VPS11 (NM_001290185) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	END1; HLD12; hVPS11; PEP5; RNF108
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239795 representing NM_001290185 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAATCTGTTTGTCGGAGGGGTCCGTGCCGCGCGCCTCTTTGGTTTAGCTGGTCATCCAGAGTTGTTC TGTGGTCTACGGGAAGAAAGAAGGAAGTCCATCTCCTAACTTGTTATCAACTGAGCAATCCTGGGAGGCT CCTTGACTACCCTGCACATATGGAAGGCCAGATCTGGTTCTTGCCACGTTCCCTACAGCTTACAGGCTTC CAAGCCTACAAACTACGGGTGACACACCTGTACCAACTGAAGCAGCACAATATTCTGGCATCTGTTGGAG AAGATGAAGAGGGCATCAACCCCTTGGTTAAGATCTGGAACCTGGAGAAGAGAGATGGTGGCAATCCACT CTGCACTCGAATCTTCCCTGCTATTCCAGGAACAGAGCCAACTGTTGTATCTTGTTTGACTGTCCATGAA AATCTCAACTTTATGGCCATTGGTTTCACAGATGGCAGTGTTACATTGAACAAAGGAGACATCACCCGGG ACCGGCATAGCAAGACCCAGATTTTGCACAAGGGCAACTATCCTGTAACTGGATTGGCCTTTCGCCAAGC AGGAAAGACCACTCACTTGTTTGTTGTGACAACAGAGAACGTCCAGTCCTATATAGTTTCTGGAAAAGAC TACCCTCGCGTGGAGTTGGACACCCATGGTTGTGGCCTGCGCTGCTCAGCCCTAAGTGACCCTTCTCAGG ACCTGCAGTTCATTGTGGCCGGGGATGAGTGTGTCTACTTGTACCAGCCTGATGAACGTGGGCCCTGCTT CGCCTTTGAGGGCCATAAGCTCATTGCCCACTGGTTTAGAGGCTACCTTATCATTGTCTCCCGTGACCGG AAGGTTTCTCCCAAGTCAGAGTTTACCAGCAGGGATTCACAGAGCTCCGACAAGCAGATTCTAAACATCT ATGACCTGTGCAACAAGTTCATAGCCTATAGCACCGTCTTTGAGGATGTAGTGGATGTGCTTGCTGAGTG GGGCTCCCTGTACGTGCTGACGCGGGATGGGCGGGTCCACGCACTGCAGGAGAAGGACACACAGACCAAA CTGGAGATGCTGTTTAAGAAGAACCTATTTGAGATGGCGATTAACCTTGCCAAGAGCCAGCATCTGGACA GTGATGGGCTGGCCCAGATTTTCATGCAGTATGGAGACCATCTCTACAGCAAGGGCAACCACGATGGGGC TGTCCAGCAATATATCCGAACCATTGGAAAGTTGGAGCCATCCTACGTGATCCGCAAGTTTCTGGATGCC CAGCGCATTCACAACCTGACTGCCTACCTGCAGACCCTGCACCGACAATCCCTGGCCAATGCCGACCATA CCACCCTGCTCCTCAACTGCTATACCAAGCTCAAGGACAGCTCGAAGCTGGAGGAGTTCATCAAGAAAAA GAGTGAGAGTGAAGTCCACTTTGATGTGGAGACAGCCATCAAGGTCCTCCGGCAGGCTGGCTACTACTCC CATGCCCTGTATCTGGCGGAGAACCATGCACATCATGAGTGGTACCTGAAGATCCAGCTAGAAGACATTA AGAATTATCAGGAAGCCCTTCGATACATCGGCAAGCTGCCTTTTGAGCAGGCAGAGAGCAACATGAAGCG CTACGGCAAGATCCTCATGCACCACATACCAGAGCAGACAACTCAGTTGCTGAAGGGACTTTGTACTGAT TATCGGCCCAGCCTCGAAGGCCGCAGCGATAGGGAGGCCCCAGGCTGCAGGGCCAACTCTGAGGAGTTCA TCCCCATCTTTGCCAATAACCCGCGAGAGCTGAAAGCCTTCCTAGAGCACATGAGTGAAGTGCAGCCAGA CTCACCCCAGGGGATCTACGACACACTCCTTGAGCTGCGACTGCAGAACTGGGCCCACGAGAAGGATCCA CAGGTCAAAGAGAAGCTTCACGCAGAGGCCATTTCCCTGCTGAAGAGTGGTCGCTTCTGCGACGTCTTTG