Angiomotin like 1 (AMOTL1) (NM_001301007) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JEAP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001301007, the custom clone sequence may differ by one or more nucleotides ATGTGGAGGGCAAAGTTGCGCCGGGGAACTTGTGAGCCTGCGGTGAAAGTTGAAGATCCTCTTTGTAACT TCCACTCCCCAAACTTCCTGAGGATCTCAGAGGTGGAAATGAGAGGTTCCGAGGATGCGGCAGCTGGAAC AGTATTGCAGCGGCTGATCCAGGAACAACTGCGGTATGGCACCCCAACCGAGAACATGAACTTGCTGGCC ATTCAGCACCAGGCCACAGGGAGTGCAGGACCAGCCCATCCTACAAACAACTTTTCTTCCACGGAAAACC TCACTCAAGAAGACCCACAAATGGTCTACCAGTCAGCACGCCAAGAACCGCAGGGTCAAGAACACCAGGT GGACAATACGGTGATGGAGAAACAGGTCCGGTCCACGCAGCCTCAGCAGAACAACGAGGAACTGCCCACT TACGAGGAGGCCAAAGCACAGTCGCAGTTCTTCAGGGGGCAGCAGCAGCAGCAACAGCAGCAGGGGGCGG TGGGCCATGGTTACTACATGGCAGGGGGCACCAGTCAGAAGTCCCGAACTGAGGGGAGGCCCACTGTGAA CCGTGCCAACAGTGGACAGGCGCATAAGGACGAGGCGCTGAAGGAACTGAAGCAGGGCCACGTCCGCTCG CTCAGCGAGAGAATCATGCAGCTGTCCCTGGAGAGGAATGGGGCCAAGCAACACCTTCCCGGCTCGGGGA ATGGAAAGGGCTTCAAAGTAGGAGGGGGGCCCTCCCCTGCCCAGCCTGCAGGTAAAGTGCTGGACCCTCG GGGTCCTCCACCTGAGTACCCCTTCAAGACCAAGCAAATGATGTCCCCAGTCAGCAAGACCCAGGAGCAC GGACTTTTTTATGGTGACCAGCACCCCGGGATGCTCCACGAGATGGTCAAGCCCTACCCTGCTCCTCAGC CTGTGAGAACAGATGTGGCCGTCCTGCGGTACCAGCCACCCCCTGAGTATGGGGTAACGAGCCGCCCATG CCAACTTCCGTTCCCATCAACCATGCAGCAGCACAGCCCCATGTCCTCCCAGACCTCTTCCGCCAGCGGG CCACTGCACTCTGTCTCCCTGCCGCTTCCACTCCCGATGGCCCTGGGTGCTCCACAGCCCCCGCCTGCCG CCTCCCCCAGCCAGCAGCTTGGTCCAGATGCCTTTGCGATTGTGGAGCGAGCCCAGCAAATGGTGGAGAT ATTAACAGAGGAGAACCGGGTGCTTCACCAGGAACTTCAGGGTTACTACGACAATGCCGACAAGCTCCAC AAGTTTGAAAAAGAACTTCAGAGAATTTCGGAAGCCTATGAAAGTCTGGTCAAGTCTACCACCAAGCGAG AATCGCTGGACAAGGCCATGAGAAACAAATTGGAAGGCGAGATTAGAAGACTTCATGATTTCAACAGAGA CCTCCGAGATCGACTAGAGACTGCTAACAGGCAACTATCCAGCAGGGAATACGAAGGGCATGAAGACAAA GCTGCAGAGGGGCATTATGCTTCCCAGAACAAAGAATTCTTGAAGGAAAAGGAGAAATTAGAAATGGAGT TAGCAGCAGTGCGGACTGCAAGTGAGGACCATCGGAGACACATCGAGATCCTGGACCAGGCTTTGAGCAA CGCCCAGGCCAGGGTCATCAAGCTGGAAGAGGAGTTACGAGAGAAGCAAGCATATGTTGAGAAAGTTGAG AAGCTGCAGCAGGCCCTGACCCAGCTGCAGTCTGCATGTGAGAAGCGAGAACAGATGGAGCGGAGACTGC GGACTTGGCTGGAGAGAGAGCTGGATGCACTGAGAACCCAGCAGAAACATGGAAATGGCCAGCCAGCCAA CATGCCGGAATACAATGCCCCAGCCCTCCTGGAACTTGTGCGGGAGAAGGAGGAGCGGATCCTGGCCCTG GAGGCCGACATGACAAAGTGGGAGCAGAAGTACCTGGAGGAGAGCACCATCCGACACTTTGCCATGAATG CCGCAGCCACTGCAGCAGCTGAGAGGGACACCACGATCATCAACCACTCACGGAATGGCAGCTACGGAGA GAGCTCGCTGGAGGCCCACATCTGGCAAGAGGAGGAGGAGGTGGTGCAGGCCAACAGAAGGTGTCAGGAC ATGGAATACACTATTAAAAATCTCCATGCCAAAATCATAGAGAAAGATGCTATGATTAAGGTCCTGCAGC AGCGATCTCGTAAAGATGCCGGGAAGACAGACTCCTCCAGCCTACGTCCTGCCCGCTCCGTTCCATCCAT AGCAGCAGCTACTGGGACACACTCTCGCCAGACCTCTCTTACCAGCAGCCAGCTGGCTGAGGAAAAGAAG GAAGAGAAGACCTGGAAGGGGAGCATAGGATTGCTGCTGGGGAAGGAGCACCATGAGCATGCCTCTGCCC CACTGCTGCCACCCCCACCCACCTCAGCACTGTCCTCCATAGCCTCCACTACGGCAGCCAGCAGTGCCCA CGCCAAGACAGGCAGCAAGGACAGCAGCACACAGACTGACAAGAGTGCCGAGCTCTTCTGGCCCAGCATG GCCTCCCTTCCCAGCCGCGGCCGGCTGAGCACGACCCCTGCTCACAGCCCCGTCCTGAAACACCCAGCGG CCAAAGGGACCGCAGAGAAACTGGAGAACTCTCCTGGCCATGGGAAGTCGCCTGACCACAGAGGCCGGGT CAGCAGCTTGCTGCACAAGCCCGAGTTCCCTGATGGAGAGATGATGGAAGTCCTCATCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001301007
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301007.1, NP_001287936.1
RefSeq Size	8849 bp
RefSeq ORF	2721 bp
Locus ID	154810
UniProt ID	Q8IY63
Protein Pathways	Tight junction
Gene Summary	The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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