HA1 (ARHGAP45) (NM_001282335) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	HA-1; HLA-HA1; HMHA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239887 representing NM_001282335 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTATCTGCGGGACGGCGCACCCGGTGCTGGACGAGGGCCCCGTGCGCTGCCGGGCGGGCCCCCGAG ACCTCCTTGAGGCCCGCCGCCCGCGGGCCCACGAGTGCCTGGGTGAGGCTCTGCGTGTCATGCATCAGAT CATCTCCAAGTACCCGCTGCTGAACACCGTGGAGACGCTCACCGCAGCCGGCACCCTCATTGCCAAGGTC AAAGCCTTCCATTATGAGAGCAACAATGATCTGGAGAAACAGGAGTTCGAGAAGGCCCTGGAGACGATTG CTGTGGCCTTCAGTAGCACAGTGTCCGAGTTCCTCATGGGTGAAGTGGACAGCAGCACCCTCCTAGCAGT GCCTCCTGGGGACTCGAGCCAGTCCATGGAAAGCCTGTATGGACCGGGCAGTGAGGGCACGCCTCCCAGC CTGGAAGACTGTGACGCCGGCTGCCTGCCCGCCGAGGAGGTGGACGTGCTGCTACAGCGCTGTGAGGGGG GCGTGGATGCCGCACTGCTGTATGCCAAGAACATGGCCAAGTACATGAAGGACCTCATCAGCTACCTGGA GAAGCGGACGACGCTGGAGATGGAGTTTGCCAAGGGCCTGCAGAAGATCGCTCACAACTGCAGACAGAGC GTCATGCAGGAGCCCCACATGCCGCTCCTGTCCATCTACTCGCTGGCCCTGGAGCAGGACCTGGAGTTCG GCCACAGCATGGTGCAGGCGGTGGGCACCTTGCAGACCCAGACCTTCATGCAGCCCCTGACCCTGCGGCG GCTTGAACACGAGAAGCGCAGGAAGGAGATCAAGGAGGCCTGGCACCGTGCCCAGAGGAAGCTGCAAGAG GCGGAGTCCAACCTGCGCAAGGCCAAGCAGGGTTACGTGCAGCGCTGCGAGGACCACGACAAGGCTCGCT TCCTCGTGGCCAAGGCGGAGGAGGAGCAGGCTGGCAGCGCGCCGGGAGCAGGCAGCACGGCCACCAAGAC CCTGGACAAGCGGCGGCGGCTGGAGGAGGAGGCCAAGAACAAGGCGGAGGAAGCTATGGCCACCTACCGC ACCTGCGTGGCCGACGCGAAGACGCAGAAGCAGGAGCTGGAGGATACCAAGGTGACGGCGCTGCGGCAGA TCCAGGAGGTCATCCGGCAGAGCGACCAAACCATCAAGTCGGCCACGATCTCCTACTACCAGATGATGCA TATGCAGACGGCGCCGCTGCCCGTGCACTTCCAGATGCTGTGTGAGAGCAGCAAGCTGTATGACCCAGGC CAGCAGTACGCCTCCCACGTGCGCCAGCTGCAGCGGGACCAGGAGCCCGATGTGCACTACGACTTTGAGC CCCACGTCTCCGCCAACGCCTGGTCCCCCGTCATGCGTGCCCGGAAGAGCAGCTTCAACGTGAGTGATGT GGCGCGGCCGGAGGCTGCCGGGAGCCCCCCAGAAGAAGGCGGGTGCACTGAGGGCACACCTGCCAAGGAC CACAGGGCCGGGCGAGGACACCAGGTTCACAAGTCATGGCCGCTCTCGATCTCAGACTCGGACAGTGGGC TGGACCCCGGCCCTGGCGCAGGGGACTTTAAGAAGTTCGAGCGGACGTCATCCAGTGGTACCATGTCGTC CACGGAGGAGCTGGTGGACCCAGACGGTGGAGCCGGGGCTTCAGCCTTTGAGCAGGCTGACCTCAACGGC ATGACCCCCGAGCTGCCGGTGGCCGTGCCCAGTGGACCGTTCCGCCACGAGGGGCTGTCCAAGGCGGCCC GTACTCACCGGCTCCGGAAGCTCCGCACGCCCGCCAAGTGCCGCGAGTGCAACAGCTACGTCTACTTCCA GGGTGCTGAGTGTGAAGAGTGCTGCCTGGCCTGCCACAAGAAATGTCTGGAGACGCTGGCCATACAGTGC GGGCACAAGAAGCTGCAAGGCCGCCTGCAGCTGTTCGGCCAGGACTTCAGCCACGCGGCCCGCAGCGCCC CCGACGGCGTGCCCTTCATCGTCAAGAAGTGCGTCTGCGAGATCGAGCGGCGGGCGCTGCGCACCAAGGG CATCTACCGGGTCAATGGGGTAAAGACACGCGTGGAGAAGCTGTGCCAGGCCTTCGAGAACGGCAAGGAG CTGGTCGAGCTGTCGCAGGCCTCGCCCCACGACATCAGCAACGTCCTCAAGCTCTACCTGCGTCAGCTTC