hexon (NC_012959) Virus Tagged ORF Clone

CAT#: VC100161

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus 54], codon optimized for human cell expression, YP_003038612



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CNY 14,350.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100161 represents NCBI reference of YP_003038612 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGCCTTACCGCCAGAGAGAGAGCCAAGATGGCTACTCCATCAATGATGCCCCAGTGGGCTTACATGC
ACATCGCCGGACAGGACGCCTCAGAATACCTGTCTCCCGGGCTCGTCCAGTTTGCCCGAGCGACAGATAC
TTACTTTTCCCTCGGAAATAAGTTCCGCAATCCTACCGTTGCTCCCACTCACGATGTGACAACTGACCGC
TCACAGCGGCTGACCCTGCGATTCGTGCCCGTGGATAGAGAAGACACAACTTACAGTTACAAGGCCCGCT
TTACCCTCGCCGTGGGCGATAACCGCGTGCTTGATATGGCCAGTACCTATTTTGATATCAGGGGCGTGCT
GGACCGAGGACCCAGCTTTAAACCCTATAGTGGGACTGCCTATAATTCTTTGGCGCCAAAGGGGGCTCCA
AATTCCAGCCAATGGATCACTAAGCAGACCAATGCCGGTAACGAAACAACGAAAACCCACACGTACGGCG
TAGCCGCCATGGGCGGTGCAGATATCACCATTAAGGGGCTTCAAATCGGCGTCGATAAGACTGAGAATAA
AAATGAGCCTATTTACGCAAACGAAATATATCAGCCCGAACCACAGGTCGGTGAAGAGAACCTGCAGGAT
GTCGAGAACTACTACGGCGGACGGGCATTGAAGAAGGAAACAAAAATGAAACCATGCTACGGCAGTTTCG
CCAGACCCACAAACGAGAAGGGCGGACAAGCTGTGTTCAAGACCGGCAACGACGGACAGCCAACTACAGA
GCATGATATCACCATGGCATTTTTCGACACCCCAGGCGACACTAACGCCGAAGATACGGAACTTGAGGCC
GATATCGTTATGTATACGGAAAACGTAAATCTCGAGACACCAGATACACACGTGGTGTATAAACCTGGAC
CACTGGAGGACTCCTCCGAAATTAATCTTACGCAGCAATCTATGCCCAATCGCCCTAACTACATCGGATT
TCGCGACAACTTCGTGGGCCTCATGTACTACAACAGCACCGGCAATATGGGTGTGCTGGCCGGCCAGGCC
AGCCAGCTGAATGCCGTTGTGGATCTGCAAGATAGAAATACAGAGCTCTCCTACCAGCTTCTGCTTGACT
CACTGGGAGACAGGACAAGGTATTTTTCCATGTGGAATTCCGCCGTGGACTCATACGATCCGGATGTGAG
AATCATCGAAAACCACGGCGTTGAGGACGAGCTCCCGAATTATTGTTTCCCCCTGGATGGCGCCGGGACC
AACGCCACCTACCAGGGCGTGAAGGTTAAGAACGGGCAGGACGGCGATAATAACGCAGAATGGGAGAAGG
ACAACGCTGTGGCTGACCGGAACCAGATTTGTAAGGGAAACATCGTGGCCATGGAAATCAACCTGCAGGC
CAATCTGTGGAAGAGTTTCTTGTATTCTAACGTCGCACTTTACCTCCCTGATAGCTTTAAATATACTCCT
GCAAACGTGACTCTTCCTACCAATACTAATACATACGAGTACATGAACGGCCGAGTCGTGGCTCCCAGCC
TCGTGGACGCCTACGTGAATATCGGTGCCCGCTGGTCCTTGGACCCTATGGATAATGTAAACCCCTTCAA
CCACCATAGGAATGCCGGCCTCCGATACAGATCTATGCTCCTGGGCAACGGGAGGTACGTACCGTTCCAT
ATACAGGTCCCGCAGAAGTTTTTTGCCATTAAAAACCTGTTGCTGCTTCCGGGCTCTTATACCTACGAAT
GGAACTTCCGCAAGGATGTGAACATGATACTGCAGTCTAGTCTTGGAAATGATCTTCGGGTTGATGGTGC
ATCTGTCAGATTCGACTCAGTGAACCTGTACGCGACCTTTTTCCCTATGGCACACAACACTGCCAGTACC
CTCGAGGCCATGCTGCGGAACGACACCAATGACCAGTCCTTTAACGACTACCTGTCAGCTGCAAACATGC
TCTACCCCATTCCGGCCAAAGCCACCAATGTACCCATCAGTATCCCATCACGCAATTGGGCGGCTTTTCG
AGGGTGGAGCTTCACTAGGCTCAAGACCAAAGAGACGCCTAGCCTGGGCTCAGGGTTCGACCCTTATTTC
GTCTATAGCGGCTCCATACCATACTTGGACGGAACCTTTTATCTGAACCACACTTTTAAGAAAGTAAGCA
TCATGTTCGATAGCTCCGTGTCCTGGCCCGGCAACGATCGCTTGCTTACCCCTAACGAATTCGAGATCAA
GCGGTCTGTGGACGGAGAAGGCTATAACGTCGCTCAGTGTAATATGACCAAGGACTGGTTTCTCATTCAG
ATGCTCTCTCATTATAATATTGGTTATCAGGGGTTTCACGTGCCAGAGGGTTATAAAGACCGAATGTATA
GCTTCTTCAGAAACTTCCAGCCAATGAGCAGGCAGGTGGTGGACGAAATTAATTATAAGGACTACAAAGC
GGTAGCGTTGCCGTTCCAACATAACAATTCAGGCTTCACCGGCTACCTGGCTCCCACGATGCGACAGGGC
CAGCCATACCCGGCAAACTTCCCGTACCCACTGATAGGGGAGACCGCCGTACCCTCAGTGACTCAGAAGA
AATTTTTGTGCGACCGCGTGATGTGGCGAATCCCATTTAGTTCCAATTTCATGTCCATGGGAGCTCTCAC
CGACCTGGGGCAGAACATGCTTTACGCGAATAGTGCACACGCCTTGGATATGACTTTTGAAGTGGACCCA
ATGGATGAGCCTACTCTCCTGTACCTCCTCTTCGAGGTGTTTGACGTCGTCCGGGTCCACCAACCTCACC
GAGGGGTCATCGAGGCTGTCTACCTCCGCACTCCATTCTCTGCGGGCAATGCCACCACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100161 representing YP_003038612
Red=Cloning sites Green=Tags

