VPS11 (NM_001290185) Human Tagged ORF Clone
CAT#: RC239795
- TrueORF®
VPS11 (myc-DDK-tagged) - Human vacuolar protein sorting 11 homolog (S. cerevisiae) (VPS11), transcript variant 2
ORF Plasmid: tGFP
"NM_001290185" in other vectors (2)
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CNY 7,130.00
CNY 300.00
CNY 6,281.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | END1; HLD12; hVPS11; PEP5; RNF108 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC239795 representing NM_001290185
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCTGTTTGTCGGAGGGGTCCGTGCCGCGCGCCTCTTTGGTTTAGCTGGTCATCCAGAGTTGTTC TGTGGTCTACGGGAAGAAAGAAGGAAGTCCATCTCCTAACTTGTTATCAACTGAGCAATCCTGGGAGGCT CCTTGACTACCCTGCACATATGGAAGGCCAGATCTGGTTCTTGCCACGTTCCCTACAGCTTACAGGCTTC CAAGCCTACAAACTACGGGTGACACACCTGTACCAACTGAAGCAGCACAATATTCTGGCATCTGTTGGAG AAGATGAAGAGGGCATCAACCCCTTGGTTAAGATCTGGAACCTGGAGAAGAGAGATGGTGGCAATCCACT CTGCACTCGAATCTTCCCTGCTATTCCAGGAACAGAGCCAACTGTTGTATCTTGTTTGACTGTCCATGAA AATCTCAACTTTATGGCCATTGGTTTCACAGATGGCAGTGTTACATTGAACAAAGGAGACATCACCCGGG ACCGGCATAGCAAGACCCAGATTTTGCACAAGGGCAACTATCCTGTAACTGGATTGGCCTTTCGCCAAGC AGGAAAGACCACTCACTTGTTTGTTGTGACAACAGAGAACGTCCAGTCCTATATAGTTTCTGGAAAAGAC TACCCTCGCGTGGAGTTGGACACCCATGGTTGTGGCCTGCGCTGCTCAGCCCTAAGTGACCCTTCTCAGG ACCTGCAGTTCATTGTGGCCGGGGATGAGTGTGTCTACTTGTACCAGCCTGATGAACGTGGGCCCTGCTT CGCCTTTGAGGGCCATAAGCTCATTGCCCACTGGTTTAGAGGCTACCTTATCATTGTCTCCCGTGACCGG AAGGTTTCTCCCAAGTCAGAGTTTACCAGCAGGGATTCACAGAGCTCCGACAAGCAGATTCTAAACATCT ATGACCTGTGCAACAAGTTCATAGCCTATAGCACCGTCTTTGAGGATGTAGTGGATGTGCTTGCTGAGTG GGGCTCCCTGTACGTGCTGACGCGGGATGGGCGGGTCCACGCACTGCAGGAGAAGGACACACAGACCAAA CTGGAGATGCTGTTTAAGAAGAACCTATTTGAGATGGCGATTAACCTTGCCAAGAGCCAGCATCTGGACA GTGATGGGCTGGCCCAGATTTTCATGCAGTATGGAGACCATCTCTACAGCAAGGGCAACCACGATGGGGC TGTCCAGCAATATATCCGAACCATTGGAAAGTTGGAGCCATCCTACGTGATCCGCAAGTTTCTGGATGCC CAGCGCATTCACAACCTGACTGCCTACCTGCAGACCCTGCACCGACAATCCCTGGCCAATGCCGACCATA CCACCCTGCTCCTCAACTGCTATACCAAGCTCAAGGACAGCTCGAAGCTGGAGGAGTTCATCAAGAAAAA GAGTGAGAGTGAAGTCCACTTTGATGTGGAGACAGCCATCAAGGTCCTCCGGCAGGCTGGCTACTACTCC CATGCCCTGTATCTGGCGGAGAACCATGCACATCATGAGTGGTACCTGAAGATCCAGCTAGAAGACATTA AGAATTATCAGGAAGCCCTTCGATACATCGGCAAGCTGCCTTTTGAGCAGGCAGAGAGCAACATGAAGCG CTACGGCAAGATCCTCATGCACCACATACCAGAGCAGACAACTCAGTTGCTGAAGGGACTTTGTACTGAT TATCGGCCCAGCCTCGAAGGCCGCAGCGATAGGGAGGCCCCAGGCTGCAGGGCCAACTCTGAGGAGTTCA TCCCCATCTTTGCCAATAACCCGCGAGAGCTGAAAGCCTTCCTAGAGCACATGAGTGAAGTGCAGCCAGA