EDD (UBR5) (NM_001282873) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DD5; EDD; EDD1; HYD
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282873, the custom clone sequence may differ by one or more nucleotides ATGACGTCCATCCATTTCGTGGTTCACCCGCTGCCGGGCACCGAGGACCAGCTCAATGACAGGTTACGAG AAGTTTCTGAGAAGCTGAACAAATATAATTTAAACAGCCACCCCCCTTTGAATGTATTGGAACAGGCTAC TATTAAACAGTGTGTGGTGGGACCAAATCATGCTGCCTTTCTTCTTGAGGATGGTAGAGTTTGCAGGATT GGTTTTTCAGTACAGCCAGACAGATTGGAATTGGGTAAACCTGATAATAATGATGGGTCAAAGTTGAACA GCAACTCGGGGGCAGGGAGGACGTCAAGGCCTGGTAGGACAAGCGACTCTCCATGGTTTCTCTCAGGTTC TGAGACTCTAGGCAGGCTGGCAGGCAACACCTTAGGAAGCCGCTGGAGTTCTGGAGTGGGTGGAAGTGGT GGAGGATCCTCTGGTAGGTCATCAGCTGGAGCTCGAGATTCCCGCCGGCAGACTCGAGTTATTCGGACAG GACGGGATCGAGGGTCTGGGCTTTTGGGCAGTCAGCCCCAGCCAGTTATTCCAGCATCTGTCATTCCAGA GGAGCTGATTTCACAGGCCCAAGTTGTTTTACAAGGCAAATCCAGAAGTGTCATTATTCGAGAACTTCAG AGAACAAATCTTGATGTGAACCTTGCTGTAAATAATTTACTTAGCCGGGATGATGAAGATGGAGATGATG GGGATGATACAGCCAGCGAATCTTATTTGCCTGGAGAGGATCTTATGTCTCTCCTTGATGCCGACATTCA TTCTGCCCACCCAAGTGTCATTATTGATGCAGATGCCATGTTTTCTGAAGACATTAGCTATTTTGGTTAC CCTTCTTTTCGTCGTTCATCACTTTCCAGGCTAGGCTCATCTCGAGTTCTCCTTCTTCCCTTAGAGAGAG ACTCTGAGCTGTTGCGTGAACGTGAATCCGTTTTACGTTTACGTGAACGAAGGTGGCTTGATGGAGCCTC ATTTGATAATGAAAGGGGTTCTACCAGCAAGGAAGGAGAGCCAAACTTGGATAAGAAGAATACACCTGTT CAAAGTCCAGTATCTCTAGGAGAAGATTTGCAGTGGTGGCCTGATAAGGATGGAACAAAATTCATCTGTA TTGGGGCTCTGTATTCTGAACTTCTGGCTGTCAGCAGTAAAGGAGAACTTTATCAGTGGAAATGGAGTGA ATCTGAGCCTTACAGAAATGCCCAGAATCCTTCATTACATCATCCACGAGCAACATTTTTGGGGTTAACC AATGAAAAGATAGTCCTCCTGTCTGCAAATAGCATAAGAGCAACTGTAGCTACAGAAAATAACAAGGTTG CTACATGGGTGGATGAAACTTTAAGTTCTGTGGCTTCTAAATTAGAGCACACTGCTCAGACTTACTCTGA ACTTCAAGGAGAGCGGATAGTTTCTTTACATTGCTGTGCCCTTTACACCTGCGCTCAGCTGGAAAACAGT TTATATTGGTGGGGTGTAGTTCCTTTTAGTCAAAGGAAGAAAATGTTAGAGAAAGCTAGAGCAAAAAATA AAAAGCCTAAATCCAGTGCTGGTATTTCTTCAATGCCGAACATCACTGTTGGTACCCAGGTATGCTTGAG AAATAATCCTCTTTATCATGCTGGAGCAGTTGCATTTTCAATTAGTGCTGGGATTCCTAAAGTTGGTGTC TTAATGGAGTCAGTTTGGAATATGAATGACAGCTGTAGATTTCAACTTAGATCTCCTGAAAGCTTGAAAA ACATGGAAAAAGCTAGCAAAACTACTGAAGCTAAGCCTGAAAGTAAGCAGGAGCCAGTGAAAACAGAAAT GGGTCCTCCACCATCTCCAGCATCCACGTGTAGTGATGCATCCTCAATTGCCAGCAGTGCATCAATGCCA TACAAACGACGACGGTCAACCCCTGCACCAAAAGAAGAGGAAAAGGTGAATGAAGAGCAGTGGTCTCTTC GGGAAGTGGTTTTTGTGGAAGATGTCAAGAATGTTCCTGTTGGCAAGGTGCTAAAAGTAGATGGTGCCTA