DOCK1 (NM_001290223) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ced5; DOCK180
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290223, the custom clone sequence may differ by one or more nucleotides ATGACGCGCTGGGTGCCCACCAAGCGCGAGGAGAAGTACGGCGTGGCTTTTTATAACTATGATGCCAGAG GAGCGGATGAACTTTCTTTACAGATCGGAGACACTGTGCACATCTTAGAAACATATGAAGGGTGGTACCG AGGTTACACGTTACGAAAAAAGTCTAAGAAGGGTATATTTCCTGCTTCATATATTCATCTTAAAGAAGCG ATAGTTGAAGGAAAAGGGCAACATGAAACAGTCATCCCGGGTGACCTCCCCCTCATCCAGGAAGTCACCA CGACACTCCGAGAGTGGTCCACCATCTGGAGGCAGCTCTACGTGCAAGATAACAGGGAGATGTTTCGAAG TGTGCGGCACATGATCTATGACCTTATTGAATGGCGATCACAAATTCTTTCTGGAACTCTGCCTCAGGAT GAACTCAAAGAACTGAAGAAGAAGGTCACAGCCAAAATTGATTATGGAAACAGAATTCTAGATTTGGACC TGGTGGTTAGAGATGAAGATGGGAATATTTTGGATCCAGAATTAACTAGCACGATTAGTCTCTTCAGAGC TCATGAAATAGCTTCTAAACAAGTGGAGGAAAGGTTACAAGAGGAAAAATCTCAAAAGCAGAACATAGAT ATTAACAGACAAGCCAAGTTTGCTGCAACCCCTTCTCTGGCCTTGTTTGTGAACCTCAAAAATGTGGTTT GTAAAATAGGAGAAGATGCTGAAGTCCTCATGTCTCTATATGACCCTGTGGAGTCCAAATTCATCAGTGA GAACTACCTGGTTCGCTGGTCCAGTTCAGGATTACCTAAAGACATAGACAGATTACATAATTTGCGAGCC GTGTTTACTGACCTCGGAAGCAAAGACCTGAAAAGGGAGAAAATCAGTTTTGTCTGTCAGATTGTTCGCG TGGGTCGCATGGAGCTGAGGGACAACAACACCAGGAAACTGACCTCGGGGTTGCGGCGACCTTTTGGAGT GGCTGTGATGGATGTAACAGATATAATAAATGGAAAAGTAGATGATGAAGATAAGCAGCATTTCATTCCC TTTCAGCCGCTCGCGTTGGACGACGCCATTCGACACAAGCCGCTGAACATGTCATCCCGTTTTTCACCCA GGGTGGCAGGGGAGAATGACTTCCTTCAGACTGTTATAAACAAAGTCATCGCTGCCAAAGAAGTCAACCA CAAGGGGCAGGGTTTGTGGGTAACATTGAAATTACTTCCTGGAGATATCCATCAGATCCGAAAAGAGTTT CCGCATTTAGTGGACAGGACCACAGCTGTGGCTCGAAAAACAGGGTTTCCGGAGATAATCATGCCTGGTG ATGTTCGAAATGATATCTATGTAACATTAGTTCAAGGAGATTTTGATAAAGGAAGCAAAACAACAGCGAA GAACGTGGAGGTCACGGTGTCTGTGTACGATGAGGATGGGAAACGATTAGAGCATGTGATTTTCCCGGGT GCTGGTGATGAAGCGATTTCAGAGTACAAATCTGTGATTTACTACCAAGTAAAGCAGCCACGCTGGTTTG AGACTGTTAAGGTGGCCATTCCCATCGAGGACGTTAACCGCAGTCACCTTCGGTTTACCTTCCGCCACAG GTCATCACAGGACTCTAAGGATAAATCTGAGAAAATATTTGCACTAGCATTTGTCAAGCTGATGAGATAC GATGGTACCACCCTGCGAGACGGAGAGCACGATCTTATCGTCTATAAGGCCGAAGCAAAGAAGCTGGAAG ATGCTGCCACGTACTTGAGTCTGCCCTCCACGAAGGCAGAGTTGGAAGAAAAGGGCCACTCGGCCACCGG CAAGAGCATGCAGAGCCTTGGGAGCTGCACCATTAGCAAGGACTCCTTCCAGATCTCCACGCTCGTGTGC TCCACCAAACTGACTCAGAACGTGGACCTTCTGGGGCTCTTGAAATGGCGCTCCAACACCAGCCTGCTGC AGCAGAACTTGAGGCAGCTGATGAAAGTCGATGGTGGTGAAGTAGTGAAGTTTCTTCAGGACACGTTGGA