PHF3 (NM_001290259) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290259, the custom clone sequence may differ by one or more nucleotides ATGGATGAAGGAGTTGTTAAAGAAAGTGGCAATGATACCATTGATGAAGAAGAACTGATTTTACCTAACA GGAACTTAAGGGACAAGGTAGAAGAAAATTCAGTGAGATCTCCAAGAAAATCACCTCGTTTAATGGCACA AGAACAAGTAAGAAGTTTGCGACAGAGCACTATTGCCAAGCGTTCAAATGCAGCACCATTAAGTAACACA AAAAAAGCATCTGGGAAGACTGTATCTACTGCTAAAGCAGGAGTGAAACAACCAGAAAGGAGTCAGGTTA AAGAAGAAGTATGTATGTCACTGAAACCTGAGTACCATAAGGAGAATAGAAGGTGCAGCCGAAATAGCGG ACAAATTGAAGTGGTACCTGAAGTATCAGTGTCTTCAAGTCATTCTTCAGTGTCATCTTGTCTTGAAATG AAGGATGAAGATGGATTAGATTCTAAGCATAAGTGTAATAATCCGGGAGAAATAGATGTGCCATCTCATG AATTAAATTGTTCACTTCTTTCAGAGACTTGTGTTACTATTGGAGAAAAGAAAAATGAAGCTTTGATGGA ATGTAAAGCCAAGCCTGTTGGTAGTCCATTGTTTAAGTTTTCAGATAAAGAAGAACATGAACAAAATGAT TCCATTTCAGGTAAAACGGGTGAGACTGTTGTTGAAGAAATGATAGCAACAAGAAAAGTTGAACAAGATT CAAAGGAGACAGTAAAATTATCCCATGAAGATGACCATATTCTTGAGGACGCTGGATCTTCTGATATTTC TAGTGATGCTGCTTGTACAAATCCAAATAAGACAGAAAACAGCCTTGTAGGTTTGCCTAGTTGTGTAGAT GAAGTGACTGAATGTAATTTGGAATTGAAGGATACCATGGGTATTGCTGATAAAACTGAGAACACCCTTG AAAGAAATAAAATTGAACCGTTGGGTTATTGTGAAGATGCGGAGTCTAATAGGCAGTTGGAGAGCACTGA GTTTAATAAATCAAACTTAGAGGTGGTTGATACTAGTACTTTTGGACCGGAAAGTAATATCTTGGAAAAT GCTATTTGTGATGTGCCTGACCAAAATTCAAAACAGTTGAATGCTATAGAAAGTACTAAAATAGAGTCCC ATGAAACAGCAAACCTTCAGGATGACAGAAACAGCCAGTCAAGTAGCGTTTCTTACTTAGAGTCAAAAAG TGTAAAATCCAAACATACAAAACCTGTAATTCATTCTAAGCAAAACATGACCACAGATGCTCCGAAGAAA ATTGTTGCAGCAAAGTATGAAGTAATACATAGCAAAACTAAAGTTAATGTCAAAAGTGTGAAACGAAATA CTGATGTACCAGAATCTCAGCAAAATTTTCATAGGCCAGTCAAAGTCAGAAAAAAACAAATTGATAAGGA GCCAAAGATTCAGAGTTGCAATTCTGGGGTTAAATCTGTGAAAAACCAAGCTCATTCTGTACTGAAAAAA ACATTACAGGATCAAACTTTAGTACAAATTTTCAAGCCCTTAACTCATTCTTTGAGTGATAAGTCACACG CTCATCCTGGTTGCTTGAAAGAACCTCATCATCCTGCACAAACTGGACATGTATCACATTCTAGCCAGAA ACAGTGTCATAAGCCTCAGCAACAGGCCCCAGCAATGAAAACCAATAGTCACGTGAAGGAAGAGCTTGAA CACCCAGGCGTTGAGCATTTTAAGGAAGAGGATAAACTGAAACTGAAAAAACCTGAGAAGAACCTACAAC CCCGCCAAAGAAGAAGCAGCAAAAGTTTTTCTTTAGATGAGCCACCATTGTTCATTCCAGATAACATAGC TACCATAAGAAGAGAAGGCTCTGATCATAGCTCCTCATTTGAAAGCAAATATATGTGGACTCCCAGCAAG CAGTGTGGGTTTTGCAAAAAACCACATGGCAACAGGTTTATGGTTGGCTGTGGGAGATGTGATGACTGGT TTCATGGTGATTGTGTTGGGTTAAGTCTTTCTCAAGCACAGCAGATGGGCGAGGAAGACAAAGAATATGT