TGTTGCTGTAAAATTTCCAGGAACCTCCAGTAATACTAACTGTCAGAACAGCTCTGGTCCAGATGCTGAC CCTTCTTCTCTCCTGCAGGATTGTAGGTTACTTAGAATTGATGAATTGCAGGTTGTCAAAACTGGTGGAA CACCGAAGGTTCCCGACTGTTTCCAAAGGACTCCTAAAAAGCTTTGTATACCTGAAAAAACAGAAATATT AGCAGTGAATGTAGATTCCAAAGGTGTTCATGCTGTTCTGAAGACTGGAAATTGGGTACGATACTGTATC TTTGATCTTGCTACAGGAAAAGCAGAACAGGAAAATAATTTTCCTACAAGCAGCATTGCTTTCCTTGGTC AGAATGAGAGGAATGTAGCCATTTTCACTGCTGGACAGGAATCTCCCATTATTCTTCGAGATGGAAATGG TACCATCTACCCAATGGCCAAAGATTGCATGGGAGGAATAAGGGATCCCGATTGGCTGGATCTTCCACCT ATTAGTAGTCTTGGAATGGGTGTGCATTCTTTAATAAATCTTCCTGCCAATTCAACAATCAAAAAGAAAG CTGCTGTTATCATCATGGCTGTAGAGAAACAAACCTTAATGCAACACATTCTGCGCTGTGACTATGAGGC CTGTCGACAATATCTAATGAATCTTGAGCAAGCGGTTGTTTTAGAGCAGAATCTACAGATGCTGCAGACA TTCATCAGCCACAGATGTGATGGAAATCGAAATATTTTGCATGCTTGTGTATCAGTTTGCTTTCCAACCA GCAATAAAGAAACTAAAGAAGAAGAGGAAGCGGAGCGTTCTGAAAGAAATACATTTGCAGAAAGGCTTTC TGCTGTTGAGGCCATTGCAAATGCAATATCAGTTGTTTCAAGTAATGGCCCAGGTAATCGGGCTGGATCA TCAAGTAGCCGAAGTTTGAGATTACGGGAAATGATGAGACGTTCGTTGAGAGCAGCTGGTTTGGGTAGAC ATGAAGCTGGAGCTTCATCCAGTGACCACCAGGATCCAGTTTCACCCCCCATAGCTCCCCCTAGTTGGGT TCCTGACCCTCCTGCGATGGATCCTGATGGTGACATTGATTTTATCCTGGCCCCCGCTGTGGGATCTCTT ACCACAGCAGCAACCGGTACTGGTCAAGGACCAAGCACCTCCACTATTCCAGGTCCTTCCACAGAGCCAT CTGTAGTAGAATCCAAGGATCGAAAGGCGAATGCTCATTTTATATTGAAATTGTTATGTGACAGTGTGGT TCTCCAGCCCTATCTACGAGAACTTCTTTCTGCCAAGGATGCAAGAGGGATGACCCCATTTATGTCAGCT GTAAGTGGCCGAGCTTATCCTGCTGCAATTACCATCTTAGAAACTGCTCAGAAAATTGCAAAAGCTGAAA TATCCTCAAGTGAAAAAGAGGAAGATGTATTCATGGGAATGGTTTGCCCATCAGGTACCAACCCTGATGA CTCTCCTTTATATGTTTTATGTTGTAATGACACTTGCAGTTTTACATGGACTGGAGCAGAGCACATTAAC CAGGATATTTTTGAGTGTCGAACTTGTGGCTTGCTGGAGTCACTGTGTTGTTGTACGGAATGTGCAAGGG TTTGTCATAAAGGTCATGATTGCAAACTCAAACGGACATCACCAACAGCCTACTGTGATTGTTGGGAGAA ATGTAAATGTAAAACTCTTATTGCTGGACAGAAATCTGCTCGTCTTGATCTACTTTATCGCCTGCTCACT GCTACTAATCTGGTTACTCTGCCAAACAGCAGGGGAGAGCACCTCTTACTATTCTTAGTACAGACAGTCG CAAGGCAGACGGTGGAGCATTGTCAATACAGGCCACCTCGAATCAGGGAAGATCGTAACCGAAAAACAGC CAGTCCTGAAGATTCAGATATGCCAGATCATGATTTAGAGCCTCCAAGATTTGCCCAGCTTGCATTGGAG CGTGTTCTACAGGACTGGAATGCCTTGAAATCTATGATTATGTTTGGGTCGCAGGAGAATAAAGACCCTC TTAGTGCCAGCAGTAGAATAGGCCATCTTTTGCCAGAAGAGCAAGTATACCTCAATCAGCAAAGTGGCAC AATTCGGCTGGACTGTTTCACTCATTGCCTTATAGTTAAGTGTACAGCAGATATTTTGCTTTTAGATACT