HERC4 (NM_001278185) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001278185, the custom clone sequence may differ by one or more nucleotides ATGTTGTGCTGGGGAAATGCATCCTTTGGGCAGCTAGGTTTGGGTGGAATTGATGAAGAAATTGTACTAG AGCCCAGAAAAAGTGACTTCTTTATAAATAAAAGGGTCCGAGATGTAGGATGTGGACTCAGACATACTGT GTTTGTTCTGGATGATGGAACAGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAAAAA TCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAAAATATTGTAGCTGTTTCATGTGGAGAAG CTCATACGTTAGCGCTAAATGACAAAGGCCAGGTGTATGCTTGGGGTCTCGATTCTGATGGACAGCTTGG CCTGGTAGGATCAGAGGAATGCATCAGAGTACCCAGAAATATTAAAAGTTTGTCAGATATCCAGATTGTA CAGGTTGCTTGTGGTTACTATCATTCACTTGCACTTTCTAAAGCAAGTGAAGTCTTCTGTTGGGGACAGA ATAAATATGGCCAATTGGGTTTAGGTACTGACTGTAAAAAGCAAACTTCACCGCAGCTGCTTAAGTCTTT GCTTGGAATCCCTTTCATGCAAGTTGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTTTCTGGAGCT ATCTTTGGATGGGGACGCAACAAGTTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTA ATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGTGGAGAAGATCATACTGCTGCTCT AACCAAGGAAGGTGGAGTGTTTACTTTTGGAGCTGGAGGGTATGGTCAGTTGGGCCATAATTCTACCAGT CATGAAATAAACCCAAGGAAAGTTTTTGAACTTATGGGAAGCATTGTCACTGAGATTGCTTGTGGACGGC AGCACACTTCTGCTTTTGTTCCTTCATCAGGACGAATTTACTCTTTTGGGCTTGGTGGTAATGGGCAGCT GGGAACCGGTTCAACAAGCAACAGGAAAAGCCCCTTTACTGTAAAAGGAAATTGGTACCCCTATAATGGG CAGTGTCTACCAGATATTGATTCTGAAGAATATTTCTGTGTAAAAAGAATTTTCTCAGGGGGAGATCAAA GCTTTTCACATTACTCTAGTCCCCAGAACTGTGGGCCACCAGATGACTTCAGATGTCCCAATCCGACAAA GCAGATCTGGACAGTGAATGAAGCTCTAATTCAGAAATGGCTGAGCTATCCTTCTGGAAGGTTTCCTGTG GAGATAGCCAATGAGATAGATGGAACGTTTTCTTCCTCTGGTTGCCTAAATGGAAGTTTTTTAGCTGTTA GCAATGATGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAATGCTGCTAGGCTTTTATT CCACAAACTTATACAACCTGATCATCCGCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGAATCTT ATTCCTAAACTGACTAGCTCCTTACCTGATGTTGAAGCATTGAGGTTTTATCTTACTCTACCAGAATGTC CCCTGATGAGTGATTCCAACAATTTCACAACAATAGCAATTCCCTTTGGTACAGCTCTTGTGAACCTAGA AAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGTATTCCCCCTTCTGAAAGAA GAATTTTCAACAGTTTTCTTCATACTGCATTAAAGGTTTTAGAAATACTACATAGGGTAAATGAGAAAAT GGGACAGATTATACAGTATGATAAATTTTATATACATGAAGTACAAGAATTGATAGACATAAGAAATGAT TATATCAACTGGGTCCAACAGCAGGCCTATGGAATGGATGTCAACCATGGATTAACTGAGTTGGCAGATA TCCCTGTTACAATCTGTACATATCCATTTGTATTTGATGCCCAAGCAAAAACTACTCTGTTACAGACCGA TGCAGTCTTACAGATGCAGATGGCTATTGATCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCA GTGATTGAATCTGTGAATCCCTGCTTAATTCTAGTGGTGCGTAGAGAAAATATTGTAGGAGATGCAATGG AAGTCCTTAGGAAAACAAAGAACATAGATTACAAGAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGC TGTGGATGCAGGAGGGGTGCGCAAAGAATTTTTCTTGCTCATCATGAGGGAATTATTGGATCCTAAATAC GGCATGTTTAGGTATTATGAAGATTCCAGGCTCATTTGGTTTTCTGATAAGATCACAGTTGAAAACTTTG GTGCAACAGAAGTGAAAGAGCTGGTTCTAAATGGTGCAGACACAGCTGTTAACAAACAAAATCGGCAAGA GTTTGTCGATGCTTATGTGGATTACATATTCAATAAATCAGTGGCTTCCTTATTTGATGCTTTTCATGCG GGCTTTCATAAGGTCTGTGGAGGAAAAGTCCTTCTGCTCTTTCAGCCTAATGAACTACAAGCAATGGTCA TTGGAAATACAAATTATGATTGGAAGGAACTGGAAAAGAATACAGAATACAAAGGGGAATATTGGGCAGA ACATCCTACGATAAAAATTTTTTGGGAAGTATTTCACGAATTACCATTGGAAAAGAAGAAACAGTTTCTG TTATTTTTGACAGGTAGTGATCGCATTCCTATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCAGTCCA CAGGAGGTGGTGAGGAGTATCTCCCAGTTTCCCATACTTGTTTTAATCTTCTGGATCTTCCAAAATATAC AGAAAAAGAAACTCTACGCTCTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAATATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001278185
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278185.1, NP_001265114.1
RefSeq Size	4297 bp
RefSeq ORF	2940 bp
Locus ID	26091
UniProt ID	Q5GLZ8
Protein Families	Druggable Genome
Protein Pathways	Ubiquitin mediated proteolysis
Gene Summary	HERC4 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.