SENP7 (NM_001282801) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282801, the custom clone sequence may differ by one or more nucleotides ATGGACAAGAGAAAGCTCGGGCGACGGCCATCTTCATCCGAAATCATCACAGAAGGAAAAAGGAAAAAGT CATCTTCTGATTTATCGGAGATAAGAAAGATGTTAAATGCAAAACCAGAGGATGTCCATGTTCAATCACC ACTGTCCAAATTCAGAAGCTCAGAACGCTGGACTCTCCCTTTGCAGTGGGAAAGAAGCCTAAGGAATAAA GTCATCTCTCTAGACCATAAAAATAAAAAACATATCCGAGGGTGTCCTGTTACTTCCAAGTCATCACCAG AAAGGATACCCAGAGTTATATTGACGAATGTCCTGGGAACGGAGTTAGGAAGAAAATACATAAGGACCCC ACCTGTAACTGAGGGAAGTTTGAGTGATACAGACAACTTGCAATCAGAGCAACTTTCTTCATCATCTGAT GGCAGCCTAGAATCTTATCAAAATCTAAACCCTCACAAGAGCTGTTATTTATCTGAAAGGGGCTCACAAC GAAGTAAGACAGTAGATGACAATTCTGCAAAGCAGACTGCGCACAATAAAGAAAAACGAAGAAAGGATGA TGGCATTTCTCTTTTAATATCTGATACTCAGCCTGAAGACCTTAACAGTGGAAGTAGAGGTTGTGATCAT CTCGAACAGGAAAGCAGAAACAAGGATGTTAAATATTCTGATTCAAAAGTGGAACTCACTCTGATTTCCA GGAAGACAAAGAGAAGGCTTAGAAATAATTTACCTGATTCTCAATATTGTACTTCTTTGGATAAGTCAAC AGAACAGACAAAAAAACAAGAAGATGACTCAACAATATCCACTGAGTTTGAAAAGCCAAGTGAAAACTAT CATCAGGATCCAAAACTGCCTGAAGAAATTACAACTAAACCTACAAAAAGTGATTTTACTAAACTATCCT CACTTAACAGTCAGGAGTTGACTTTGAGTAATGCCACCAAAAGTGCCTCTGCCGGTTCAACCACTGAAAC CGTTGAGAACTCTAATTCCATTGATATTGTGGGGATTTCTTCCCTGGTTGAGAAGGATGAGAATGAGTTG AATACCATAGAAAAGCCTATTCTAAGAGGACATAATGAAGGGAACCAATCACTGATCTCAGCTGAACCAA TTGTTGTTTCCAGTGATGAAGAAGGACCTGTTGAACATAAAAGTTCAGAAATTCTTAAGTTACAATCTAA GCAAGACCGTGAGACAACTAATGAAAATGAGAGTACTTCTGAATCAGCATTGTTAGAACTACCATTGATT ACATGTGAATCTGTACAGATGTCATCTGAATTATGCCCATATAATCCTGTCATGGAGAACATTTCCAGTA TTATGCCTAGTAATGAGATGGATCTACAACTGGATTTTATATTTACTTCTGTTTATATTGGTAAAATAAA AGGAGCTTCTAAAGGTTGTGTTACAATCACAAAAAAATATATTAAGATCCCATTTCAAGTGTCCCTGAAT GAGATTTCATTGCTAGTGGATACCACACATTTAAAGCGGTTTGGGTTATGGAAAAGTAAGGATGATAATC ACAGTAAAAGGAGTCATGCTATTCTTTTCTTCTGGGTCTCTTCAGATTATCTTCAAGAGATTCAGACCCA ATTAGAACACTCTGTATTAAGCCAGCAATCAAAATCTAGTGAATTCATTTTCCTTGAACTACACAATCCT GTTTCACAAAGAGAAGAATTGAAGCTGAAAGATATTATGACGGAAATAAGTATAATCAGTGGAGAATTAG AGCTTTCTTACCCGTTGTCTTGGGTTCAGGCATTTCCTTTGTTTCAGAACCTCTCTTCAAAAGAAAGTTC TTTTATTCATTATTACTGTGTTTCAACTTGTTCTTTCCCTGCTGGTGTTGCTGTTGCTGAAGAAATGAAG CTGAAATCAGTATCTCAGCCCTCAAACACAGATGCGGCCAAGCCTACTTACACCTTCCTGCAGAAGCAAA GTAGCGGTTGCTACTCCCTTTCTATTACATCTAATCCAGATGAAGAATGGCGAGAAGTCAGGCACACTGG ACTTGTTCAGAAGTTGATTGTATATCCTCCACCACCTACTAAGGGGGGATTAGGAGTAACTAATGAAGAT CTGGAGTGTTTAGAAGAAGGAGAGTTTCTTAATGATGTAATCATTGATTTTTACCTTAAGTATCTTATAT TGGAGAAGGCATCAGATGAACTTGTTGAACGAAGTCACATTTTTAGTAGCTTTTTCTATAAATGCTTGAC AAGAAAGGAAAATAATTTAACAGAAGATAATCCAAATCTTTCAATGGCACAGAGAAGACATAAAAGAGTA AGAACATGGACTCGTCACATAAACATTTTTAATAAAGATTACATCTTTGTACCTGTAAATGAGTCGTCTC ACTGGTATCTCGCAGTCATTTGTTTTCCATGGTTAGAAGAAGCTGTGTATGAAGATTTTCCACAAACTGT ATCCCAGCAGTCCCAGGCTCAGCAGTCCCAAAATGACAACAAAACAATAGATAATGATCTACGTACTACT TCGACACTGTCTTTGAGTGCAGAGGATTCCCAAAGTACCGAGTCGAATATGTCAGTACCAAAGAAAATGT GTAAAAGGCCATGTATTCTTATACTAGACTCCTTGAAAGCTGCTTCTGTACAAAACACAGTTCAGAATTT ACGAGAGTATTTAGAGGTAGAGTGGGAAGTTAAACTAAAAACTCATCGTCAATTCAGCAAAACAAACATG GTGGATCTATGCCCTAAAGTTCCTAAACAGGACAATAGCAGTGATTGTGGAGTATATTTATTGCAGTATG TGGAAAGCTTCTTCAAGGATCCTATTGTTAACTTTGAACTTCCAATTCATTTGGAGAAGTGGTTTCCTCG TCATGTAATAAAGACCAAACGGGAAGATATTCGAGAGCTCATCTTGAAACTTCATTTACAGCAACAGAAG GGCAGCAGTAGCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001282801
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282801.1, NP_001269730.1
RefSeq Size	4820 bp
RefSeq ORF	2955 bp
Locus ID	57337
UniProt ID	Q9BQF6
Protein Families	Druggable Genome, Protease
Gene Summary	The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for many cellular processes. SUMO-specific proteases, such as SENP7, process SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also display isopeptidase activity for deconjugation of SUMO-conjugated substrates (Lima and Reverter, 2008 [PubMed 18799455]).[supplied by OMIM, Jun 2009] Transcript Variant: This variant (3) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.