E1B 55K (AC_000007) Virus Tagged ORF Clone

CAT#: VC100056

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for E1B 55K [Human adenovirus 2], codon optimized for human cell expression, AP_000163



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CNY 5,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100056 represents NCBI reference of AP_000163 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAGGCGCAACCCAAGCGAGCGGGGAGTGCCAGCCGGCTTTTCAGGCCACGCCTCAGTGGAGTCAG
GTGGCGAGACACAAGAATCCCCGGCAACCGTGGTTTTCAGGCCACCTGGTAACAATACCGACGGTGGGGC
GACTGCTGGAGGATCTCAGGCAGCTGCAGCGGCCGGCGCAGAACCCATGGAACCAGAGAGTCGACCTGGC
CCAAGTGGAATGAACGTTGTGCAGGTAGCCGAGCTCTTTCCTGAGTTGCGGCGCATCTTGACTATCAATG
AGGATGGTCAGGGACTGAAAGGCGTTAAACGGGAAAGAGGGGCTTCTGAGGCAACAGAGGAAGCTCGAAA
CCTCACTTTTTCTCTCATGACACGGCATCGCCCAGAGTGTGTGACTTTTCAACAGATCAAGGATAACTGT
GCCAACGAGTTGGACCTCCTTGCCCAGAAGTATAGCATTGAGCAGCTGACAACGTACTGGCTGCAACCAG
GAGACGATTTTGAGGAGGCTATAAGAGTGTACGCTAAAGTCGCGCTGAGACCTGATTGCAAATACAAGAT
TAGTAAGCTGGTGAACATCAGGAATTGTTGTTACATCAGTGGAAATGGGGCTGAGGTTGAAATAGACACC
GAGGACCGAGTGGCCTTCCGGTGTAGTATGATAAATATGTGGCCGGGGGTGTTGGGCATGGATGGAGTGG
TGATCATGAATGTTCGCTTTACAGGACCCAACTTCTCTGGAACAGTTTTCCTCGCCAACACTAACTTGAT
CCTCCATGGGGTGTCTTTCTATGGCTTTAATAATACCTGCGTGGAGGCCTGGACGGATGTGAGAGTCAGG
GGGTGTGCGTTTTACTGTTGTTGGAAGGGCGTCGTATGCCGGCCTAAGTCCCGGGCGTCCATCAAGAAAT
GCCTGTTCGAGCGGTGCACATTGGGAATTCTGTCAGAAGGCAACAGTAGAGTGAGACATAACGTCGCATC
TGACTGTGGTTGCTTTATGCTGGTCAAGTCCGTCGCAGTGATCAAACATAACATGGTGTGCGGCAATTGT
GAGGACAGAGCATCCCAAATGCTGACTTGCTCTGATGGAAATTGTCACCTGCTGAAAACCATCCACGTGG
CCTCCCATAGTAGGAAAGCATGGCCTGTGTTCGAACACAATATTTTGACACGCTGTTCTTTGCATTTGGG
GAACCGCAGAGGTGTATTTCTGCCTTACCAATGCAACCTGTCACACACCAAAATCTTGCTGGAACCAGAG
TCTATGAGCAAAGTGAATCTGAACGGCGTCTTTGACATGACAATGAAGATCTGGAAGGTACTCCGCTATG
ACGAGACAAGGACGCGGTGTAGACCTTGCGAGTGCGGGGGCAAGCACATAAGAAATCAGCCTGTGATGCT
CGACGTTACCGAGGAGTTGAGACCCGACCACCTGGTCCTTGCCTGTACACGCGCCGAGTTCGGCTCTTCC
GACGAGGACACAGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100056 representing AP_000163
Red=Cloning sites Green=Tags

MERRNPSERGVPAGFSGHASVESGGETQESPATVVFRPPGNNTDGGATAGGSQAAAAAGAEPMEPESRPG
PSGMNVVQVAELFPELRRILTINEDGQGLKGVKRERGASEATEEARNLTFSLMTRHRPECVTFQQIKDNC
ANELDLLAQKYSIEQLTTYWLQPGDDFEEAIRVYAKVALRPDCKYKISKLVNIRNCCYISGNGAEVEIDT
EDRVAFRCSMINMWPGVLGMDGVVIMNVRFTGPNFSGTVFLANTNLILHGVSFYGFNNTCVEAWTDVRVR
GCAFYCCWKGVVCRPKSRASIKKCLFERCTLGILSEGNSRVRHNVASDCGCFMLVKSVAVIKHNMVCGNC
EDRASQMLTCSDGNCHLLKTIHVASHSRKAWPVFEHNILTRCSLHLGNRRGVFLPYQCNLSHTKILLEPE
SMSKVNLNGVFDMTMKIWKVLRYDETRTRCRPCECGGKHIRNQPVMLDVTEELRPDHLVLACTRAEFGSS
DEDTD

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000007
ORF Size 1485 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000007.1, AP_000163
RefSeq ORF 1485 bp
UniProt ID P03254
MW 54.9 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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