CCGAGCCGCTCATCTCCTTCCGCCTCTACCACGAGCTCGTAGGGCTGGCCAAGGACAGCCTGAAGGCAGA GGCCGAGGCCAAGGCGGCGTCCCGGGGCCGGCAGGACGGCTCGGAGAGCGAGGCAGTGGCGGTGGCCCTG GCAGGTCGGCTGCGGGAGCTCCTGCGGGACCTGCCGCCTGAGAACCGGGCCTCGCTGCAGTACCTGCTGC GTCACCTACGCAGGATCGTGGAGGTGGAGCAGGACAACAAGATGACCCCCGGGAACCTGGGCATCGTGTT CGGGCCCACGCTGCTTCGGCCACGGCCCACCGAGGCCACCGTGTCCCTCTCCTCCCTGGTGGATTATCCC CATCAGGCCCGCGTCATCGAGACTCTCATCGTCCACTACGGCCTGGTCTTCGAGGAGGAGCCGGAGGAGA CCCCCGGGGGCCAGGACGAGTCATCCAACCAGCGAGCTGAGGTAGTCGTCCAGGTGCCGTACCTGGAGGC GGGCGAGGCGGTGGTCTACCCGCTGCAGGAGGCGGCGGCGGACGGGTGCAGAGAATCCCGAGTTGTGTCC AACGATTCGGACTCGGACCTAGAGGAGGCCTCCGAGCTGCTGTCCTCATCGGAGGCCAGTGCCCTGGGCC ACCTCAGCTTCCTGGAGCAGCAGCAGAGCGAGGCCAGCCTAGAGGTGGCTTCTGGCAGCCACAGCGGCAG TGAGGAGCAGCTGGAGGCCACAGCCCGGGAGGACGGGGACGGGGACGAGGACGGCCCGGCCCAGCAGCTC TCAGGATTCAACACCAACCAGTCCAACAACGTGCTGCAGGCCCCACTGCCCCCCATGAGGCTCCGTGGCG GGCGGATGACACTGGGCTCCTGCAGGGAAAGGCAGCCGGAATTCGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239887 representing NM_001282335 Red=Cloning site Green=Tags(s) MCICGTAHPVLDEGPVRCRAGPRDLLEARRPRAHECLGEALRVMHQIISKYPLLNTVETLTAAGTLIAKV KAFHYESNNDLEKQEFEKALETIAVAFSSTVSEFLMGEVDSSTLLAVPPGDSSQSMESLYGPGSEGTPPS LEDCDAGCLPAEEVDVLLQRCEGGVDAALLYAKNMAKYMKDLISYLEKRTTLEMEFAKGLQKIAHNCRQS VMQEPHMPLLSIYSLALEQDLEFGHSMVQAVGTLQTQTFMQPLTLRRLEHEKRRKEIKEAWHRAQRKLQE AESNLRKAKQGYVQRCEDHDKARFLVAKAEEEQAGSAPGAGSTATKTLDKRRRLEEEAKNKAEEAMATYR TCVADAKTQKQELEDTKVTALRQIQEVIRQSDQTIKSATISYYQMMHMQTAPLPVHFQMLCESSKLYDPG QQYASHVRQLQRDQEPDVHYDFEPHVSANAWSPVMRARKSSFNVSDVARPEAAGSPPEEGGCTEGTPAKD HRAGRGHQVHKSWPLSISDSDSGLDPGPGAGDFKKFERTSSSGTMSSTEELVDPDGGAGASAFEQADLNG MTPELPVAVPSGPFRHEGLSKAARTHRLRKLRTPAKCRECNSYVYFQGAECEECCLACHKKCLETLAIQC GHKKLQGRLQLFGQDFSHAARSAPDGVPFIVKKCVCEIERRALRTKGIYRVNGVKTRVEKLCQAFENGKE LVELSQASPHDISNVLKLYLRQLPEPLISFRLYHELVGLAKDSLKAEAEAKAASRGRQDGSESEAVAVAL AGRLRELLRDLPPENRASLQYLLRHLRRIVEVEQDNKMTPGNLGIVFGPTLLRPRPTEATVSLSSLVDYP HQARVIETLIVHYGLVFEEEPEETPGGQDESSNQRAEVVVQVPYLEAGEAVVYPLQEAAADGCRESRVVS NDSDSDLEEASELLSSSEASALGHLSFLEQQQSEASLEVASGSHSGSEEQLEATAREDGDGDEDGPAQQL SGFNTNQSNNVLQAPLPPMRLRGGRMTLGSCRERQPEFV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282335
ORF Size	3057 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282335.2
RefSeq Size	4965 bp
RefSeq ORF	3060 bp
Locus ID	23526
UniProt ID	Q92619
MW	112.9 kDa
Gene Summary	Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function]

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