MCLTARERAKMATPSMMPQWAYMHIAGQDASEYLSPGLVQFARATDTYFSLGNKFRNPTVAPTHDVTTDR
SQRLTLRFVPVDREDTTYSYKARFTLAVGDNRVLDMASTYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAP
NSSQWITKQTNAGNETTKTHTYGVAAMGGADITIKGLQIGVDKTENKNEPIYANEIYQPEPQVGEENLQD
VENYYGGRALKKETKMKPCYGSFARPTNEKGGQAVFKTGNDGQPTTEHDITMAFFDTPGDTNAEDTELEA
DIVMYTENVNLETPDTHVVYKPGPLEDSSEINLTQQSMPNRPNYIGFRDNFVGLMYYNSTGNMGVLAGQA
SQLNAVVDLQDRNTELSYQLLLDSLGDRTRYFSMWNSAVDSYDPDVRIIENHGVEDELPNYCFPLDGAGT
NATYQGVKVKNGQDGDNNAEWEKDNAVADRNQICKGNIVAMEINLQANLWKSFLYSNVALYLPDSFKYTP
ANVTLPTNTNTYEYMNGRVVAPSLVDAYVNIGARWSLDPMDNVNPFNHHRNAGLRYRSMLLGNGRYVPFH
IQVPQKFFAIKNLLLLPGSYTYEWNFRKDVNMILQSSLGNDLRVDGASVRFDSVNLYATFFPMAHNTAST
LEAMLRNDTNDQSFNDYLSAANMLYPIPAKATNVPISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYF
VYSGSIPYLDGTFYLNHTFKKVSIMFDSSVSWPGNDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLIQ
MLSHYNIGYQGFHVPEGYKDRMYSFFRNFQPMSRQVVDEINYKDYKAVALPFQHNNSGFTGYLAPTMRQG
QPYPANFPYPLIGETAVPSVTQKKFLCDRVMWRIPFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDP
MDEPTLLYLLFEVFDVVRVHQPHRGVIEAVYLRTPFSAGNATT

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_012959
ORF Size 2859 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_012959.1, YP_003038612
RefSeq ORF 2859 bp
MW 107.4 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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