RPB2 (POLR2B) (NM_001303268) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	hRPB140; POL2RB; RPB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001303268, the custom clone sequence may differ by one or more nucleotides ATGCTGGATTGTAATCAGCCACGATATTTGCTGAAGTTTGAACAAATTTATCTTTCCAAGCCTACCCATT GGGAAAGAGATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAATCTCACGTATTCTGC TCCGCTTTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTTCAGACTCAGCATCAG AAAACTTTTATAGGAAAAATTCCAATTATGTTGCGGTCAACTTACTGCCTTTTGAATGGCTTGACAGATC GTGATCTTTGTGAGTTAAATGAATGCCCTTTGGATCCTGGTGGCTATTTCATTATTAATGGATCAGAAAA GGTTCTGATTGCCCAAGAGAAAATGGCAACAAACACAGTTTATGTGTTTGCCAAAAAGGATTCTAAATAT GCCTACACAGGAGAGTGTAGATCATGTCTTGAGAATTCTTCCCGACCCACCAGTACTATATGGGTTAGCA TGCTGGCAAGAGGAGGACAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTACCATA TATCAAGCAAGAAGTTCCCATCATTATTGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGATATTTTA GAACATATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGTTAAACCTTCTCTCGATGAAGCTT TTGTCATCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGTGTTACTAA AGAGAAAAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGTGTCAGTGAT TTTTGTGAGACCAAAAAAGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCAGCTTTGGGTA GAAGAGAACTAGATGACAGAGATCACTATGGAAACAAGAGATTGGATCTTGCTGGGCCGCTGCTTGCATT CTTATTTAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAAATTTATTGATCGA GGAAAGGATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTAAAATACTCTTTAG CTACTGGAAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCTCAGGTGTTAAACCG CCTGACTTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAATTCTCCTATTGGTAGAGACGGCAAGCTA GCAAAACCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCCTGCCGAGACCCCAGAGGGCCATG CTGTAGGACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCAACCATCTCCAATTCT GGAATTTTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACC AAGATTTTTGTTAATGGCTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTATGAACACCCTAAGGA AATTGAGACGTCAGATGGACATCATTGTGTCTGAAGTTTCTATGATCAGAGATATTCGAGAGAGGGAGAT TCGGATCTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAACAAAAGCTACTTTTG AAGAAGAGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGCAGGATCTTGTGGCCA GTGGGGTAGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAATGACTCCAGATGATTT ACAGGAGAAAGAAGTAGCTTATTGTTCCACATATACACACTGTGAGATTCATCCCTCAATGATCCTTGGT GTCTGTGCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAGAAACACATACCAGTCTGCTATGG GTAAGCAGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATTGGCCCATGTTCTCTA TTATCCTCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGAGAGCTGCCAGCAGGC ATCAACTCAATTGTGGCCATTGCATCATACACTGGATATAATCAGGAAGACTCTGTTATCATGAATCGTT CAGCTGTAGACCGCGGCTTCTTCAGGTCTGTTTTCTATCGCTCATACAAAGAACAGGAGTCTAAAAAAGG ATTTGATCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATGAGGCATGCCATTTAC GACAAGCTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATGATGTTATTATAGGCA AAACAGTCACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTATACCAAGAGAGACTG TAGCACTTTTCTCAGAACTAGCGAGACGGGCATTGTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGA TATAAATTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGATTGGAGACAAATTTGCTAGTCGAC ATGGTCAAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCACCTGTGAAGGTATCAC CCCTGATATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTT CAAGGGAAGGTATCGGCTAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGC AGAAGATTTCTAATCTTTTATCTGATTATGGCTATCATCTCAGAGGAAATGAGGTCCTGTACAATGGGTT CACTGGTCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGTTTGAAGCATATGGTG GATGATAAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGAT CTCGTGATGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTT TTTAAGGGAAAGATTGTTTGAGGCATCAGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATG GCGATTGCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCGCAATAAAACCCAGATTTCTTTGG TGCGAATGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAATGAT GAGTGTTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001303268
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001303268.1, NP_001290197.1
RefSeq Size	3911 bp
RefSeq ORF	3300 bp
Locus ID	5431
UniProt ID	P30876
Protein Families	Transcription Factors
Protein Pathways	Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
Gene Summary	This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014] Transcript Variant: This variant (2) differs in the 5' UTR, lacks an alternate exon in the 5' coding region, and initiates translation at an alternate downstream start codon, compared to variant 1. This results in isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.