TGCCCTCTTCAACATCATGATGGAGAACTCAGAGAGTGAGACTTTTGACACGTTAGTCTTTGATGCTCTG GTATTTATCATTGGACTGATTGCTGATAGAAAATTTCAGCATTTTAATCCTGTTTTGGAAACTTACATTA AGAAACACTTTAGTGCAACGTTAGCCTACACGAAGTTGACAAAAGTGTTGAAGAACTACGTGGACGGTGC TGAGAAGCCGGGAGTAAATGAGCAGCTGTACAAAGCCATGAAAGCGCTAGAATCCATCTTCAAGTTCATC GTGCGCTCCAGGATCCTGTTCAATCAACTGTATGAAAACAAGGGAGAGGCTGACTTCGTGGAATCTTTGC TGCAGCTCTTCAGGTCCATCAATGACATGATGAGCAGCATGTCAGACCAGACCGTCCGGGTGAAGGGGGC AGCACTGAAATACTTACCAACGATCGTCAACGATGTGAAATTGGTGTTTGATCCCAAAGAGCTCAGCAAA ATGTTTACTGAATTCATCCTCAATGTTCCCATGGGCTTGCTGACCATCCAGAAACTCTACTGCTTGATCG AAATCGTCCACAGTGACCTCTTCACACAGCATGACTGCAGAGAGATCCTGCTTCCCATGATGACCGATCA GCTCAAGTACCATCTGGAGAGACAGGAGGACCTGGAGGCCTGCTGTCAGCTGCTCAGCCACATCCTGGAG GTGCTGTACAGGAAGGACGTGGGGCCAACCCAGAGGCACGTCCAGATTATCATGGAGAAACTTCTCCGGA CCGTGAACCGAACCGTCATTTCCATGGGACGAGATTCTGAACTCATTGGAAACTTCGTGGCTTGCATGAC AGCTATTTTACGACAAATGGAAGATTACCATTATGCCCACTTGATCAAGACTTTTGGGAAAATGAGGACT GATGTGGTAGATTTCCTAATGGAAACATTCATCATGTTTAAGAACCTCATTGGAAAGAACGTTTACCCCT TCGACTGGGTGATCATGAACATGGTGCAAAATAAAGTCTTCCTGCGAGCAATTAATCAGTATGCAGATAT GCTGAACAAAAAATTTCTGGATCAAGCCAACTTTGAGCTACAGCTGTGGAACAACTACTTTCACCTGGCT GTTGCTTTCCTTACTCAAGAGTCCCTGCAACTGGAGAATTTTTCAAGTGCCAAGAGAGCCAAAATCCTTA ACAAGTACGGAGATATGAGGAGACAGATTGGCTTTGAAATCAGAGACATGTGGTACAACCTTGGTCAACA CAAGATAAAGTTCATTCCAGAAATGGTGGGCCCAATATTAGAAATGACATTAATTCCCGAGACGGAGCTG CGCAAAGCCACCATCCCCATCTTCTTTGATATGATGCAGTGTGAATTCCATTCGACCCGAAGCTTCCAAA TGTTTGAAAATGAGATCATCACCAAGCTGGATCATGAAGTCGAAGGAGGCAGAGGAGACGAACAGTACAA AGTGTTATTTGATAAAATCCTTCTGGAACACTGCAGGAAGCACAAATACCTCGCCAAAACAGGAGAAACT TTTGTAAAACTCGTTGTGCGCTTAATGGAAAGGCTTTTGGATTATAGAACCATCATGCACGACGAGAACA AAGAAAACCGCATGAGCTGCACCGTCAATGTGCTGAATTTCTACAAAGAAATTGAAAGAGAAGAAATGTA TATAAGGTATTTGTACAAGCTCTGTGACCTGCACAAGGAGTGTGATAACTACACCGAAGCGGCTTACACC TTGCTTCTCCATGCAAAGCTTCTTAAGTGGTCGGAGGATGTGTGTGTGGCCCACCTCACCCAGCGGGACG GGTACCAGGCCACCACGCAGGGACAGCTGAAGGAGCAGCTCTACCAGGAAATCATCCACTACTTCGACAA AGGCAAGATGTGGGAGGAGGCCATTGCCTTGGGCAAGGAGCTAGCCGAGCAGTATGAGAACGAAATGTTT GATTATGAGCAACTCAGCGAATTGCTGAAAAAACAGGCTCAGTTTTATGAAAACATCGTCAAAGTGATCA GGCCCAAGCCTGACTATTTTGCTGTTGGCTACTACGGACAAGGGTTCCCCACATTCCTGCGGGGAAAAGT TTTCATTTACCGAGGGAAAGAGTATGAGCGCCGGGAAGATTTTGAGGCTCGGCTCTTAACTCAGTTTCCA