CCDC46 (CEP112) (NM_001302891) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCDC46; MACOCO; SPGF44
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001302891, the custom clone sequence may differ by one or more nucleotides ATGGAAGTGGGAAGTGAAGAAGAAAAATGGGAGAAGCTGGATGCAGAATTTGATCACTTTGTGGTTGATA TGAAGCCCTTTGTTCTAAAATTACCTCATAGGACAGAACGGCAGAGGTGTGCTCTTTGGATTAGAAAGCT GTGCGAACCTTCAGGAACAGGTGCAGGAATAATGGGGAGGAAGAATCGGAACCTGTATGCAAAATTGTTA TTGCATATGCTTAAACGAGGTGCGCTTGAAGGCCCTTTTACACACCGACCTGAACCCGGGACACTAAAAA TTCTACCTTCATACATGTCCATCTATTTTGATGAACCAAATCCAGCACGAGCAAAAGGTTCAAGCCCAGA GGGATTACCAGCCTGGGTACTGGGTGAGCTGGAGACAAGTGAACACAAATTAAATGAATCATGGAAACTC TCTTCTGGAGAAGATAACACTTTAGTACAGTCGCCAACTGATGTCTACAGCAGGGAACAGTACACTGGGA AGCTCCGAGTGAGATCACACTCCTTGAGTCCAACTCACAGAGAAGATGGACAAAATATTACCCCAAAGAT TTGTGAAGTTTATTCGAAAAAATCTCCTGTTTCTTTGGATGATAGTGACATTGAAGCTCGCCTTAATAGT TGGAATCTTGGGCTGGACATGAAAACAAAAATGATGGAAGCTAAATTTCATGAAGAAAAGCTTAAACTGC AACAGAAACATGATGCTGATGTTCAGAAGATTTTAGAAAGAAAGAATAATGAAATAGAAGAACTGAAAAC TTTATACAGGAGTAAACAACATGAAACTGAAGAGACTATTAGAAAGCTTGAAAAGAAAGTTCAGACACTG ATACGTGACTGTCAAGTTATCAGAGAGACTAAAGAAGACCAGATTGCAGAGCTGAAAAAGATATGTGAAC AAAGTACGGAATCTCTAAATAATGACTGGGAAAAAAAGCTTCACAATGCTGTAGCAGAAATGGAACAGGA AAAGTTTGATCTTCAAAAGCAACACACTGAAAACATTCAAGAATTGCTTGAGGATACAAATGTGCGTCTG AATAAAATGGAGAGTGAATACATGGCGCAAACACAGTCCACAAACCACATGATCAAAGAACTGGAGGCCC GTGTCCAGCAGCTGACTGGAGAAGCAGAGAACAGTAATTTACAGAGGCAGAAATTAATTCAAGAAAAAGC AGAACTTGAAAGATGTTACCAGATAACGTGTAGTGAATTACAAGAAGTAAAGGCAAGGCGTAACACACTG CATAAAGAGAAGGACCATCTTGTAAATGATTATGAGCAAAACATGAAACTGTTACAAACCAAATATGATG CTGATATAAACCTTCTAAAACAAGAACATGCTCTTTCAGCTTCTAAGGCATCTAGTATGATTGAAGAATT AGAGCAGAATGTCTGTCAATTAAAACAGCAGTTACAGGAATCAGAACTTCAAAGAAAGCAACAACTAAGG GATCAAGAAAATAAGTTTCAGATGGAGAAAAGTCATTTAAAACACATCTATGAAAAAAAGGCTCATGACT TGCAGAGTGAACTTGATAAAGGAAAAGAAGATACTCAAAAGAAAATTCATAAATTTGAGGAAGCTTTGAA GGAAAAAGAGGAGCAGCTAACTCGTGTGACTGAAGTTCAGAGGTTGCAGGCCCAGCAGGCAGATGCCGCT CTGGAAGAGTTTAAGCGGCAGGTGGAACTGAACTCAGAGAAAGTCTATGCTGAAATGAAAGAGCAGATGG AAAAAGTGGAGGCAGATCTAACTAGATCCAAATCTCTTCGTGAGAAACAATCAAAGGAGTTTTTATGGCA ACTGGAGGACATCAGACAGCGGTATGAACAACAGATAGTAGAGCTGAAGCTGGAGCATGAACAGGAGAAG ACGCACCTATTACAGCAGCATAACGCAGAGAAGGATAGCCTAGTCCGAGACCATGAACGGGAAATTGAAA ACCTGGAAAAACAGCTTCGCGCTGCCAATATGGAGCACGAAAATCAAATTCAGGAGTTCAAGAAACGAGA TGCACAGGTTATTGCCGACATGGAGGCCCAGGTTCACAAGTTGAGAGAAGAATTGATCAATGTGAACTCA CAGCGGAAACAGCAGCTGGTAGAGCTTGGTCTTCTTCGTGAAGAGGAAAAGCAAAGGGCTACAAGGGAAC ATGAGATTGTCGTCAATAAACTGAAGGCTGAATCAGAAAAGATGAAAATAGAGCTGAAAAAGACTCATGC TGCTGAGACAGAGATGACACTGGAAAAGGCCAACAGCAAGCTGAAGCAGATTGAGAAGGAATACACTCAG AAGCTTGCCAAATCTTCACAGATCATAGCAGAACTTCAGACAACCATTTCCTCTCTGAAAGAAGAGAACA GCCAGCAGCAGCTTGCTGCAGAAAGGCGGCTCCAGGATGTTAGACAAAAGTTTGAAGATGAGAAGAAGCA GCTGATTAGAGATAATGACCAAGCAATCAAGGTTTTACAAGATGAATTAGAAAACCGTTCTAATCAGGTG CGATGTGCAGAGAAAAAATTACAACACAAAGAATTGGAGTCACAGGAACAGATAACTTACATACGACAAG AATATGAAACAAAATTGAAAGGATTGATGCCAGCATCCCTAAGACAAGAACTTGAAGACACCATTTCCTC CCTAAAATCACAGGTTAATTTTCTGCAAAAGAGAGCTTCCATCCTTCAGGAAGAACTGACTACATATCAA GGCAGAAGGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302891
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302891.1, NP_001289820.1
RefSeq Size	3399 bp
RefSeq ORF	2742 bp
Locus ID	201134
Gene Summary	This gene encodes a coiled-coil domain containing protein that belongs to the cell division control protein 42 effector protein family. In neurons, it localizes to the cytoplasm of dendrites and is also enriched in the nucleus where it interacts with the RNA polymerase III transcriptional repressor Maf1 to regulate gamma-aminobutyric acid A receptor surface expression. In addition, the protein has been identified as a component of the human centrosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (4) uses an alternate exon in the 5' UTR and lacks two in-frame exons in the 5' coding region compared to variant 3. The encoded isoform (c) is shorter than isoform a.

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