SIK3 (NM_001281749) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	L19; QSK; SEMDK; SIK-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001281749, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGGCGGCGGCGAGCGGAGCTGGCGGGGCTGCCGGGGCCGGGACTGGGGGAGCCGGGCCCGCGG GCCGCCTGCTGCCTCCGCCCGCGCCGGGGTCCCCAGCCGCCCCCGCTGCCGTGTCCCCTGCGGCCGGCCA GCCGCGTCCCCCAGCCCCGGCCTCCCGCGGACCCATGCCCGCCCGTATCGGCTACTACGAGATCGACCGC ACCATCGGCAAGGGCAACTTCGCGGTGGTCAAGCGGGCCACGCACCTCGTCACCAAGGCCAAGGTTGCTA TCAAGATCATAGATAAGACCCAGCTGGATGAAGAAAACTTGAAGAAGATTTTCCGGGAAGTTCAAATTAT GAAGATGCTTTGCCACCCCCATATCATCAGGCTCTACCAGGTTATGGAGACAGAACGGATGATTTATCTG GTGACAGAATATGCTAGTGGAGGGGAAATATTTGACCACCTGGTGGCCCATGGTAGAATGGCAGAAAAGG AGGCACGTCGGAAGTTCAAACAGATCGTCACAGCTGTCTATTTTTGTCACTGTCGGAACATTGTTCATCG TGATTTAAAAGCTGAAAATTTACTTCTGGATGCCAATCTGAATATCAAAATAGCAGATTTTGGTTTCAGT AACCTCTTCACTCCTGGGCAGCTGCTGAAGACCTGGTGTGGCAGCCCTCCCTATGCTGCACCTGAACTCT TTGAAGGAAAAGAATATGATGGGCCCAAAGTGGACATCTGGAGCCTTGGAGTTGTCCTCTACGTGCTTGT GTGCGGTGCCCTGCCATTTGATGGAAGCACACTGCAGAATCTGCGGGCCCGCGTGCTGAGTGGAAAGTTC CGCATCCCATTTTTTATGTCCACAGAATGTGAGCATTTGATCCGCCATATGTTGGTGTTAGATCCCAATA AGCGCCTCTCCATGGAGCAGATCTGCAAGCACAAGTGGATGAAGCTAGGGGACGCCGATCCCAACTTTGA CAGGTTAATAGCTGAATGCCAACAACTAAAGGAAGAAAGACAGGTGGACCCCCTGAATGAGGATGTCCTC TTGGCCATGGAGGACATGGGACTGGACAAAGAACAGACACTGCAGGCGGAGCAGGCAGGTACTGCTATGA ACATCAGCGTTCCCCAGGTGCAGCTGATCAACCCAGAGAACCAAATTGTGGAGCCGGATGGGACACTGAA TTTGGACAGTGATGAGGGTGAAGAGCCTTCCCCTGAAGCATTGGTGCGCTATTTGTCAATGAGGAGGCAC ACAGTGGGTGTGGCTGACCCACGCACGGAAGTTATGGAAGATCTGCAGAAGCTCCTACCTGGCTTTCCTG GAGTCAACCCCCAGGCTCCATTCCTGCAGGTGGCCCCTAATGTGAACTTCATGCACAACCTGTTGCCTAT GCAAAACTTGCAACCAACCGGGCAACTTGAGTACAAGGAGCAGTCTCTCCTACAGCCGCCCACGCTACAG CTGTTGAATGGAATGGGCCCCCTTGGCCGGAGGGCATCAGATGGAGGAGCCAACATCCAACTGCATGCCC AGCAGCTGCTGAAGCGCCCACGGGGACCCTCTCCGCTTGTCACCATGACACCAGCAGTGCCAGCAGTTAC CCCTGTGGACGAGGAGAGCTCAGACGGGGAGCCAGACCAGGAAGCTGTGCAGAGGTACTTGGCAAATAGG TCCAAAAGACATACACTGGCCATGACCAACCCTACAGCTGAGATCCCACCGGACCTACAACGGCAGCTAG GACAGCAGCCTTTCCGTTCCCGGGTCTGGCCTCCTCACCTGGTACCTGATCAGCATCGCTCTACCTACAA GGACTCCAACACTCTGCACCTCCCTACGGAGCGTTTCTCCCCTGTGCGCCGGTTCTCAGATGGGGCTGCG AGCATCCAGGCCTTCAAAGCTCACCTGGAAAAAATGGGCAACAACAGCAGCATCAAACAGCTGCAGCAGG AGTGTGAGCAGCTGCAGAAGATGTACGGGGGGCAGATTGATGAAAGAACCCTGGAGAAGACCCAGCAGCA GCATATGTTATACCAGCAGGAGCAGCACCATCAAATTCTCCAGCAACAAATTCAAGACTCTATCTGTCCT CCTCAGCCATCTCCACCTCTTCAGGCTGCATGTGAAAATCAGCCAGCCCTCCTTACCCATCAGCTCCAGA