CTGTGTAAAATGTTGTGCTGAAGAAGACAAAAAGACTGAAATACTAGATCCAGATACTTTGGAAAACCAA GCTACAGTTGAATTCCATAGTGGAGATAAAACAATGGAGTGTGAAAAGCTTGGATTATCAAAACACACAA CAAATGATAGAACCAAATATATAGATGATACAGTGAAGCACAAGGTCAAAATTTTAAAACGGGAGTCTGG TGAAGGCAGAAATTCATCAGACTGTAGAGATAATGAAATTAAAAAATGGCAGCTAGCTCCTCTTCGTAAG ATGGGACAACCAGTTTTACCTCGGAGATCCTCAGAAGAAAAAAGTGAAAAAATACCGAAAGAGTCTACAA CTGTTACTTGCACAGGAGAAAAAGCTTCAAAACCAGGTACTCATGAGAAGCAAGAGATGAAAAAGAAGAA AGTTGAAAAAGGAGTGCTTAATGTACATCCTGCTGCTTCTGCTTCCAAGCCTTCTGCAGATCAGATCAGG CAAAGTGTCAGACATTCTCTCAAAGACATTCTTATGAAGAGACTTACAGACTCAAATTTGAAGGTACCAG AGGAAAAGGCAGCAAAAGTTGCCACAAAAATTGAGAAAGAGCTTTTCTCTTTTTTTCGGGACACAGATGC TAAATATAAGAACAAATATAGAAGTTTGATGTTTAATTTGAAAGATCCTAAAAACAATATATTATTTAAA AAAGTACTGAAAGGAGAAGTAACTCCTGATCATCTTATCAGAATGAGTCCAGAAGAACTAGCTTCTAAAG AGTTAGCTGCTTGGAGACGAAGAGAAAACAGACATACCATAGAAATGATTGAGAAAGAGCAGAGAGAAGT GGAACGACGGCCAATCACCAAAATAACTCATAAAGGTGAAATAGAAATTGAGAGTGATGCCCCAATGAAA GAACAGGAAGCAGCCATGGAGATTCAGGAACCAGCCGCCAATAAGTCATTGGAGAAGCCAGAAGGATCTG AAAAACAAAAAGAGGAGGTTGACTCTATGTCTAAAGATACCACTAGTCAACACAGACAGCATCTTTTTGA TCTCAACTGCAAAATCTGCATAGGTCGAATGGCACCACCTGTAGATGATCTTTCTCCAAAAAAAGTAAAA GTTGTTGTAGGAGTAGCTCGCAAACATTCAGACAATGAAGCAGAAAGTATAGCAGATGCATTATCTTCAA CCTCAAATATTTTGGCTTCTGAATTCTTTGAGGAGGAGAAACAGGAGTCTCCAAAGTCAACGTTCTCTCC TGCTCCACGTCCAGAGATGCCTGGAACTGTTGAAGTTGAGTCTACCTTTCTGGCTCGATTGAACTTCATC TGGAAAGGTTTTATCAACATGCCTTCTGTGGCAAAATTTGTTACCAAAGCCTATCCAGTATCTGGCTCCC CAGAATACCTGACAGAGGACCTACCAGATAGTATTCAAGTAGGTGGCAGGATATCACCTCAGACAGTTTG GGATTATGTGGAAAAAATAAAAGCATCAGGAACCAAGGAAATTTGTGTGGTTCGCTTCACACCAGTAACT GAAGAAGATCAAATTTCTTATACTTTGCTCTTTGCATACTTCAGTAGCAGAAAGCGCTATGGAGTAGCTG CTAACAACATGAAGCAGGTTAAAGATATGTACCTTATTCCTTTGGGTGCCACAGATAAAATTCCACACCC TCTTGTGCCTTTTGATGGACCTGGGCTTGAACTGCATAGACCTAATCTATTGTTGGGCTTAATTATTCGT CAGAAACTGAAGCGACAGCACAGTGCCTGTGCTAGTACTAGTCATATAGCTGAGACTCCTGAAAGTGCAC CACCAATAGCATTGCCACCTGATAAAAAAAGTAAAATAGAAGTTTCTACAGAAGAAGCACCAGAGGAAGA AAATGACTTTTTTAATTCTTTTACAACTGTATTACACAAGCAGAGAAATAAACCTCAGCAGAATCTTCAG GAAGACCTTCCAACAGCAGTTGAACCTTTAATGGAAGTCACCAAACAGGAGCCACCAAAACCTTTAAGAT TTCTTCCTGGCGTGTTGATTGGCTGGGAGAATCAACCTACTACTCTGGAATTAGCAAATAAACCTCTTCC TGTGGATGATATACTTCAAAGCCTTTTGGGCACCACTGGTCAAGTATATGACCAGGCCCAGTCAGTGATG