BUB1 (NM_001278617) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BUB1A; BUB1L; hBUB1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278617, the custom clone sequence may differ by one or more nucleotides ATGGACACCCCGGAAAATGTCCTTCAGATGCTTGAAGCCCACATGCAGAGCTACAAGGGCAATGACCCTC TTGGTGAATGGGAAAGATACATACAGTGGGTAGAAGAGAATTTTCCTGAGAATAAAGAATACTTGATAAC TTTACTAGAACATTTAATGAAGGAATTTTTAGATAAGAAGAAATACCACAATGACCCAAGATTCATCAGT TATTGTTTAAAATTTGCTGAGTACAACAGTGACCTCCATCAATTTTTTGAGTTTCTGTACAACCATGGGA TTGGAACCCTGTCATCCCCTCTGTACATTGCCTGGGCGGGGCATCTGGAAGCCCAAGGAGAGCTGCAGCA TGCCAGTGCTGTCCTTCAGAGAGGAATTCAAAACCAGGCTGAACCCAGAGAGTTCCTGCAACAACAATAC AGGTTATTTCAGACACGCCTCACTGAAACCCATTTGCCAGCTCAAGCTAGAACCTCAGAACCTCTGCATA ATGTTCAGGTTTTAAATCAAATGATAACATCAAAATCAAATCCAGGAAATAACATGGCCTGCATTTCTAA GAATCAGGGTTCAGAGCTTTCTGGAGTGATATCTTCAGCTTGTGATAAAGAGTCAAATATGGAACGAAGA GTGATCACGATTTCTAAATCAGAATATTCTGTGCACTCATCTTTGGCATCCAAAGTTGATGTTGAGCAGG TTGTTATGTATTGCAAGGAGAAGCTTATTCGTGGGGAATCAGAATTTTCCTTTGAAGAATTGAGAGCCCA GAAATACAATCAACGGAGAAAGCATGAGCAATGGGTAAATGAAGACAGACATTATATGAAAAGGAAAGAA GCAAATGCTTTTGAAGAACAGCTATTAAAACAGAAAATGGATGAACTTCATAAGAAGTTGCATCAGGTGG TGGAGACATCCCATGAGGATCTGCCCGCTTCCCAGGAAAGGTCCGAGGTTAATCCAGCACGTATGGGGCC AAGTGTAGGCTCCCAGCAGGAACTGAGAGCGCCATGTCTTCCAGTAACCTATCAGCAGACACCAGTGAAC ATGGAAAAGAACCCAAGAGAGGCACCTCCTGTTGTTCCTCCTTTGGCAAATGCTATTTCTGCAGCTTTGG TGTCCCCAGCCACCAGCCAGAGCATTGCTCCTCCTGTTCCTTTGAAAGCCCAGACAGTAACAGACTCCAT GTTTGCAGTGGCCAGCAAAGATGCTGGATGTGTGAATAAGAGTACTCATGAATTCAAGCCACAGAGTGGA GCAGAGATCAAAGAAGGGTGTGAAACACATAAGGTTGCCAACACAAGTTCTTTTCACACAACTCCAAACA CATCACTGGGAATGGTTCAGGCAACGCCATCCAAAGTGCAGCCATCACCCACCGTGCACACAAAAGAAGC ATTAGGTTTCATCATGAATATGTTTCAGGCTCCTACACTTCCTGATATTTCTGATGACAAAGATGAATGG CAATCTCTAGATCAAAATGAAGATGCATTTGAAGCCCAGTTTCAAAAAAATGTAAGGTCATCTGGGGCTT GGGGAGTCAATAAGATCATCTCTTCTTTGTCATCTGCTTTTCATGTGTTTGAAGATGGAAACAAAGAAAA TTATGGATTACCACAGCCTAAAAATAAACCCACAGGAGCCAGGACCTTTGGAGAACGCTCTGTCAGCAGA CTTCCTTCAAAACCAAAGGAGGAAGTGCCTCATGCTGAAGAGTTTTTGGATGACTCAACTGTATGGGGTA TTCGCTGCAACAAAACCCTGGCACCCAGTCCTAAGAGCCCAGGAGACTTCACATCTGCTGCACAACTTGC GTCTACACCATTCCACAAGCTTCCAGTGGAGTCAGTGCACATTTTAGAAGATAAAGAAAATGTGGTAGCA AAACAGTGTACCCAGGCGACTTTGGATTCTTGTGAGGAAAACATGGTGGTGCCTTCAAGGGATGGAAAAT TCAGTCCAATTCAAGAGAAAAGCCCAAAACAGGCCTTGTCGTCTCACATGTATTCAGCATCCTTACTTCG TCTGAGCCAGCCTGCTGCAGGTGGGGTACTTACCTGTGAGGCAGAGTTGGGCGTTGAGGCTTGCAGACTC ACAGACACTGACGCTGCCATTGCAGAAGATCCACCAGATGCTATTGCTGGGCTCCAAGCAGAATGGATGC AGATGAGTTCACTTGGGACTGTTGATGCTCCAAACTTCATTGTTGGGAACCCATGGGATGATAAGCTGAT TTTCAAACTTTTATCTGGGCTTTCTAAACCAGTGAGTTCCTATCCAAATACTTTTGAATGGCAATGTAAA CTTCCAGCCATCAAGCCCAAGACTGAATTTCAATTGGGTTCTAAGCTGGTCTATGTCCATCACCTTCTTG GAGAAGGAGCCTTTGCCCAGGTGTACGAAGCTACCCAGGGAGATCTGAATGATGCTAAAAATAAACAGAA ATTTGTTTTAAAGGTCCAAAAGCCTGCCAACCCCTGGGAATTCTACATTGGGACCCAGTTGATGGAAAGA CTAAAGCCATCTATGCAGCACATGTTTATGAAGTTCTATTCTGCCCACTTATTCCAGAATGGCAGTGTAT TAGTAGGAGAGCTCTACAGCTATGGAACATTATTAGATGATGAAGATGATTTATCTGCTGGCTTGGCACT GATTGACCTGGGTCAGAGTATAGATATGAAACTTTTTCCAAAAGGAACTATATTCACAGCAAAGTGTGAA ACATCTGGTTTTCAGTGTGTTGAGATGCTCAGCAACAAACCATGGAACTACCAGATCGATTACTTTGGGG TTGCTGCAACAGTATATTGCATGCTCTTTGGCACTTACATGAAAGTGAAAAATGAAGGAGGAGAGTGTAA GCCTGAAGGTCTTTTTAGAAGGCTTCCTCATTTGGATATGTGGAATGAATTTTTTCATGTTATGTTGAAT ATTCCAGATTGTCATCATCTTCCATCTTTGGATTTGTTAAGGCAAAAGCTGAAGAAAGTATTTCAACAAC ACTATACTAACAAGATTAGGGCCCTACGTAATAGGCTAATTGTACTGCTCTTAGAATGTAAGCGTTCACG AAAATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001278617
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278617.1, NP_001265546.1
RefSeq Size	3465 bp
RefSeq ORF	3087 bp
Locus ID	699
UniProt ID	O43683
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
Gene Summary	This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 3 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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