USP47 (NM_001282659) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TRFP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282659, the custom clone sequence may differ by one or more nucleotides ATGGTGCCCGGCGAGGAGAACCAACTGGTCCCGAAAGAGATAGAAAATGCTGCTGAAGAACCTAGAGTCT TATGTATTATACAAGATACTACTAATTCAAAGACAGTGAATGAACGGATCACTTTAAATTTACCAGCATC TACTCCAGTCAGAAAGCTCTTTGAAGATGTGGCCAACAAAGTAGGCTACATAAATGGAACCTTTGACTTG GTGTGGGGAAATGGAATCAATACTGCTGATATGGCACCACTGGATCATACCAGTGACAAGTCACTTCTCG ACGCTAATTTTGAGCCAGGAAAGAAGAACTTTCTGCATTTGACAGATAAAGATGGTGAACAACCTCAAAT ACTGCTGGAGGATTCCAGTGCTGGGGAAGACAGTGTTCATGACAGGTTTATAGGTCCGCTTCCAAGAGAA GGTTCTGGGGGTTCTACCAGTGATTATGTCAGCCAAAGCTACTCCTACTCATCTATTTTGAATAAATCAG AAACTGGATATGTGGGACTAGTAAACCAAGCAATGACTTGCTATTTGAATAGCCTTTTGCAAACACTTTT TATGACTCCTGAATTTAGGAATGCATTATATAAGTGGGAATTTGAAGAATCTGAAGAAGATCCAGTGACA AGTATTCCATACCAACTTCAAAGGCTTTTTGTTTTGTTACAAACCAGCAAAAAGAGAGCAATTGAAACCA CAGATGTTACAAGGAGCTTTGGATGGGATAGTAGTGAGGCTTGGCAGCAGCATGATGTACAAGAACTATG CAGAGTCATGTTTGATGCTTTGGAACAGAAATGGAAGCAAACAGAACAGGCTGATCTTATAAATGAGCTA TATCAAGGCAAGCTGAAGGACTACGTGAGATGTCTGGAATGTGGTTATGAGGGCTGGCGAATCGACACAT ATCTTGATATTCCATTGGTCATCCGACCTTATGGGTCCAGCCAAGCATTTGCTAGTGTGGAAGAAGCATT GCATGCATTTATTCAGCCAGAGATTCTGGATGGCCCAAATCAGTATTTTTGTGAACGTTGTAAGAAGAAG TGTGATGCACGGAAGGGCCTTCGGTTTTTGCATTTTCCTTATCTGCTGACCTTACAGCTGAAAAGATTCG ATTTTGATTATACAACCATGCATAGGATTAAACTGAATGATCGAATGACATTTCCCGAGGAACTAGATAT GAGTACTTTTATTGATGTTGAAGATGAGAAATCTCCTCAGACTGAAAGTTGCACTGACAGTGGAGCAGAA AATGAAGGTAGTTGTCACAGTGATCAGATGAGCAACGATTTCTCCAATGATGATGGTGTTGATGAAGGAA TCTGTCTTGAAACCAATAGTGGAACTGAAAAGATCTCAAAATCTGGACTTGAAAAGAATTCCTTGATCTA TGAACTTTTCTCTGTTATGGTTCATTCTGGGAGCGCTGCTGGTGGTCATTATTATGCATGTATAAAGTCA TTCAGTGATGAGCAGTGGTACAGCTTCAATGATCAACATGTCAGCAGGATAACACAAGAGGACATTAAGA AAACACATGGTGGATCTTCAGGAAGCAGAGGATATTATTCTAGTGCTTTCGCAAGTTCCACAAATGCATA TATGCTGATCTATAGACTGAAGGATCCAGCCAGAAATGCAAAATTTCTAGAAGTGGATGAATACCCAGAA CATATTAAAAACTTGGTGCAGAAAGAGAGAGAGTTGGAAGAACAAGAAAAGAGACAACGAGAAATTGAGC GCAATACATGCAAGATAAAATTATTCTGTTTGCATCCTACAAAACAAGTAATGATGGAAAATAAATTGGA GGTTCATAAGGATAAGACATTAAAGGAAGCAGTAGAAATGGCTTATAAGATGATGGATTTAGAAGAGGTA ATACCCCTGGATTGCTGTCGCCTTGTTAAATATGATGAGTTTCATGATTATCTAGAACGGTCATATGAAG GAGAAGAAGATACACCAATGGGGCTTCTACTAGGTGGCGTCAAGTCAACATATATGTTTGATCTGCTGTT GGAGACGAGAAAGCCTGATCAGGTTTTCCAATCTTATAAACCTGGAGAAGTGATGGTGAAAGTTCATGTT GTTGATCTAAAGGCAGAATCTGTAGCTGCTCCTATAACTGTTCGTGCTTACTTAAATCAGACAGTTACAG AATTCAAACAACTGATTTCAAAGGCCATCCATTTACCTGCTGAAACAATGAGAATAGTGCTGGAACGCTG