PPRC1 (NM_001288728) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PRC
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288728, the custom clone sequence may differ by one or more nucleotides ATGAGACACTGCTGGGGACCATGCAGAGCTACATGGATGCCTCCCTTATCTCCCTCATTGAGGATTTTGG GAGCCTTGGAGAGCATTCCTGATTCGGAGCTGCTTGTGTCACCCCGGGAGGGCTCCTCTCTGCACAAGCT GCTTACTCTCTCTCGGACACCCCCAGAACGTGACCTCATCACCCCAGTTGACCCACTGGGGCCCAGTACA GGCAGCAGTAGAGGGAGTGGGGTTGAAATGTCTCTTCCAGATCCCTCTTGGGACTTCTCCCCACCCTCTT TCTTAGAGACCTCTTCCCCCAAGCTTCCTAGCTGGAGACCCCCAAGATCAAGACCACGCTGGGGCCAATC CCCACCTCCCCAGCAGCGCAGTGATGGAGAAGAAGAGGAGGAGGTGGCCAGCTTCAGTGGCCAGATTCTT GCCGGGGAGCTTGACAACTGTGTGAGCAGTATCCCGGACTTCCCCATGCATTTGGCCTGCCCTGAGGAGG AAGATAAAGCAACAGCAGCAGAGATGGCAGTGCCAGCAGCTGGTGATGAGAGCATCTCCTCCCTGAGTGA GCTGGTGCGGGCCATGCACCCATACTGCCTGCCCAACCTCACCCACCTGGCATCACTTGAGGATGAGCTT CAGGAGCAGCCAGATGATTTGACACTGCCTGAGGGCTGCGTAGTGCTGGAGATTGTGGGGCAGGCAGCCA CAGCTGGCGATGACCTGGAGATCCCAGTTGTGGTGCGACAGGTCTCTCCTGGACCCCGGCCTGTGCTCCT GGATGACTCGCTAGAGACTAGTTCTGCCTTGCAGCTGCTTATGCCTACACTGGAGTCAGAGACAGAGGCT GCTGTGCCCAAGGTAACCCTCTGCTCTGAGAAAGAGGGGTTGTCATTGAACTCAGAGGAGAAGCTGGACT CAGCCTGCTTATTGAAGCCCAGGGAGGTCGTGGAGCCGGTGGTGCCCAAGGAGCCTCAGAACCCACCTGC CAATGCAGCACCAGGTTCCCAGAGAGCTCGAAAGGGCAGGAAGAAGAAGAGCAAGGAGCAGCCAGCAGCC TGTGTGGAAGGCTATGCCAGGAGGCTGAGGTCATCTTCTCGCGGGCAGTCTACTGTAGGTACAGAAGTGA CCTCTCAGGTAGACAACTTGCAGAAACAGCCTCAGGAAGAACTTCAAAAAGAGTCTGGGCCTCTCCAGGG TAAGGGGAAGCCCCGGGCTTGGGCTCGGGCCTGGGCAGCTGCCTTGGAGAATTCTAGCCCTAAGAACTTG GAGAGAAGTGCTGGACAAAGTAGTCCTGCTAAAGAAGGCCCTCTAGACCTCTACCCAAAGCTGGCTGACA CTATCCAAACCAATCCTATACCAACCCATCTCTCATTGGTCGACTCTGCCCAAGCCAGCCCCATGCCAGT TGACTCTGTTGAAGCTGATCCCACTGCAGTTGGCCCTGTTCTAGCTGGCCCTGTACCTGTTGACCCTGGG TTGGTTGACCTTGCTTCAACCAGCTCAGAACTGGTTGAGCCTCTCCCGGCTGAGCCAGTGCTGATCAACC CAGTCCTGGCTGACTCAGCAGCAGTTGACCCTGCAGTGGTTCCCATCTCAGATAACTTGCCACCAGTTGA TGCTGTCCCGTCTGGCCCAGCACCAGTTGATCTAGCACTGGTTGACCCTGTTCCTAATGACCTGACTCCA GTTGACCCAGTGCTAGTTAAGTCCAGACCAACTGATCCCAGACGTGGTGCAGTGTCATCAGCCCTGGGGG GTTCAGCACCCCAGCTCCTCGTGGAGTCAGAGTCCTTGGACCCACCAAAGACCATCATCCCTGAAGTCAA AGAGGTTGTGGATTCTCTGAAAATTGAAAGTGGTACCAGTGCTACAACCCATGAAGCCAGACCTCGGCCT CTCAGCTTATCTGAGTACCGGCGACGAAGGCAGCAACGCCAAGCAGAAACAGAAGAGAGAAGTCCACAGC CCCCAACTGGGAAGTGGCCTAGCCTTCCAGAGACTCCCACAGGGCTGGCAGACATCCCTTGTCTTGTCAT CCCACCAGCCCCAGCCAAGAAGACAGCTCTGCAGAGAAGCCCTGAAACACCCCTTGAGATTTGCCTTGTG CCTGTAGGTCCCAGCCCTGCTTCTCCTAGTCCTGAGCCACCTGTAAGCAAACCTGTGGCCTCATCTCCCA CTGAGCAGGTGCCATCCCAGGAGATGCCACTGTTGGCGAGACCTTCCCCTCCTGTGCAGTCTGTGTCCCC TGCTGTGCCCACACCTCCCTCGATGTCTGCTGCCCTGCCTTTCCCTGCAGGTGGGCTTGGCATGCCCCCC AGTCTGCCCCCACCTCCCTTGCAGCCTCCTAGTCTTCCATTGTCTATGGGGCCAGTACTACCTGATCCGT TTACTCACTATGCCCCCTTGCCATCCTGGCCTTGTTATCCTCATGTGTCCCCTTCTGGCTATCCTTGCCT GCCCCCCCCACCAACGGTGCCCCTAGTGTCTGGTACTCCTGGTGCCTATGCCGTGCCTCCCACTTGCAGT GTGCCTTGGGCACCCCCTCCTGCCCCAGTCTCACCTTACAGTTCCACATGTACCTATGGGCCCTTGGGAT GGGGCCCAGGGCCTCAACATGCTCCATTCTGGTCTACTGTTCCCCCACCTCCTTTGCCTCCAGCCTCCAT