Anillin (ANLN) (NM_001284302) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FSGS8; scra; Scraps
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284302, the custom clone sequence may differ by one or more nucleotides ATGGATCCGTTTACGGAGAAACTGCTGGAGCGAACCCGTGCCAGGCGAGAGAATCTTCAGAGAAAAATGG CTGAGAGGCCCACAGCAGCTCCAAGGTCTATGACTCATGCTAAGCGAGCTAGACAGCCACTTTCAGAAGC AAGTAACCAGCAGCCCCTCTCTGGTGGTGAAGAGAAATCTTGTACAAAACCATCGCCATCAAAAAAACGC TGTTCTGACAACACTGAAGTAGAAGTTTCTAACTTGGAAAATAAACAACCAGTTGAGTCGACATCTGCAA AATCTTGTTCTCCAAGTCCTGTGTCTCCTCAGGTGCAGCCACAAGCAGCAGATACCATCAGTGATTCTGT TGCTGTCCCGGCATCACTGCTGGGCATGAGGAGAGGGCTGAACTCAAGATTGGAAGCAACTGCAGCCTCC TCAGTTAAAACACGTATGCAAAAACTTGCAGAGCAACGGCGCCGTTGGGATAATGATGATATGACAGATG ACATTCCTGAAAGCTCACTCTTCTCACCAATGCCATCAGAGGAAAAGGCTGCTTCCCCTCCCAGACCTCT GCTTTCAAATGCCTCGGCAACTCCAGTTGGCAGAAGGGGCCGTCTGGCCAATCTTGCTGCAACTATTTGC TCCTGGGAAGATGATGTAAATCACTCATTTGCAAAACAAAACAGTGTACAAGAACAGCCTGGTACCGCTT GTTTATCCAAATTTTCCTCTGCAAGTGGAGCATCTGCTAGGATCAATAGCAGCAGTGTTAAGCAGGAAGC TACATTCTGTTCCCAAAGGGATGGCGATGCCTCTTTGAATAAAGCCCTATCCTCAAGTGCTGATGATGCG TCTTTGGTTAATGCCTCAATTTCCAGCTCTGTGAAAGCTACTTCTCCAGTGAAATCTACTACATCTATCA CTGATGCTAAAAGTTGTGAGGGACAAAATCCTGAGCTACTTCCAAAAACTCCTATTAGTCCTCTGAAAAC GGGGGTATCGAAACCAATTGTGAAGTCAACTTTATCCCAGACAGTTCCATCCAAGGGAGAATTAAGTAGA GAAATTTGTCTGCAATCTCAATCTAAAGACAAATCTACGACACCAGGAGGAACAGGAATTAAGCCTTTCC TGGAACGCTTTGGAGAGCGTTGTCAAGAACATAGCAAAGAAAGTCCAGCTCGTAGCACACCCCACAGAAC CCCCATTATTACTCCAAATACAAAGGCCATCCAAGAAAGATTATTCAAGCAAGACACATCTTCATCTACT ACCCATTTAGCACAACAGCTCAAGCAGGAACGTCAAAAAGAACTAGCATGTCTTCGTGGCCGATTTGACA AGGGCAATATATGGAGTGCAGAAAAAGGCGGAAACTCAAAAAGCAAACAACTAGAAACCAAACAGGAAAC TCACTGTCAGAGCACTCCCCTCAAAAAACACCAAGGTGTTTCAAAAACTCAGTCACTTCCAGTAACAGAA AAGGTGACCGAAAACCAGATACCAGCCAAAAATTCTAGTACAGAACCTAAAGTGATACGTGAAATTGAGA TGAGTGTGGATGATGATGATATCAATAGTTCGAAAGTAATTAATGACCTCTTCAGTGATGTCCTAGAGGA AGGTGAACTAGATATGGAGAAGAGCCAAGAGGAGATGGATCAAGCATTAGCAGAAAGCAGCGAAGAACAG GAAGATGCACTGAATATCTCCTCAATGTCTTTACTTGCACCATTGGCACAAACAGTTGGTGTGGTAAGTC CAGAGAGTTTAGTGTCCACACCTAGACTGGAATTGAAAGACACCAGCAGAAGTGATGAAAGTCCAAAACC AGGAAAATTCCAAAGAACTCGTGTCCCTCGAGCTGAATCTGGTGATAGCCTTGGTTCTGAAGATCGTGAT CTTCTTTACAGCATTGATGCATATAGATCTCAAAGATTCAAAGAAACAGAACGTCCATCAATAAAGCAGG TGATTGTTCGGAAGGAAGATGTTACTTCAAAACTGGATGAAAAAAATAATGCCTTTCCTTGTCAAGTTAA TATCAAACAGAAAATGCAGGAACTCAATAACGAAATAAATATGCAACAGACAGTGATCTATCAAGCTAGC CAGGCTCTTAACTGCTGTGTTGATGAAGAACATGGAAAAGGGTCCCTAGAAGAAGCTGAAGCAGAAAGAC TTCTTCTAATTGCAACTGGGAAGAGAACACTTTTGATTGATGAATTGAATAAATTGAAGAACGAAGGACC TCAGAGGAAGAATAAGGCTAGTCCCCAAAGTGAATTTATGCCATCCAAAGGATCAGTTACTTTGTCAGAA ATCCGCTTGCCTCTAAAAGCAGATTTTGTCTGCAGTACGGTTCAGAAACCAGATGCAGCAAATTACTATT ACTTAATTATACTAAAAGCAGGAGCTGAAAATATGGTAGCCACACCATTAGCAAGTACTTCAAACTCTCT TAACGGTGATGCTCTGACATTCACTACTACATTTACTCTGCAAGATGTATCCAATGACTTTGAAATAAAT ATTGAAGTTTACAGCTTGGTGCAAAAGAAAGATCCCTCAGGCCTTGATAAGAAGAAAAAAACATCCAAGT CCAAGGCTATTACTCCAAAGCGACTCCTCACATCTATAACCACAAAAAGCAACATTCATTCTTCAGTCAT GGCCAGTCCAGGAGGTCTTAGTGCTGTGCGAACCAGCAACTTCGCCCTTGTTGGATCTTACACATTATCA TTGTCTTCAGTAGGAAATACTAAGTTTGTTCTGGACAAGGTCCCCTTTTTATCTTCTTTGGAAGGTCATA TTTATTTAAAAATAAAATGTCAAGTGAATTCCAGTGTTGAAGAAAGAGGTTTTCTAACCATATTTGAAGA TGTTAGTGGTTTTGGTGCCTGGCATCGAAGATGGTGTGTTCTTTCTGGAAACTGTATATCTTATTGGACT TATCCAGATGATGAGAAACGCAAGAATCCCATAGGAAGGATAAATCTGGCTAATTGTACCAGTCGTCAGA TAGAACCAGCCAACAGAGAATTTTGTGCAAGACGCAACACTTTTGAATTAATTACTGTCCGACCACAAAG AGAAGATGACCGAGAGACTCTTGTCAGCCAATGCAGGGACACACTCTGTGTTACCAAGAACTGGCTGTCT GCAGATACTAAAGAAGAGCGGGATCTCTGGATGCAAAAACTCAATCAAGTTCTTGTTGATATTCGCCTCT GGCAACCTGATGCTTGCTACAAACCTATTGGAAAGCCTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001284302
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284302.2, NP_001271231.1
RefSeq Size	4690 bp
RefSeq ORF	3261 bp
Locus ID	54443
UniProt ID	Q9NQW6
Protein Families	Druggable Genome
Gene Summary	This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (3) lacks an in-frame exon and uses an alternate in-frame splice site compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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