HA1 (ARHGAP45) (NM_001282335) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HA-1; HLA-HA1; HMHA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282335, the custom clone sequence may differ by one or more nucleotides ATGTGTATCTGCGGGACGGCGCACCCGGTGCTGGACGAGGGCCCCGTGCGCTGCCGGGCGGGCCCCCGAG ACCTCCTTGAGGCCCGCCGCCCGCGGGCCCACGAGTGCCTGGGTGAGGCTCTGCGTGTCATGCATCAGAT CATCTCCAAGTACCCGCTGCTGAACACCGTGGAGACGCTCACCGCAGCCGGCACCCTCATTGCCAAGGTC AAAGCCTTCCATTATGAGAGCAACAATGATCTGGAGAAACAGGAGTTCGAGAAGGCCCTGGAGACGATTG CTGTGGCCTTCAGTAGCACAGTGTCCGAGTTCCTCATGGGTGAAGTGGACAGCAGCACCCTCCTAGCAGT GCCTCCTGGGGACTCGAGCCAGTCCATGGAAAGCCTGTATGGACCGGGCAGTGAGGGCACGCCTCCCAGC CTGGAAGACTGTGACGCCGGCTGCCTGCCCGCCGAGGAGGTGGACGTGCTGCTACAGCGCTGTGAGGGGG GCGTGGATGCCGCACTGCTGTATGCCAAGAACATGGCCAAGTACATGAAGGACCTCATCAGCTACCTGGA GAAGCGGACGACGCTGGAGATGGAGTTTGCCAAGGGCCTGCAGAAGATCGCTCACAACTGCAGACAGAGC GTCATGCAGGAGCCCCACATGCCGCTCCTGTCCATCTACTCGCTGGCCCTGGAGCAGGACCTGGAGTTCG GCCACAGCATGGTGCAGGCGGTGGGCACCTTGCAGACCCAGACCTTCATGCAGCCCCTGACCCTGCGGCG GCTTGAACACGAGAAGCGCAGGAAGGAGATCAAGGAGGCCTGGCACCGTGCCCAGAGGAAGCTGCAAGAG GCGGAGTCCAACCTGCGCAAGGCCAAGCAGGGTTACGTGCAGCGCTGCGAGGACCACGACAAGGCTCGCT TCCTCGTGGCCAAGGCGGAGGAGGAGCAGGCTGGCAGCGCGCCGGGAGCAGGCAGCACGGCCACCAAGAC CCTGGACAAGCGGCGGCGGCTGGAGGAGGAGGCCAAGAACAAGGCGGAGGAAGCTATGGCCACCTACCGC ACCTGCGTGGCCGACGCGAAGACGCAGAAGCAGGAGCTGGAGGATACCAAGGTGACGGCGCTGCGGCAGA TCCAGGAGGTCATCCGGCAGAGCGACCAAACCATCAAGTCGGCCACGATCTCCTACTACCAGATGATGCA TATGCAGACGGCGCCGCTGCCCGTGCACTTCCAGATGCTGTGTGAGAGCAGCAAGCTGTATGACCCAGGC CAGCAGTACGCCTCCCACGTGCGCCAGCTGCAGCGGGACCAGGAGCCCGATGTGCACTACGACTTTGAGC CCCACGTCTCCGCCAACGCCTGGTCCCCCGTCATGCGTGCCCGGAAGAGCAGCTTCAACGTGAGTGATGT GGCGCGGCCGGAGGCTGCCGGGAGCCCCCCAGAAGAAGGCGGGTGCACTGAGGGCACACCTGCCAAGGAC CACAGGGCCGGGCGAGGACACCAGGTTCACAAGTCATGGCCGCTCTCGATCTCAGACTCGGACAGTGGGC TGGACCCCGGCCCTGGCGCAGGGGACTTTAAGAAGTTCGAGCGGACGTCATCCAGTGGTACCATGTCGTC CACGGAGGAGCTGGTGGACCCAGACGGTGGAGCCGGGGCTTCAGCCTTTGAGCAGGCTGACCTCAACGGC ATGACCCCCGAGCTGCCGGTGGCCGTGCCCAGTGGACCGTTCCGCCACGAGGGGCTGTCCAAGGCGGCCC GTACTCACCGGCTCCGGAAGCTCCGCACGCCCGCCAAGTGCCGCGAGTGCAACAGCTACGTCTACTTCCA GGGTGCTGAGTGTGAAGAGTGCTGCCTGGCCTGCCACAAGAAATGTCTGGAGACGCTGGCCATACAGTGC GGGCACAAGAAGCTGCAAGGCCGCCTGCAGCTGTTCGGCCAGGACTTCAGCCACGCGGCCCGCAGCGCCC CCGACGGCGTGCCCTTCATCGTCAAGAAGTGCGTCTGCGAGATCGAGCGGCGGGCGCTGCGCACCAAGGG CATCTACCGGGTCAATGGGGTAAAGACACGCGTGGAGAAGCTGTGCCAGGCCTTCGAGAACGGCAAGGAG CTGGTCGAGCTGTCGCAGGCCTCGCCCCACGACATCAGCAACGTCCTCAAGCTCTACCTGCGTCAGCTTC CCGAGCCGCTCATCTCCTTCCGCCTCTACCACGAGCTCGTAGGGCTGGCCAAGGACAGCCTGAAGGCAGA GGCCGAGGCCAAGGCGGCGTCCCGGGGCCGGCAGGACGGCTCGGAGAGCGAGGCAGTGGCGGTGGCCCTG GCAGGTCGGCTGCGGGAGCTCCTGCGGGACCTGCCGCCTGAGAACCGGGCCTCGCTGCAGTACCTGCTGC GTCACCTACGCAGGATCGTGGAGGTGGAGCAGGACAACAAGATGACCCCCGGGAACCTGGGCATCGTGTT CGGGCCCACGCTGCTTCGGCCACGGCCCACCGAGGCCACCGTGTCCCTCTCCTCCCTGGTGGATTATCCC CATCAGGCCCGCGTCATCGAGACTCTCATCGTCCACTACGGCCTGGTCTTCGAGGAGGAGCCGGAGGAGA CCCCCGGGGGCCAGGACGAGTCATCCAACCAGCGAGCTGAGGTAGTCGTCCAGGTGCCGTACCTGGAGGC GGGCGAGGCGGTGGTCTACCCGCTGCAGGAGGCGGCGGCGGACGGGTGCAGAGAATCCCGAGTTGTGTCC AACGATTCGGACTCGGACCTAGAGGAGGCCTCCGAGCTGCTGTCCTCATCGGAGGCCAGTGCCCTGGGCC ACCTCAGCTTCCTGGAGCAGCAGCAGAGCGAGGCCAGCCTAGAGGTGGCTTCTGGCAGCCACAGCGGCAG TGAGGAGCAGCTGGAGGCCACAGCCCGGGAGGACGGGGACGGGGACGAGGACGGCCCGGCCCAGCAGCTC TCAGGATTCAACACCAACCAGTCCAACAACGTGCTGCAGGCCCCACTGCCCCCCATGAGGCTCCGTGGCG GGCGGATGACACTGGGCTCCTGCAGGGAAAGGCAGCCGGAATTCGTGTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001282335
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282335.1, NP_001269264.1
RefSeq Size	4965 bp
RefSeq ORF	3060 bp
Locus ID	23526
UniProt ID	Q92619
Gene Summary	Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate internal promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at an alternate AUG, and the resulting isoform (4) has a shorter and distinct N-terminus compared to isoform 1.

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