VPS11 (NM_001290185) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	END1; HLD12; hVPS11; PEP5; RNF108
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290185, the custom clone sequence may differ by one or more nucleotides ATGAAATCTGTTTGTCGGAGGGGTCCGTGCCGCGCGCCTCTTTGGTTTAGCTGGTCATCCAGAGTTGTTC TGTGGTCTACGGGAAGAAAGAAGGAAGTCCATCTCCTAACTTGTTATCAACTGAGCAATCCTGGGAGGCT CCTTGACTACCCTGCACATATGGAAGGCCAGATCTGGTTCTTGCCACGTTCCCTACAGCTTACAGGCTTC CAAGCCTACAAACTACGGGTGACACACCTGTACCAACTGAAGCAGCACAATATTCTGGCATCTGTTGGAG AAGATGAAGAGGGCATCAACCCCTTGGTTAAGATCTGGAACCTGGAGAAGAGAGATGGTGGCAATCCACT CTGCACTCGAATCTTCCCTGCTATTCCAGGAACAGAGCCAACTGTTGTATCTTGTTTGACTGTCCATGAA AATCTCAACTTTATGGCCATTGGTTTCACAGATGGCAGTGTTACATTGAACAAAGGAGACATCACCCGGG ACCGGCATAGCAAGACCCAGATTTTGCACAAGGGCAACTATCCTGTAACTGGATTGGCCTTTCGCCAAGC AGGAAAGACCACTCACTTGTTTGTTGTGACAACAGAGAACGTCCAGTCCTATATAGTTTCTGGAAAAGAC TACCCTCGCGTGGAGTTGGACACCCATGGTTGTGGCCTGCGCTGCTCAGCCCTAAGTGACCCTTCTCAGG ACCTGCAGTTCATTGTGGCCGGGGATGAGTGTGTCTACTTGTACCAGCCTGATGAACGTGGGCCCTGCTT CGCCTTTGAGGGCCATAAGCTCATTGCCCACTGGTTTAGAGGCTACCTTATCATTGTCTCCCGTGACCGG AAGGTTTCTCCCAAGTCAGAGTTTACCAGCAGGGATTCACAGAGCTCCGACAAGCAGATTCTAAACATCT ATGACCTGTGCAACAAGTTCATAGCCTATAGCACCGTCTTTGAGGATGTAGTGGATGTGCTTGCTGAGTG GGGCTCCCTGTACGTGCTGACGCGGGATGGGCGGGTCCACGCACTGCAGGAGAAGGACACACAGACCAAA CTGGAGATGCTGTTTAAGAAGAACCTATTTGAGATGGCGATTAACCTTGCCAAGAGCCAGCATCTGGACA GTGATGGGCTGGCCCAGATTTTCATGCAGTATGGAGACCATCTCTACAGCAAGGGCAACCACGATGGGGC TGTCCAGCAATATATCCGAACCATTGGAAAGTTGGAGCCATCCTACGTGATCCGCAAGTTTCTGGATGCC CAGCGCATTCACAACCTGACTGCCTACCTGCAGACCCTGCACCGACAATCCCTGGCCAATGCCGACCATA CCACCCTGCTCCTCAACTGCTATACCAAGCTCAAGGACAGCTCGAAGCTGGAGGAGTTCATCAAGAAAAA GAGTGAGAGTGAAGTCCACTTTGATGTGGAGACAGCCATCAAGGTCCTCCGGCAGGCTGGCTACTACTCC CATGCCCTGTATCTGGCGGAGAACCATGCACATCATGAGTGGTACCTGAAGATCCAGCTAGAAGACATTA AGAATTATCAGGAAGCCCTTCGATACATCGGCAAGCTGCCTTTTGAGCAGGCAGAGAGCAACATGAAGCG CTACGGCAAGATCCTCATGCACCACATACCAGAGCAGACAACTCAGTTGCTGAAGGGACTTTGTACTGAT TATCGGCCCAGCCTCGAAGGCCGCAGCGATAGGGAGGCCCCAGGCTGCAGGGCCAACTCTGAGGAGTTCA TCCCCATCTTTGCCAATAACCCGCGAGAGCTGAAAGCCTTCCTAGAGCACATGAGTGAAGTGCAGCCAGA CTCACCCCAGGGGATCTACGACACACTCCTTGAGCTGCGACTGCAGAACTGGGCCCACGAGAAGGATCCA CAGGTCAAAGAGAAGCTTCACGCAGAGGCCATTTCCCTGCTGAAGAGTGGTCGCTTCTGCGACGTCTTTG ACAAGGCCCTGGTCCTGTGCCAGATGCACGACTTCCAGGATGGTGTCCTTTACCTTTATGAGCAGGGGAA GCTGTTCCAGCAGATCATGCACTACCACATGCAGCACGAGCAGTACCGGCAGGTCATCAGCGTGTGTGAG CGCCATGGGGAGCAGGACCCCTCCTTGTGGGAGCAGGCCCTCAGCTACTTCGCTCGCAAGGAGGAGGACT GCAAGGAGTATGTGGCAGCTGTCCTCAAGCATATCGAGAACAAGAACCTCATGCCACCTCTTCTAGTGGT GCAGACCCTGGCCCACAACTCCACAGCCACACTCTCCGTCATCAGGGACTACCTGGTCCAAAAACTACAG AAACAGAGCCAGCAGATTGCACAGGATGAGCTGCGGGTGCGGCGGTACCGAGAGGAGACCACCCGTATCC GCCAGGAGATCCAAGAGCTCAAGGCCAGTCCTAAGATTTTCCAAAAGACCAAGTGCAGCATCTGTAACAG TGCCTTGGAGTTGCCCTCAGTCCACTTCCTGTGTGGCCACTCCTTCCACCAACACTGCTTTGAGAGTTAC TCGGAAAGTGATGCTGACTGCCCCACCTGCCTCCCTGAAAACCGGAAGGTCATGGATATGATCCGGGCCC AGGAACAGAAACGAGATCTCCATGATCAATTCCAGCATCAGCTCAAGTGCTCCAATGACAGCTTTTCTGT GATTGCTGACTACTTTGGCAGAGGTGTTTTCAACAAATTGACTCTGCTGACCGACCCTCCCACAGCCAGA CTGACCTCCAGCCTGGAGGCTGGGCTGCAACGCGACCTACTCATGCACTCCAGGAGGGGCACTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290185
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290185.1, NP_001277114.1
RefSeq Size	3486 bp
RefSeq ORF	2796 bp
Locus ID	55823
UniProt ID	Q9H270
Protein Families	Druggable Genome
Gene Summary	Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] Transcript Variant: This variant (2) differs in its 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.

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