CTACAATGATTTGCGTCTTCTCAGTGTCTCCAGTAAAACCCTGAAAGCTGAAGGATTTTTTAGAAGTAAC AAGGTGTTTGTTGAAAGCTCCGAGACTTTGGATTACCAGATGGCCTTTGCAGACTCTCATTTATGGAAAC TCCTGGATCGGCATGCAAATACAATCAGATTATTTGTTTTGCTACCTGAACAATCCCCAGTATCTTATTC CAAAAGGACAGCATACCAGAAAGCTGGAGGCGATTCTGGTAATGTGGATGATGACTGTGAAAGAGTCAAA GGACCTGTAGGAAGCCTAAAGTCTGTGGAAGCTATTCTAGAAGAAAGCACTGAAAAACTCAAAAGCTTGT CACTGCAGCAACAGCAGGATGGAGATAATGGGGACAGCAGCAAAAGTACTGAGACAAGTGACTTTGAAAA CATCGAATCACCTCTCAATGAGAGGGACTCTTCAGCATCAGTGGATAATAGAGAACTTGAACAGCATATT CAGACTTCTGATCCAGAAAATTTTCAGTCTGAAGAACGATCAGACTCAGATGTGAATAATGACAGGAGTA CAAGTTCAGTGGACAGTGATATTCTTAGCTCCAGTCATAGCAGTGATACTTTGTGCAATGCAGACAATGC TCAGATCCCTTTGGCTAATGGACTTGACTCTCACAGTATCACAAGTAGTAGAAGAACGAAAGCAAATGAA GGGAAAAAAGAAACATGGGATACAGCAGAAGAAGACTCTGGAACTGATAGTGAATATGATGAGAGTGGCA AGAGTAGGGGAGAAATGCAGTACATGTATTTCAAAGCTGAACCTTATGCTGCAGATGAAGGTTCTGGGGA AGGACATAAATGGTTGATGGTGCATGTTGATAAAAGAATTACTCTGGCAGCTTTCAAACAACATTTAGAG CCCTTTGTTGGAGTTTTGTCCTCTCACTTCAAGGTCTTTCGAGTGTATGCCAGCAATCAAGAGTTTGAGA GCGTCCGGCTGAATGAGACACTTTCATCATTTTCTGATGACAATAAGATTACAATTAGACTGGGGAGAGC ACTTAAAAAAGGAGAATACAGAGTTAAAGTATACCAGCTTTTGGTCAATGAACAAGAGCCATGCAAGTTT CTGCTAGATGCTGTGTTTGCTAAAGGAATGACTGTACGGCAATCAAAAGAGGAATTAATTCCTCAGCTCA GGGAGCAATGTGGTTTAGAGCTCAGTATTGACAGGTTTCGTCTAAGGAAAAAAACATGGAAGAATCCTGG CACTGTCTTTTTGGATTATCATATTTATGAAGAAGATATTAATATTTCCAGCAACTGGGAGGTTTTCCTT GAAGTTCTTGATGGGGTAGAGAAGATGAAGTCCATGTCACAGCTTGCAGTTTTGTCAAGACGGTGGAAGC CTTCAGAGATGAAGTTGGATCCCTTCCAGGAGGTTGTATTGGAAAGCAGTAGTGTGGACGAATTGCGAGA GAAGCTTAGTGAAATCAGTGGGATTCCTTTGGATGATATTGAATTTGCTAAGGGTAGAGGAACATTTCCC TGTGATATTTCTGTCCTTGATATTCATCAGGATTTAGACTGGAATCCTAAAGTTTCTACCCTGAATGTCT GGCCTCTTTATATCTGTGATGATGGTGCGGTCATATTTTATAGGGATAAAACAGAAGAATTAATGGAATT GACAGATGAGCAAAGAAATGAACTGATGAAAAAAGAAAGCAGTCGACTCCAGAAGACTGGACATCGTGTA ACATACTCACCTCGTAAAGAGAAAGCACTAAAAATATATCTGGATGGAGCACCAAATAAAGATCTGACTC AAGACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001282659
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282659.1, NP_001269588.1
RefSeq Size	7990 bp
RefSeq ORF	4068 bp
Locus ID	55031
UniProt ID	Q96K76
Gene Summary	Ubiquitin-specific protease that specifically deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizing POLB thereby playing a role in base-excision repair (BER). Acts as a regulator of cell growth and genome integrity. May also indirectly regulate CDC25A expression at a transcriptional level.[UniProtKB/Swiss-Prot Function]

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