EMSY (NM_001300943) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C11orf30; GL002
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300943, the custom clone sequence may differ by one or more nucleotides ATGCCTGTTGTGTGGCCAACCCTTCTGGATCTCAGCAGGGATGAATGCAAAAGAATTCTTCGAAAATTGG AATTGGAGGCATATGCTGGAGTTATCAGTGCACTTCGGGCACAGGGGGATCTCACCAAGGAAAAGAAAGA TCTTCTTGGAGAACTATCAAAAGTTCTTAGCATCTCAACAGAACGCCACCGTGCTGAAGTTCGGAGAGCA GTAAACGATGAACGGTTAACAACAATTGCACATAATATGTCTGGACCTAATAGCTCTTCAGAATGGTCCA TTGAAGGTCGTCGATTGGTACCACTGATGCCCCGGCTCGTTCCCCAAACCGCCTTTACTGTAACAGCTAA TGCTGTTGCTAATGCAGCTATCCAGCATAATGCATCTCTTCCAGTGCCTGCAGAAACAGGAAGCAAGGAA GTAGTGGTTTGCTATTCCTACACAAGTACCACGTCAACCCCAACCTCTACCCCTGTTCCAAGTGGCAGCA TAGCAACGGTTAAGTCTCCAAGACCTGCCAGTCCTGCCTCCAATGTAGTTGTCTTGCCAAGTGGAAGTAC TGTTTATGTCAAAAGTGTAAGCTGTTCAGATGAAGATGAAAAACCCAGAAAACGAAGGCGAACAAACTCT TCCAGCTCCTCTCCTGTTGTTCTAAAGGAAGTTCCAAAGGCCGTTGTTCCAGTCTCAAAGACGATCACTG TGCCTGTGAGTGGTAGTCCCAAGATGAGCAACATCATGCAGAGCATTGCCAACTCCTTACCACCCCACAT GTCTCCTGTAAAAATAACCTTCACTAAACCATCAACACAGACAACAAACACAACAACACAGAAGGTTATT ATAGTCACCACATCACCAAGCTCAACCTTCGTGCCCAACATTCTCTCCAAATCCCATAACTATGCAGCAG TCACTAAGCTTGTACCAACGTCAGTCATTGCTTCTACAACCCAGAAGCCACCAGTTGTTATAACTGCTTC ACAGTCCTCTCTGGTCAGTAATAGCAGCAGTGGCAGCAGCAGTTCTACACCATCACCTATTCCTAATACA GTTGCAGTAACAGCTGTGGTGTCCTCTACACCATCTGTGGTCATGTCAACAGTAGCACAAGGTGTATCTA CATCAGCAATCAAAATGGCATCAACCAGACTTCCTTCCCCCAAAAGCTTAGTGAGTGCCCCAACTCAGAT TCTTGCACAGTTCCCTAAACAACATCAACAGTCTCCTAAGCAGCAGTTATATCAAGTGCAACAGCAGACA CAGCAACAAGTGGCCCAGCCTTCTCCAGTATCTCATCAGCAACAGCCTCAGCAGTCTCCTTTGCCACCTG GTATTAAACCTACCATCCAAATCAAACAGGAGTCAGGTGTTAAAATCATCACACAACAGGTTCAACCAAG TAAAATCTTACCCAAACCAGTGACAGCAACTCTACCCACCAGTAGCAATTCCCCTATTATGGTGGTTAGC AGTAATGGTGCAATTATGACAACTAAACTGGTAACCACTCCTACTGGCACACAAGCAACCTATACCCGGC CAACAGTGAGCCCATCCATTGGTCGGATGGCTGCAACCCCTGGAGCTGCAACCTATGTGAAAACTACGAG TGGTAGCATCATTACAGTAGTACCCAAATCATTAGCTACCTTGGGGGGCAAGATAATTAGCAGTAATATA GTTTCTGGAACGACTACCAAAATCACTACAATCCCAATGACTTCCAAGCCCAACGTGATTGTTGTACAAA AGACTACAGGAAAAGGAACGACCATTCAAGGCCTCCCGGGCAAAAATGTTGTCACAACGTTGCTAAATGC TGGAGGAGAAAAGACTATTCAGACAGTGCCAACAGGAGCAAAGCCAGCTATCCTTACTGCTACAAGACCC ATCACCAAAATGATTGTAACGCAGCCAAAAGGAATAGGTTCTACAGTTCAACCAGCAGCTAAAATCATCC CAACAAAAATTGTTTATGGGCAGCAAGGGAAAACGCAGGTTCTTATTAAACCCAAACCAGTGACTTTTCA AGCGACAGTTGTTAGTGAACAAACAAGACAGCTAGTAACAGAAACATTACAGCAAGCATCCAGGGTAGCA GAGGCTGGTAATTCATCTATTCAGGAAGGAAAAGAAGAACCACAGAATTATACAGATAGTAGTTCCTCTT