CTACTAGGTACACTAGTGAAAGAACTCCAAAACAAATATACACCTGGACGTAGAGAAGAAGCTATTGCTG TGACAATGAGGTTTCTACGTTCAGTGGCAAGAGTTTTTGTTATTCTGAGTGTGGAAATGGCTTCATCCAA AAAGAAAAACAACTTTATTCCACAGCCAATTGGAAAATGCAAGCGTGTATTCCAAGCATTGCTACCTTAC GCTGTGGAAGAATTGTGCAACGTAGCAGAGTCACTGATTGTTCCTGTCAGAATGGGGATTGCTCGTCCAA CTGCACCATTTACCCTGGCTAGTACTAGCATAGATGCCATGCAGGGCAGTGAAGAATTATTTTCAGTGGA ACCACTACCACCACGACCATCATCTGATCAGTCTAGCAGCTCCAGTCAGTCTCAGTCATCCTACATCATC AGGAATCCACAGCAGAGGCGCATCAGCCAGTCACAGCCCGTTCGGGGCAGAGATGAAGAACAGGATGATA TTGTTTCAGCAGATGTGGAAGAGGTTGAGGTGGTGGAGGGTGTGGCTGGAGAAGAGGATCATCATGATGA ACAGGAAGAACACGGGGAAGAAAATGCTGAGGCAGAGGGACAACATGATGAGCATGATGAAGACGGGAGT GATATGGAGCTGGACTTGTTAGCAGCAGCTGAAACAGAAAGTGATAGTGAAAGTAACCACAGCAACCAAG ATAATGCTAGTGGGCGCAGAAGCGTTGTCACTGCAGCAACTGCTGGTTCAGAAGCAGGAGCAAGCAGTGT TCCTGCCTTCTTTTCTGAAGATGATTCTCAATCGAATGACTCAAGTGATTCTGATAGCAGTAGTAGTCAG AGTGACGACATAGAACAGGAGACCTTTATGCTTGATGAGCCATTAGAAAGAACCACAAATAGCTCCCATG CCAATGGTGCTGCCCAAGCTCCCCGTTCAATGCAGTGGGCTGTCCGCAACACCCAGCATCAGCGAGCAGC CAGTACAGCCCCTTCCAGTACATCTACACCAGCAGCAAGTTCAGCGGGTTTGATTTATATTGATCCTTCA AACTTACGCCGGAGTGGTACCATCAGTACAAGTGCTGCAGCTGCAGCAGCTGCTTTGGAAGCTAGCAACG CCAGCAGTTACCTAACATCTGCAAGCAGTTTAGCCAGGGCTTACAGCATTGTCATTAGACAAATCTCGGA CTTGATGGGCCTTATTCCTAAGTATAATCACCTAGTATACTCTCAGATTCCAGCAGCTGTGAAATTGACT TACCAAGATGCAGTAAACTTACAGAACTATGTAGAAGAAAAGCTTATTCCCACTTGGAACTGGATGGTCA GTATTATGGATTCTACTGAAGCTCAATTACGTTATGGTTCTGCATTAGCATCTGCTGGTGATCCTGGACA TCCAAATCATCCTCTTCACGCTTCTCAGAATTCAGCGAGAAGAGAGAGGATGACTGCGCGAGAAGAAGCT AGCTTACGAACACTTGAAGGCAGACGACGTGCCACCTTGCTTAGCGCCCGTCAAGGAATGATGTCTGCAC GAGGAGACTTCCTAAATTATGCTCTGTCTCTAATGCGGTCTCATAATGATGAGCATTCTGATGTTCTTCC AGTTTTGGATGTTTGCTCATTGAAGCATGTGGCATATGTTTTTCAAGCACTTATATACTGGATTAAGGCA ATGAATCAGCAGACAACATTGGATACACCTCAACTAGAACGCAAAAGGACGCGAGAACTCTTGGAACTGG GTATTGATAATGAAGATTCAGAACATGAAAATGATGATGACACCAATCAAAGTGCTACTTTGAATGATAA GGATGATGACTCTCTTCCTGCAGAAACTGGCCAAAACCATCCATTTTTCCGACGTTCAGACTCCATGACA TTCCTTGGGTGTATACCCCCAAATCCATTTGAAGTGCCTCTGGCTGAAGCCATCCCCTTGGCTGATCAGC CACATCTGTTGCAGCCAAATGCTAGAAAGGAGGATCTTTTTGGCCGTCCAAGTCAGGGTCTTTATTCTTC ATCTGCCAGTAGTGGGAAATGTTTAATGGAGGTTACAGTGGATAGAAACTGCCTAGAGGTTCTTCCAACA AAAATGTCTTATGCTGCCAATCTGAAAAATGTAATGAACATGCAAAACCGGCAAAAAAAAGAAGGGGAAG AACAGCCCGTGCTGCCAGAAGAAACTGAGAGTTCAAAACCAGGGCCATCTGCTCATGATCTTGCTGCACA