GGTTAAGGATTCAGCCTTCAAGCCCACCCCCCAACCACCCCAACAACCATCTCTTCAGGCAGCCCAGTAA TAGTCCTCCCCCCATGAGCAGTGCCATGATCCAGCCTCACGGGGCTGCATCTTCTTCCCAGTTTCAAGGC TTACCTTCCCGCAGTGCAATCTTTCAGCAGCAACCTGAGAACTGTTCCTCTCCTCCCAACGTGGCACTAA CCTGCTTGGGTATGCAGCAGCCTGCTCAGTCACAGCAGGTCACCATCCAAGTCCAAGAGCCTGTTGACAT GCTCAGCAACATGCCAGGCACAGCTGCAGGCTCCAGTGGGCGCGGCATCTCCATCAGCCCCAGTGCTGGT CAGATGCAGATGCAGCACCGTACCAACCTGATGGCCACCCTCAGCTATGGGCACCGTCCCTTGTCCAAGC AGCTGAGTGCTGACAGTGCAGAGGCTCACAGTGCACATCAGCAGCCGCCACACTATACCACGTCGGCACT ACAGCAGGCCCTGCTGTCTCCCACGCCGCCAGACTATACAAGACACCAGCAGGTACCCCACATCCTTCAA GGACTGCTTTCTCCCCGGCATTCGCTCACCGGCCACTCGGACATCCGGCTGCCCCCAACAGAGTTTGCAC AGCTCATTAAAAGGCAGCAGCAACAACGGCAGCAGCAGCAGCAACAGCAGCAACAGCAAGAATACCAGGA ACTGTTCAGGCACATGAACCAAGGGGATGCGGGGAGTCTGGCTCCCAGCCTTGGGGGACAGAGCATGACA GAGCGCCAGGCTTTATCTTATCAAAATGCTGACTCTTATCACCATCACACCAGCCCCCAGCATCTGCTAC AAATCAGGGCACAAGAATGTGTCTCACAGGCTTCCTCACCCACCCCGCCCCACGGGTATGCTCACCAGCC GGCACTGATGCATTCAGAGAGCATGGAGGAGGACTGCTCGTGTGAGGGGGCCAAGGATGGCTTCCAAGAC AGTAAGAGTTCAAGTACATTGACCAAAGGTTGCCATGACAGCCCTCTGCTCTTGAGTACCGGTGGACCTG GGGACCCTGAATCTTTGCTAGGAACTGTGAGTCATGCCCAAGAATTGGGGATACATCCCTATGGTCATCA GCCAACTGCTGCATTCAGTAAAAATAAGGTGCCCAGCAGAGAGCCTGTCATAGGGAACTGCATGGATAGA AGTTCTCCAGGACAAGCAGTGGAGCTGCCGGATCACAATGGGCTCGGGTACCCAGCACGCCCCTCCGTCC ATGAGCACCACAGGCCCCGGGCCCTCCAGAGACACCACACGATCCAGAACAGCGACGATGCTTATGTACA GCTGGATAACTTGCCAGGAATGAGTCTCGTGGCTGGGAAAGCACTTAGCTCTGCCCGGATGTCGGATGCA GTTCTCAGTCAGTCTTCGCTCATGGGCAGCCAGCAGTTTCAGGATGGGGAAAATGAGGAATGTGGGGCAA GCCTGGGAGGTCATGAGCACCCAGACCTGAGTGATGGCAGCCAGCATTTAAACTCCTCTTGCTATCCATC TACGTGTATTACAGACATTCTGCTCAGCTACAAGCACCCCGAAGTCTCCTTCAGCATGGAGCAGGCAGGC GTGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001281749
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281749.1, NP_001268678.1
RefSeq Size	6039 bp
RefSeq ORF	3786 bp
Locus ID	23387
UniProt ID	Q9Y2K2
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	Positive regulator of mTOR signaling that functions by triggering the degradation of DEPTOR, an mTOR inhibitor. Involved in the dynamic regulation of mTOR signaling in chondrocyte differentiation during skeletogenesis (PubMed:30232230). Negatively regulates cAMP signaling pathway possibly by acting on CRTC2/TORC2 and CRTC3/TORC3 (Probable). Prevents HDAC4 translocation to the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This results in a shorter isoform (3) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.