CTACAGAGTCCTCCCAGAGTTCCCAAGATTCCCAGCCTGTAGTTCATGTAATTGCTTCCCGGCGTCAGGA TTGGTCAGAACATGAGATTGCAATGGAGACTAGCCCTACCATAATTTATCAGGATGTATCCAGTGAATCA CAATCAGCTACTTCAACAATCAAAGCTCTGTTAGAACTCCAACAGACAACAGTAAAGGAAAAATTGGAAT CTAAACCAAGACAACCCACTATTGACCTGAGTCAAATGGCAGTGCCTATTCAGATGACCCAGGAAAAGAG ACATTCTCCTGAGAGTCCATCAATTGCTGTGGTAGAGTCAGAACTAGTAGCTGAATACATCACTACTGAA CGCACTGATGAGGGGACAGAGGTTGCTTTTCCCCTTCTAGTCAGCCATCGCTCCCAGCCCCAACAGCCTT CCCAGCCCCAGCGGACCCTGCTCCAGCATGTGGCTCAGTCACAGACCGCAACACAGACTTCGGTGGTGGT GAAGTCCATCCCAGCATCTTCCCCTGGAGCAATCACCCACATTATGCAGCAGGCATTAAGCAGTCACACT GCTTTTACCAAACACAGCGAGGAACTTGGAACTGAGGAGGGCGAGGTTGAAGAGATGGACACTTTAGACC CTCAGACAGGTCTGTTTTACCGATCTGCCCTGACTCAGTCACAGTCAGCTAAACAGCAGAAACTTAGCCA GCCCCCGCTGGAACAGACTCAGCTGCAAGTGAAAACTCTGCAGTGCTTCCAGACTAAACAGAAGCAGACC ATCCACCTGCAGGCAGACCAGCTCCAGCACAAACTCCCGCAAATGCCCCAGCTTTCCATCAGGCATCAAA AACTCACCCCTCTCCAGCAAGAACAAGCACAGCCCAAGCCAGATGTACAGCACACACAGCATCCCATGGT GGCCAAAGACAGGCAGCTTCCTACCTTAATGGCACAGCCCCCGCAAACTGTAGTACAGGTGCTTGCAGTG AAAACCACGCAGCAGCTCCCTAAACTGCAGCAGGCTCCGAACCAACCAAAAATCTACGTGCAACCCCAAA CCCCCCAGAGCCAAATGTCGCTCCCAGCTTCTTCAGAGAAACAGACGGCAAGCCAGGTGGAGCAGCCAAT TATAACCCAAGGATCCTCTGTTACAAAGATAACTTTTGAGGGGCGCCAGCCTCCCACAGTTACAAAGATA ACTGGTGGCAGTTCTGTGCCTAAGCTGACATCACCAGTTACAAGCATATCTCCCATTCAGGCCTCTGAGA AGACAGCAGTGTCTGACATTTTGAAAATGTCTTTGATGGAAGCTCAGATTGATACAAATGTAGAACATAT GATAGTGGATCCCCCAAAGAAGGCTCTTGCCACTAGCATGCTCACTGGTGAAGCAGGATCATTACCCTCC ACCCACATGGTGGTGGCAGGGATGGCGAATTCCACTCCCCAGCAACAGAAATGTAGAGAGTCCTGTTCGA GTCCATCCACTGTTGGCTCTTCCCTAACGACAAGGAAAATTGATCCACCAGCAGTGCCTGCGACAGGCCA GTTCATGCGTATTCAGAATGTAGGCCAAAAGAAAGCTGAAGAGAGTCCAGCAGAAATTATCATCCAGGCT ATTCCTCAGTATGCTATTCCTTGTCACTCCAGCTCCAATGTGGTGGTGGAGCCCAGTGGGCTTCTTGAGC TAAACAACTTCACTAGTCAACAGCTGGATGATGAGGAGACAGCAATGGAGCAGGACATAGACAGTAGCAC GGAGGATGGAACTGAACCCAGCCCTTCTCAGAGCTCTGCTGAACGGTCCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001300943
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300943.1, NP_001287872.1
RefSeq Size	6874 bp
RefSeq ORF	3972 bp
Locus ID	56946
UniProt ID	Q7Z589
Gene Summary	Regulator which is able to repress transcription, possibly via its interaction with a multiprotein chromatin remodeling complex that modifies the chromatin. Its interaction with BRCA2 suggests that it may play a central role in the DNA repair function of BRCA2. As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' end compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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