ATTAAAAAGTAGCTTACTAGCAGAAATAGGACTTACTGAAAGTGAAGGGCCACCTCTCACATCTTTCAGG CCACAGTGTAGCTTTATGGGAATGGTTATTTCCCATGATATGCTGCTAGGACGTTGGCGCCTTTCTTTAG AACTGTTCGGCAGGGTATTCATGGAAGATGTTGGAGCAGAACCTGGATCAATCCTAACTGAATTGGGTGG TTTTGAGGTAAAAGAATCAAAATTCCGCAGAGAAATGGAAAAACTGAGAAACCAGCAGTCAAGAGATTTG TCACTAGAGGTTGATCGGGATCGAGATCTTCTCATTCAGCAGACTATGAGGCAGCTTAACAATCACTTTG GTCGAAGATGTGCTACTACACCAATGGCTGTACACAGAGTAAAAGTCACATTTAAGGATGAGCCAGGAGA GGGCAGTGGTGTAGCACGAAGTTTTTATACAGCCATTGCACAAGCATTTTTATCAAATGAAAAATTGCCA AATCTAGAGTGTATCCAAAATGCCAACAAAGGCACCCACACAAGTTTAATGCAGAGATTAAGGAACCGAG GAGAGAGAGACCGGGAAAGGGAGAGAGAAAGGGAAATGAGGAGGAGTAGTGGTTTGCGAGCAGGTTCTCG GAGGGACCGGGATAGAGACTTTAGAAGACAGCTTTCCATCGACACTAGGCCCTTTAGACCAGCCTCTGAA GGGAATCCTAGCGATGATCCTGAGCCTTTGCCAGCACATCGGCAGGCACTTGGAGAGAGGCTTTATCCTC GTGTACAAGCAATGCAACCAGCATTTGCAAGTAAAATCACTGGCATGTTGTTGGAATTATCCCCAGCTCA GCTGCTTCTCCTTCTAGCAAGTGAGGATTCTCTGAGAGCAAGAGTGGATGAGGCCATGGAACTCATTATT GCACATGGACGGGAAAATGGAGCTGATAGTATCCTGGATCTTGGATTAGTAGACTCCTCAGAAAAGGTAC AGGAAAACCGAAAGCGCCATGGCTCTAGTCGAAGTGTAGTAGATATGGATTTAGATGATACAGATGATGG TGATGACAATGCCCCTTTGTTTTACCAACCTGGGAAAAGAGGATTTTATACTCCAAGGCCTGGCAAGAAC ACAGAAGCAAGGTTGAATTGTTTCAGAAACATTGGCAGGATTCTTGGACTATGTCTGTTACAGAATGAAC TATGTCCTATCACATTGAATAGACATGTAATTAAAGTATTGCTTGGTAGAAAAGTCAATTGGCATGATTT TGCTTTTTTTGATCCTGTAATGTATGAGAGTTTGCGGCAACTAATCCTCGCGTCTCAGAGTTCAGATGCT GATGCTGTTTTCTCAGCAATGGATTTGGCATTTGCAATTGACCTGTGTAAAGAAGAAGGTGGAGGACAGG TTGAACTCATTCCTAATGGTGTAAATATACCAGTCACTCCACAGAATGTATATGAGTATGTGCGGAAATA CGCAGAACACAGAATGTTGGTAGTTGCAGAACAGCCCTTACATGCAATGAGGAAAGGTCTACTAGATGTG CTTCCAAAAAATTCATTAGAAGATTTAACGGCAGAAGATTTTAGGCTTTTGGTAAATGGCTGCGGTGAAG TCAATGTGCAAATGCTGATCAGTTTTACCTCTTTCAATGATGAATCAGGAGAAAATGCTGAGAAGCTTCT GCAGTTCAAGCGTTGGTTCTGGTCAATAGTAGAGAAGATGAGCATGACAGAACGACAAGATCTTGTTTAC TTTTGGACATCAAGCCCATCACTGCCAGCCAGTGAAGAAGGATTCCAGCCTATGCCCTCAATCACAATAA GACCACCAGATGACCAACATCTTCCTACTGCAAATACTTGCATTTCTCGACTTTACGTCCCACTCTATTC CTCTAAACAGATTCTCAAACAGAAATTGTTACTCGCCATTAAGACCAAGAATTTTGGTTTTGTGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001282873
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282873.1, NP_001269802.1
RefSeq Size	10897 bp
RefSeq ORF	8397 bp
Locus ID	51366
UniProt ID	O95071
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.