TGGGAGAGCTGTTCCCCAACCTAAAATGGAGTCTAGGGGCACTCCAGCTGGCCCTCCTGAAAATGTACTT CCCTTGTCGATGGCTCCTCCCCTCAGTCTTGGGCTACCTGGCCATGGAGCTCCTCAGACAGAGCCTACCA AGGTGGAGGTCAAGCCAGTGCCTGCATCTCCCCATCCGAAACACAAGGTGTCTGCCCTGGTGCAAAGTCC CCAGATGAAGGCTCTAGCATGTGTGTCTGCTGAAGGTGTGACTGTTGAGGAGCCTGCATCAGAGAGGCTA AAGCCTGAGACCCAAGAGACCAGGCCCAGGGAGAAGCCCCCCTTGCCTGCTACCAAGGCTGTTCCCACAC CAAGGCAGAGCACTGTCCCCAAGCTGCCTGCTGTCCACCCAGCCCGTCTAAGGAAGCTGTCCTTCCTGCC TACCCCACGTACTCAGGGTTCTGAAGATGTGGTACAGGCTTTCATCAGTGAGATTGGAATTGAGGCATCG GACCTGTCCAGTCTGCTGGAGCAGTTTGAGAAATCAGAAGCCAAAAAGGAGTGTCCTCCTCCGGCTCCTG CTGACAGCTTGGCTGTAGGAAACTCAGGCGGCGTTGACATTCCCCAGGAGAAGAGGCCCCTAGACCGGTT ACAAGCCCCAGAACTGGCCAACGTGGCAGGGCTCACCCCTCCAGCTACCCCTCCCCACCAGTTATGGAAG CCCCTGGCTGCTGTCTCACTGCTGGCCAAAGCCAAATCTCCTAAGTCCACCGCCCAGGAGGGAACCCTGA AGCCTGAAGGAGTTACGGAGGCCAAACATCCAGCTGCAGTTCGCCTCCAAGAAGGGGTCCATGGCCCTAG TCGAGTCCATGTGGGCTCTGGGGACCATGACTATTGTGTCCGGAGCAGGACCCCCCCAAAAAAGATGCCT GCCCTAGTCATTCCAGAGGTGGGCTCCCGATGGAATGTCAAGCGCCATCAGGACATCACCATCAAACCTG TCTTGTCCTTGGGCCCAGCTGCCCCTCCGCCCCCATGCATAGCTGCCTCCCGGGAGCCGCTTGATCACAG GACTAGCAGTGAGCAGGCAGATCCCTCAGCACCCTGCCTTGCCCCATCCAGCTTGCTGTCCCCTGAGGCC TCACCCTGCCGGAATGACATGAACACTAGGACTCCCCCTGAACCCTCAGCCAAGCAGCGGTCAATGCGCT GTTACCGAAAAGCCTGCAGGTCAGCCAGCCCCTCAAGCCAGGGCTGGCAGGGCCGCCGAGGCCGCAACAG CCGTTCTGTCAGCTCTGGGTCCAACCGGACTAGCGAAGCATCTTCCTCATCCTCATCATCGTCTTCCTCA TCCCGATCTCGGTCCAGGTCCCTCTCCCCCCCACACAAGAGGTGGCGAAGGTCCAGCTGTAGTTCCTCTG GACGTTCTCGAAGATGCTCTTCCTCTTCTTCGTCATCATCTTCCTCTTCGTCTTCCTCATCCTCATCATC CAGTTCTCGAAGCCGCTCACGATCCCCATCCCCCCGCCGGAGAAGTGACAGGAGGCGGCGCTCTTATCGT TCACATGACCATTACCAAAGGCAAAGAGTGCTACAAAAGGAGCGTGCAATAGAAGAAAGAAGGGTGGTCT TCATTGGAAAGATACCTGGCCGCATGACTCGATCAGAGCTGAAACAGAGGTTCTCCGTTTTTGGAGAGAT TGAGGAGTGCACCATCCACTTCCGTGTCCAAGGGGACAACTACGGCTTCGTCACTTATCGCTATGCTGAG GAGGCATTTGCAGCCATTGAGAGTGGCCACAAGCTGCGGCAGGCAGATGAGCAGCCCTTTGATCTCTGCT TTGGGGGCCGAAGGCAGTTCTGCAAGAGGAGCTATTCTGATCTTGACTCCAACCGGGAAGACTTTGACCC AGCACCTGTAAAGAGCAAATTTGATTCTCTTGACTTTGACACATTGTTGAAACAGGCCCAGAAGAACCTC AGGAGGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001288728
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288728.1, NP_001275657.1
RefSeq Size	5283 bp
RefSeq ORF	4629 bp
Locus ID	23082
UniProt ID	Q5VV67
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene is similar to PPAR-gamma coactivator 1 (PPARGC1/PGC-1), a protein that can activate mitochondrial biogenesis in part through a direct interaction with nuclear respiratory factor 1 (NRF1). This protein has been shown to interact with NRF1. It is thought to be a functional relative of PPAR-gamma coactivator 1 that activates mitochondrial biogenesis through NRF1 in response to proliferative signals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, which leads to a frameshift, compared to variant 1. The encoded isoform (3) has a shorter and distinct N-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.