ACAAGGCCCTGGTCCTGTGCCAGATGCACGACTTCCAGGATGGTGTCCTTTACCTTTATGAGCAGGGGAA GCTGTTCCAGCAGATCATGCACTACCACATGCAGCACGAGCAGTACCGGCAGGTCATCAGCGTGTGTGAG CGCCATGGGGAGCAGGACCCCTCCTTGTGGGAGCAGGCCCTCAGCTACTTCGCTCGCAAGGAGGAGGACT GCAAGGAGTATGTGGCAGCTGTCCTCAAGCATATCGAGAACAAGAACCTCATGCCACCTCTTCTAGTGGT GCAGACCCTGGCCCACAACTCCACAGCCACACTCTCCGTCATCAGGGACTACCTGGTCCAAAAACTACAG AAACAGAGCCAGCAGATTGCACAGGATGAGCTGCGGGTGCGGCGGTACCGAGAGGAGACCACCCGTATCC GCCAGGAGATCCAAGAGCTCAAGGCCAGTCCTAAGATTTTCCAAAAGACCAAGTGCAGCATCTGTAACAG TGCCTTGGAGTTGCCCTCAGTCCACTTCCTGTGTGGCCACTCCTTCCACCAACACTGCTTTGAGAGTTAC TCGGAAAGTGATGCTGACTGCCCCACCTGCCTCCCTGAAAACCGGAAGGTCATGGATATGATCCGGGCCC AGGAACAGAAACGAGATCTCCATGATCAATTCCAGCATCAGCTCAAGTGCTCCAATGACAGCTTTTCTGT GATTGCTGACTACTTTGGCAGAGGTGTTTTCAACAAATTGACTCTGCTGACCGACCCTCCCACAGCCAGA CTGACCTCCAGCCTGGAGGCTGGGCTGCAACGCGACCTACTCATGCACTCCAGGAGGGGCACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239795 representing NM_001290185 Red=Cloning site Green=Tags(s) MKSVCRRGPCRAPLWFSWSSRVVLWSTGRKKEVHLLTCYQLSNPGRLLDYPAHMEGQIWFLPRSLQLTGF QAYKLRVTHLYQLKQHNILASVGEDEEGINPLVKIWNLEKRDGGNPLCTRIFPAIPGTEPTVVSCLTVHE NLNFMAIGFTDGSVTLNKGDITRDRHSKTQILHKGNYPVTGLAFRQAGKTTHLFVVTTENVQSYIVSGKD YPRVELDTHGCGLRCSALSDPSQDLQFIVAGDECVYLYQPDERGPCFAFEGHKLIAHWFRGYLIIVSRDR KVSPKSEFTSRDSQSSDKQILNIYDLCNKFIAYSTVFEDVVDVLAEWGSLYVLTRDGRVHALQEKDTQTK LEMLFKKNLFEMAINLAKSQHLDSDGLAQIFMQYGDHLYSKGNHDGAVQQYIRTIGKLEPSYVIRKFLDA QRIHNLTAYLQTLHRQSLANADHTTLLLNCYTKLKDSSKLEEFIKKKSESEVHFDVETAIKVLRQAGYYS HALYLAENHAHHEWYLKIQLEDIKNYQEALRYIGKLPFEQAESNMKRYGKILMHHIPEQTTQLLKGLCTD YRPSLEGRSDREAPGCRANSEEFIPIFANNPRELKAFLEHMSEVQPDSPQGIYDTLLELRLQNWAHEKDP QVKEKLHAEAISLLKSGRFCDVFDKALVLCQMHDFQDGVLYLYEQGKLFQQIMHYHMQHEQYRQVISVCE RHGEQDPSLWEQALSYFARKEEDCKEYVAAVLKHIENKNLMPPLLVVQTLAHNSTATLSVIRDYLVQKLQ KQSQQIAQDELRVRRYREETTRIRQEIQELKASPKIFQKTKCSICNSALELPSVHFLCGHSFHQHCFESY SESDADCPTCLPENRKVMDMIRAQEQKRDLHDQFQHQLKCSNDSFSVIADYFGRGVFNKLTLLTDPPTAR LTSSLEAGLQRDLLMHSRRGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290185
ORF Size	2793 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290185.2
RefSeq Size	3486 bp
RefSeq ORF	2796 bp
Locus ID	55823
UniProt ID	Q9H270
Protein Families	Druggable Genome
MW	107.8 kDa
Gene Summary	Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

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