTACTAACTTGGTGTCATACATGGGTATCCTCCACGAAAAC
AGATTGGGACAGAGTGTTCTGCACTCCACACTTAAAGGCGAAGCAAGGAGGGACTATATCCGCGATTGCG
TCTATTTGTACCTTTGTCATACCTGGCAAACAGGCATGGGCGTATGGCAACAGTGCCTGGAAGAACAGAA
CTTGAAGGAGCTGGATAAGTTGCTTCAGAAAAGCTTGAAGGTCTTGTGGACCGGATTCGATGAACGAACT
GTTGCTAGCGATCTGGCCGACCTGATATTCCCCGAGAGATTGCGGGTTACCCTGCGCAATGGCCTGCCCG
ATTTTATGTCCCAGTCAATGCTGAACAATTTTAGGAGTTTCATTCTGGAACGCTCCGGCATACTGCCCGC
TACCTGCTGTGCCCTGCCCAGCGATTTTGTGCCCCTCACATATCGAGAGTGCCCCCCTCCCTTGTGGAGC
CACTGCTATCTGTTTCGACTCGCAAACTATCTCTCCTATCATTCTGATGTGATCGAGGATGTGTCCGGCG
ACGGCCTCCTTGAGTGCCATTGCAGATGTAATCTTTGCACCCCACATAGGTCCCTCGCGTGTAACCCCCA
GCTCATGAGCGAAACTCAGATTATTGGCACTTTCGAGCTGCAGGGGCCTAGCTCCCAGGGTGACGGCTCC
AGCCCAGGACAGTCACTGAAACTGACCCCCGGCCTGTGGACAAGCGCCTACCTTAGGAAATTCGCTCCTG
AAGATTACCACCCATATGAGATTAAATTTTACGAGGACCAGAGTCAGCCTCCCAAAGCCGAACTGTCTGC
TTGCGTGATCACGCAGGGCGCGATCCTTGCGCAGCTGCAGGCCATCCAGAAATCTCGGCAGGAGTTTCTT
CTGAAAAAGGGAAAAGGGGTGTATCTCGATCCTCAGACCGGCGAAGAGCTGAATACCAGATTTCCACAAG
ACGTGCCCACCACCAGAAAACAGGAGGTTGAGGGTGCAGCAGCCGCCCCTCGGAGGTACGGAGGCAGACT
TGGGCAATCTGGTCGCGGAGGAGGCGGCGGCCAGTCTGGGGGTCAATCTGGAGGCAGACAATTCGGTGGA
GGCAAACGCGGGGGCAGAGGAGGAGGCAGATCAAATAGACGCCAGACGGTCATCCTTGGGTGTGGCGACA
AGCAGCAGCGATACCATCTCAGAAGCGAGTCAAGGAACCCCGCCGCTAGCCAGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100301 representing YP_002213815
Red=Cloning sites Green=Tags

METQPLLSTSPRVPSHLVLSDEEKEQSLSIPPPSPATTSTLEDKEVDASHDMQNKKAKESETDIEQDPGY
VTPVEHEEELKRFLEREDENCPKQRADNYHQDAGNRDQNTDYLIGLDGEDALLKHLARQSLIVKDALLDR
TEVPISVEELSRAYELNLFSPRTPPKRQPNGTCEPNPRLNFYPAFAVPEVLATYHIFFKNQKIPVSCRAN
RTRADALLNLGPGSRLPDIASLEEVPKIFEGLGNNETRAANALQKGENGMDEHHSVLVELEGDNARLAVL
KRSIEVTHFAYPAVNLPPKVMTAVMDQLLIKRASPLSEDMHDPDACDEGKPVVSDEQLTRWLGTDSPRDL
EERRKLMMAVVLVTVELECLRRFFTDSETLRKLEENLHYTFRHGFVRQACKISNVELTNLVSYMGILHEN
RLGQSVLHSTLKGEARRDYIRDCVYLYLCHTWQTGMGVWQQCLEEQNLKELDKLLQKSLKVLWTGFDERT
VASDLADLIFPERLRVTLRNGLPDFMSQSMLNNFRSFILERSGILPATCCALPSDFVPLTYRECPPPLWS
HCYLFRLANYLSYHSDVIEDVSGDGLLECHCRCNLCTPHRSLACNPQLMSETQIIGTFELQGPSSQGDGS
SPGQSLKLTPGLWTSAYLRKFAPEDYHPYEIKFYEDQSQPPKAELSACVITQGAILAQLQAIQKSRQEFL
LKKGKGVYLDPQTGEELNTRFPQDVPTTRKQEVEGAAAAPRRYGGRLGQSGRGGGGGQSGGQSGGRQFGG
GKRGGRGGGRSNRRQTVILGCGDKQQRYHLRSESRNPAASQQ

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_011202
ORF Size 2436 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_011202.1, YP_002213815
RefSeq ORF 2436 bp
Locus ID 6870504
MW 91.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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