CTCACCCCAGGGGATCTACGACACACTCCTTGAGCTGCGACTGCAGAACTGGGCCCACGAGAAGGATCCA CAGGTCAAAGAGAAGCTTCACGCAGAGGCCATTTCCCTGCTGAAGAGTGGTCGCTTCTGCGACGTCTTTG ACAAGGCCCTGGTCCTGTGCCAGATGCACGACTTCCAGGATGGTGTCCTTTACCTTTATGAGCAGGGGAA GCTGTTCCAGCAGATCATGCACTACCACATGCAGCACGAGCAGTACCGGCAGGTCATCAGCGTGTGTGAG CGCCATGGGGAGCAGGACCCCTCCTTGTGGGAGCAGGCCCTCAGCTACTTCGCTCGCAAGGAGGAGGACT GCAAGGAGTATGTGGCAGCTGTCCTCAAGCATATCGAGAACAAGAACCTCATGCCACCTCTTCTAGTGGT GCAGACCCTGGCCCACAACTCCACAGCCACACTCTCCGTCATCAGGGACTACCTGGTCCAAAAACTACAG AAACAGAGCCAGCAGATTGCACAGGATGAGCTGCGGGTGCGGCGGTACCGAGAGGAGACCACCCGTATCC GCCAGGAGATCCAAGAGCTCAAGGCCAGTCCTAAGATTTTCCAAAAGACCAAGTGCAGCATCTGTAACAG TGCCTTGGAGTTGCCCTCAGTCCACTTCCTGTGTGGCCACTCCTTCCACCAACACTGCTTTGAGAGTTAC TCGGAAAGTGATGCTGACTGCCCCACCTGCCTCCCTGAAAACCGGAAGGTCATGGATATGATCCGGGCCC AGGAACAGAAACGAGATCTCCATGATCAATTCCAGCATCAGCTCAAGTGCTCCAATGACAGCTTTTCTGT GATTGCTGACTACTTTGGCAGAGGTGTTTTCAACAAATTGACTCTGCTGACCGACCCTCCCACAGCCAGA CTGACCTCCAGCCTGGAGGCTGGGCTGCAACGCGACCTACTCATGCACTCCAGGAGGGGCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC239795 representing NM_001290185
Red=Cloning site Green=Tags(s) MKSVCRRGPCRAPLWFSWSSRVVLWSTGRKKEVHLLTCYQLSNPGRLLDYPAHMEGQIWFLPRSLQLTGF QAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEPTVVSCLTVHE NLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTENVQSYIVSGKD YPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFRGYLIIVSRDR KVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVHALQEKDTQTK LEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEPSYVIRKFLDA QRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAIKVLRQAGYYS HALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQTTQLLKGLCTD YRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDP QVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCE RHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLSVIRDYLVQKLQ KQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGHSFHQHCFESY SESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLKCSNDSFSVIADYFGRGVFNKLTLLTDPPTAR LTSSLEAGLQRDLLMHSRRGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001290185 |
ORF Size | 2793 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001290185.2 |
RefSeq Size | 3486 bp |
RefSeq ORF | 2796 bp |
Locus ID | 55823 |
UniProt ID | Q9H270 |
Protein Families | Druggable Genome |
MW | 107.8 kDa |
Gene Summary | Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014] |
Documents
Product Manuals |
FAQs |
SDS |