E4 34K (AC_000017) Virus Tagged ORF Clone

CAT#: VC100031

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for E4 34K [Human adenovirus 1], codon optimized for human cell expression, AP_000529



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CNY 3,800.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100031 represents NCBI reference of AP_000529 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAACCTCTGGGGTCCCCTTCGGCATGACTCTGCGCCCAACGAGGTCCAGACTGAGCAGACGAACAC
CCTATTCCCGGGATAGGCTGCCACCATTTGAGACTGAAACTCGGGCAACCATACTGGAAGACCATCCGCT
CCTCCCTGAGTGTAACACCTTGACAATGCACAATGTCTCCTACGTTAGGGGCCTCCCCTGCTCTGTCGGT
TTTACCCTGATACAAGAATGGGTTGTGCCCTGGGACATGGTTCTCACCCGGGAAGAGCTTGTGATCCTGA
GGAAGTGCATGCATGTGTGCCTCTGTTGTGCAAACATCGATATTATGACATCCATGATGATCCACGGATA
CGAATCCTGGGCTCTCCACTGTCACTGCTCCTCACCCGGCAGCCTTCAATGTATTGCTGGGGGGCAGGTA
CTCGCAAGCTGGTTCCGAATGGTGGTCGATGGCGCAATGTTTAACCAGCGGTTCATTTGGTACCGAGAAG
TTGTGAACTATAATATGCCCAAGGAGGTAATGTTCATGTCTAGCGTCTTTATGCGAGGCCGCCATCTGAT
TTACCTGCGGCTGTGGTATGATGGTCATGTTGGTTCCGTAGTGCCCGCTATGAGTTTCGGGTATTCCGCT
CTGCACTGCGGCATCCTGAATAATATAGTGGTACTGTGCTGCAGTTACTGCGCTGATCTTAGCGAAATCA
GGGTAAGGTGTTGTGCAAGGCGGACGCGAAGACTGATGCTTCGAGCCGTCAGAATTATCGCCGAAGAAAC
CACCGCTATGCTTTATAGTTGCCGGACAGAAAGACGCAGGCAGCAATTTATTCGGGCACTGCTGCAGCAT
CATCGCCCCATCCTGATGCACGATTATGACTCAACCCCTATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100031 representing AP_000529
Red=Cloning sites Green=Tags

MTTSGVPFGMTLRPTRSRLSRRTPYSRDRLPPFETETRATILEDHPLLPECNTLTMHNVSYVRGLPCSVG
FTLIQEWVVPWDMVLTREELVILRKCMHVCLCCANIDIMTSMMIHGYESWALHCHCSSPGSLQCIAGGQV
LASWFRMVVDGAMFNQRFIWYREVVNYNMPKEVMFMSSVFMRGRHLIYLRLWYDGHVGSVVPAMSFGYSA
LHCGILNNIVVLCCSYCADLSEIRVRCCARRTRRLMLRAVRIIAEETTAMLYSCRTERRRQQFIRALLQH
HRPILMHDYDSTPM

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN AC_000017
ORF Size 882 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AC_000017.1, AP_000529
RefSeq ORF 882 bp
MW 34.1 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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