AACGCCGAGAAAATGAAGACAACATCTCCACCAGGCGACGATATTAAAAACTCTCCTGGCCAGTATATTC AGTGCTTCACAGTGAAGCCCAAACTCGATCTGCCTCCTAAGTTTCACAGGCCAGTGTCAGAGCAGATTGT AAGTTTTTACAGGGTGAACGAGGTCCAGCGATTTGAATATTCTCGGCCAATCCGGAAGGGAGAGAAAAAC CCAGACAATGAATTTGCGAATATGTGGATCGAGAGAACCATATATACAACTGCATATAAATTACCTGGAA TTTTAAGGTGGTTTGAGGTCAAGTCTGTTTTCATGGTGGAAATCAGCCCCCTGGAGAATGCCATTGAGAC CATGCAGCTGACGAACGACAAGATCAACAGCATGGTGCAGCAGCACCTGGATGACCCCAGCCTGCCCATC AACCCGCTCTCCATGCTCCTGAACGGCATCGTGGACCCAGCTGTCATGGGGGGCTTCGCAAACTACGAAA AGGCCTTCTTTACAGACCGGTACCTGCAGGAGCACCCTGAGGCCCATGAAAAGATCGAGAAGCTCAAGGA CCTGATTGCTTGGCAGATTCCTTTTCTGGCCGAAGGGATCAGAATCCATGGAGACAAAGTCACGGAGGCA CTGAGGCCGTTCCACGAGAGGATGGAGGCCTGTTTCAAACAGCTGAAGGAAAAGGTGGAGAAAGAGTACG GCGTCCGAATCATGCCCTCAAGTCTGGATGATAGAAGAGGCAGCCGCCCCCGGTCCATGGTGCGGTCCTT CACGATGCCTTCCTCATCCCGCCCTCTGTCTGTGGCCTCTGTCTCTTCCCTCTCATCGGACAGCACCCCT TCCAGACCAGGCTCCGACGGGTTTGCCCTGGAGCCTCTCCTGCCAAAGAAAATGCACTCCAGGTCCCAGG ACAAGCTGGACAAGGATGACCTGGAGAAGGAGAAGAAGGACAAGAAGAAGGAAAAAAGGAACAGCAAACA TCAAGAGATATTTGAGAAAGAATTTAAACCCACCGACATTTCCCTGCAGCAGTCTGAGGCTGTGATCCTT TCGGAAACGATAAGTCCCCTGCGGCCCCAGAGACCGAAGAGCCAGGTGATGAACGTCATTGGAAGCGAAA GGCGCTTCTCGGTGTCCCCCTCGTCACCGTCCTCCCAGCAAACACCCCCTCCAGTTACACCAAGGGCCAA GCTCAGCTTCAGCATGCAGTCGAGCTTGGAGCTGAACGGCATGACGGGGGCGGACGTGGCCGATGTCCCA CCCCCTCTGCCTCTCAAAGGCAGCGTGGCAGATTACGGGAATTTGATGGAAAACCAGGACTTGCTGGGCT CGCCAACACCTCCACCTCCCCCTCCACACCAGAGGCATCTGCCACCTCCACTGCCCAGCAAAACTCCGCC TCCTCCCCCTCCAAAGACAACTCGCAAGCAGACATCGGTGGACTCCGGGATCGTGCAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001290223
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290223.1, NP_001277152.1
RefSeq Size	6876 bp
RefSeq ORF	5661 bp
Locus ID	1793
Protein Families	Druggable Genome
Protein Pathways	Focal adhesion, Regulation of actin cytoskeleton
Gene Summary	This gene encodes a member of the dedicator of cytokinesis protein family. Dedicator of cytokinesis proteins act as guanine nucleotide exchange factors for small Rho family G proteins. The encoded protein regulates the small GTPase Rac, thereby influencing several biological processes, including phagocytosis and cell migration. Overexpression of this gene has also been associated with certain cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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