GAACAAAACACTGTTAAAGAAATTCCATTTTTAAATGAGCAGACCAACTCAAAAATAGAGAAAACAGATA ATGTGGAAGTAACTGATGGTGAAAACAAGGAGATAAAAGTTAAAGTAGATAATATTTCAGAATCTACAGA TAAGTCAGCAGAAATAGAAACATCAGTAGTAGGGTCCTCTTCCATTTCTGCAGGGTCTTTGACGAGTCTT AGTCTCAGAGGTAAGCCACCAGATGTTTCTACAGAAGCATTTTTAACAAATTTATCAATTCAGTCAAAAC AAGAGGAAACTGTGGAGAGTAAAGAGAAAACATTAAAAAGACAGCTTCAGGAAGATCAAGAGAATAATTT GCAAGATAACCAGACTTCAAATAGTTCTCCATGCAGATCTAATGTAGGAAAAGGAAACATAGATGGTAAT GTGAGCTGTAGTGAAAACCTTGTTGCTAATACAGCGAGGTCTCCACAGTTTATCAACCTGAAAAGGGATC CTAGGCAAGCAGCAGGACGAAGTCAGCCTGTAACTACTTCAGAAAGCAAAGATGGAGATAGTTGCCGGAA TGGAGAAAAACACATGCTGCCTGGCCTGTCACACAACAAGGAGCACTTAACAGAACAAATCAATGTAGAG GAAAAGTTGTGTTCTGCAGAGAAAAACTCGTGTGTTCAGCAGAGTGACAATTTAAAAGTTGCACAAAACT CACCATCAGTAGAAAACATACAGACTTCTCAAGCAGAACAAGCAAAACCCTTACAGGAGGATATTTTAAT GCAAAATATTGAAACTGTGCACCCATTTCGAAGAGGATCAGCAGTAGCGACATCTCATTTTGAAGTTGGA AACACATGTCCATCAGAATTTCCTTCTAAAAGCATCACCTTTACTTCCAGAAGCACCAGCCCCAGAACAA GTACAAACTTTTCACCCATGAGGCCACAGCAGCCCAACCTTCAGCATCTCAAGTCTAGCCCACCTGGATT TCCATTTCCAGGGCCTCCTAATTTTCCCCCACAAAGCATGTTTGGATTTCCACCACATTTGCCACCTCCA TTACTTCCCCCTCCAGGCTTTGGCTTTGCTCAAAATCCCATGGTTCCCTGGCCACCTGTTGTTCATCTCC CAGGTCAGCCACAGCGTATGATGGGTCCTCTCTCACAAGCATCAAGGTATATAGGCCCGCAGAATTTTTA CCAGGTTAAAGACATTCGGAGGCCAGAAAGGCGCCATAGTGACCCTTGGGGTAGGCAAGACCAACAGCAA CTGGATAGGCCATTTAATAGGGGTAAAGGGGACCGCCAGAGATTTTATAGTGATTCACACCATTTGAAAA GAGAGCGACATGAAAAGGAATGGGAGCAAGAATCTGAAAGGCATAGACGCAGAGACAGAAGCCAAGACAA GGACAGAGACAGAAAAAGCAGGGAGGAAGGGCACAAAGATAAAGAGAGGGCACGGTTATCACATGGTGAT CGAGGAACAGATGGAAAAGCAAGCAGAGATAGTAGGAATGTAGACAAGAAGCCAGATAAACCTAAAAGTG AAGACTATGAGAAGGACAAAGAACGAGAGAAAAGTAAACACAGAGAAGGAGAAAAGGACAGGGATAGGTA CCACAAAGATAGGGACCACACTGACAGAACTAAAAGCAAAAGGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290259
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290259.1, NP_001277188.1
RefSeq Size	8501 bp
RefSeq ORF	5856 bp
Locus ID	23469
UniProt ID	Q92576
Protein Families	Transcription Factors
Gene Summary	This gene encodes a member of a PHD finger-containing gene family. This gene may function as a transcription factor and may be involved in glioblastomas development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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