USP7 (NM_001286457) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HAFOUS; HAUSP; TEF1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286457, the custom clone sequence may differ by one or more nucleotides ATGGCTGGGAACCACAGGCTTGGGCTCGAAGCGGGAGATACAGATGACCCACCAAGAATTACTCAGAACC CTGTGATCAATGGGAATGTGGCCCTGAGTGATGGACACAACACCGCGGAGGAGGACATGGAGGATGACAC CAGTTGGCGCTCCGAGGCAACCTTTCAGTTCACTGTGGAGCGCTTCAGCAGACTGAGTGAGTCGGTCCTT AGCCCTCCGTGTTTTGTGCGAAATCTGCCATGGAAGATTATGGTGATGCCACGCTTTTATCCAGACAGAC CACACCAAAAAAGCGTAGGATTCTTTCTCCAGTGCAATGCTGAATCTGATTCCACGTCATGGTCTTGCCA TGCACAAGCAGTGCTGAAGATAATAAATTACAGAGATGATGAAAAGTCGTTCAGTCGTCGTATTAGTCAT TTGTTCTTCCATAAAGAAAATGATTGGGGATTTTCCAATTTTATGGCCTGGAGTGAAGTGACCGATCCTG AGAAAGGATTTATAGATGATGACAAAGTTACCTTTGAAGTCTTTGTACAGGCGGATGCTCCCCATGGAGT TGCGTGGGATTCAAAGAAGCACACAGGCTACGTCGGCTTAAAGAATCAGGGAGCGACTTGTTACATGAAC AGCCTGCTACAGACGTTATTTTTCACGAATCAGCTACGAAAGGCTGTGTACATGATGCCAACCGAGGGGG ATGATTCGTCTAAAAGCGTCCCTTTAGCATTACAAAGAGTGTTCTATGAATTACAGCATAGTGATAAACC TGTAGGAACAAAAAAGTTAACAAAGTCATTTGGGTGGGAAACTTTAGATAGCTTCATGCAACATGATGTT CAGGAGCTTTGTCGAGTGTTGCTCGATAATGTGGAAAATAAGATGAAAGGCACCTGTGTAGAGGGCACCA TACCCAAATTATTCCGCGGCAAAATGGTGTCCTATATCCAGTGTAAAGAAGTAGACTATCGGTCTGATAG AAGAGAAGATTATTATGATATCCAGCTAAGTATCAAAGGAAAGAAAAATATATTTGAATCATTTGTGGAT TATGTGGCAGTAGGACAGCTCGATGGGGACAATAAATACGACGCTGGGGAACATGGCTTACAGGAAGCAG AGAAAGGTGTGAAATTCCTAACATTGCCACCAGTGTTACATCTACAACTGATGAGATTTATGTATGACCC TCAGACGGACCAAAATATCAAGATCAATGATAGGTTTGAATTCCCAGAGCAGTTACCACTTGATGAATTT TTGCAAAAAACAGATCCTAAGGACCCTGCAAATTATATTCTTCATGCAGTCCTGGTTCATAGTGGAGATA ATCATGGTGGACATTATGTGGTTTATCTAAACCCCAAAGGGGATGGCAAATGGTGTAAATTTGATGACGA CGTGGTGTCAAGGTGTACTAAAGAGGAAGCAATTGAGCACAATTATGGGGGTCACGATGACGACCTGTCT GTTCGACACTGCACTAATGCTTACATGTTAGTCTACATCAGGGAATCAAAACTGAGTGAAGTTTTACAGG CGGTCACCGACCATGATATTCCTCAGCAGTTGGTGGAGCGATTACAAGAAGAGAAAAGGATCGAGGCTCA GAAGCGGAAGGAGCGGCAGGAAGCCCATCTCTATATGCAAGTGCAGATAGTCGCAGAGGACCAGTTTTGT GGCCACCAAGGGAATGACATGTACGATGAAGAAAAAGTGAAATACACTGTGTTCAAAGTATTGAAGAACT CCTCGCTTGCTGAGTTTGTTCAGAGCCTCTCTCAGACCATGGGATTTCCACAAGATCAAATTCGATTGTG GCCCATGCAAGCAAGGAGTAATGGAACAAAACGACCAGCAATGTTAGATAATGAAGCCGACGGCAATAAA ACAATGATTGAGCTCAGTGATAATGAAAACCCTTGGACAATATTCCTGGAAACAGTTGATCCCGAGCTGG CTGCTAGTGGAGCGACCTTACCCAAGTTTGATAAAGATCATGATGTAATGTTATTTTTGAAGATGTATGA TCCCAAAACGCGGAGCTTGAATTACTGTGGGCATATCTACACACCAATATCCTGTAAAATACGTGACTTG CTCCCAGTTATGTGTGACAGAGCAGGATTTATTCAAGATACTAGCCTTATCCTCTATGAGGAAGTTAAAC CGAATTTAACAGAGAGAATTCAGGACTATGACGTGTCTCTTGATAAAGCCCTTGATGAACTAATGGATGG TGACATCATAGTATTTCAGAAGGATGACCCTGAAAATGATAACAGTGAATTACCCACCGCAAAGGAGTAT TTCCGAGATCTCTACCACCGCGTTGATGTCATTTTCTGTGATAAAACAATCCCTAATGATCCTGGATTTG TGGTTACGTTATCAAATAGAATGAATTATTTTCAGGTTGCAAAGACAGTTGCACAGAGGCTCAACACAGA TCCAATGTTGCTGCAGTTTTTCAAGTCTCAAGGTTATAGGGATGGCCCAGGTAATCCTCTTAGACATAAT TATGAAGGTACTTTAAGAGATCTTCTACAGTTCTTCAAGCCTAGACAACCTAAGAAACTTTACTATCAGC AGCTTAAGATGAAAATCACAGACTTTGAGAACAGGCGAAGTTTTAAATGTATATGGTTAAACAGCCAATT TAGGGAAGAGGAAATAACACTATATCCAGACAAGCATGGGTGTGTCCGGGACCTGTTAGAAGAATGTAAA AAGGCCGTGGAGCTTGGGGAGAAAGCATCAGGGAAACTTAGGCTGCTAGAAATTGTAAGCTACAAAATCA TTGGTGTTCATCAAGAAGATGAACTATTAGAATGTTTATCTCCTGCAACGAGCCGGACGTTTCGAATAGA GGAAATCCCTTTGGACCAGGTGGACATAGACAAAGAGAATGAGATGCTTGTCACAGTGGCGCATTTCCAC AAAGAGGTCTTCGGAACGTTCGGAATCCCGTTTTTGCTGAGGATACACCAGGGCGAGCATTTTCGAGAAG TGATGAAGCGAATCCAGAGCCTGCTGGACATCCAGGAGAAGGAGTTTGAGAAGTTTAAATTTGCAATTGT AATGATGGGCCGACACCAGTACATAAATGAAGACGAGTATGAAGTAAATTTGAAAGACTTTGAGCCACAG CCCGGTAATATGTCTCATCCTCGGCCTTGGCTAGGGCTCGACCACTTCAACAAAGCCCCAAAGAGGAGTC GCTACACTTACCTTGAAAAGGCCATTAAAATCCATAACTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286457
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286457.1, NP_001273386.1
RefSeq Size	5281 bp
RefSeq ORF	3261 bp
Locus ID	7874
UniProt ID	Q93009
Protein Families	Druggable Genome, Protease
Gene Summary	The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder. [provided by RefSeq, Mar 2016] Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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