Estrogen induced gene 121 protein (KIAA1324) (NM_001284352) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EIG121; KIAA1324
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284352, the custom clone sequence may differ by one or more nucleotides ATGAAGGACCAGTCATGTAAGCCATGCGCTGAGGGCCGCTACTCCCTCGGCACAGGCATTCGGTTTGATG AGTGGGATGAGCTGCCCCATGGCTTTGCCAGCCTCTCAGCCAACATGGAGCTGGATGACAGTGCTGCTGA GTCCACCGGGAACTGTACTTCGTCCAAGTGGGTTCCCCGGGGCGACTACATCGCCTCCAACACGGACGAA TGCACAGCCACACTGATGTACGCCGTCAACCTGAAGCAATCTGGCACCGTTAACTTCGAATACTACTATC CAGACTCCAGCATCATCTTTGAGTTTTTCGTTCAGAATGACCAGTGCCAGCCCAATGCAGATGACTCCAG GTGGATGAAGACCACAGAGAAAGGATGGGAATTCCACAGTGTGGAGCTAAATCGAGGCAATAATGTCCTC TATTGGAGAACCACAGCCTTCTCAGTATGGACCAAAGTACCCAAGCCTGTGCTGGTGAGAAACATTGCCA TAACAGGGGTGGCCTACACTTCAGAATGCTTCCCCTGCAAACCTGGCACGTATGCAGACAAGCAGGGCTC CTCTTTCTGCAAACTTTGCCCAGCCAACTCTTATTCAAATAAAGGAGAAACTTCTTGCCACCAGTGTGAC CCTGACAAATACTCAGAGAAAGGATCTTCTTCCTGTAACGTGCGCCCAGCTTGCACAGACAAAGATTATT TCTACACACACACGGCCTGCGATGCCAACGGAGAGACACAACTCATGTACAAATGGGCCAAGCCGAAAAT CTGTAGCGAGGACCTTGAGGGGGCAGTGAAGCTGCCTGCCTCTGGTGTGAAGACCCACTGCCCACCCTGC AACCCAGGCTTCTTCAAAACCAACAACAGCACCTGCCAGCCCTGCCCATATGGTTCCTACTCCAATGGCT CAGACTGTACCCGCTGCCCTGCAGGGACTGAACCTGCTGTGGGATTTGAATACAAATGGTGGAACACGCT GCCCACAAACATGGAAACGACCGTTCTCAGTGGGATCAACTTCGAGTACAAGGGCATGACAGGCTGGGAG GTGGCTGGTGATCACATTTACACAGCTGCTGGAGCCTCAGACAATGACTTCATGATTCTCACTCTGGTTG TGCCAGGATTTAGACCTCCGCAGTCGGTGATGGCAGACACAGAGAATAAAGAGGTGGCCAGAATCACATT TGTCTTTGAGACCCTCTGTTCTGTGAACTGTGAGCTCTACTTCATGGTGGGTGTGAATTCTAGGACCAAC ACTCCTGTGGAGACGTGGAAAGGTTCCAAAGGCAAACAGTCCTATACCTACATCATTGAGGAGAACACTA CCACGAGCTTCACCTGGGCCTTCCAGAGGACCACTTTTCATGAGGCAAGCAGGAAGTACACCAATGACGT TGCCAAGATCTACTCCATCAATGTCACCAATGTTATGAATGGTGTGGCCTCCTACTGCCGTCCCTGTGCC CTAGAAGCCTCTGATGTGGGCTCCTCCTGCACCTCTTGTCCTGCTGGTTACTATATTGACCGAGATTCAG GAACCTGCCACTCCTGCCCCACTAACACAATTCTGAAAGCCCACCAGCCTTATGGTGTCCAGGCCTGTGT GCCCTGTGGTCCAGGGACCAAGAACAACAAGATCCACTCTCTGTGCTACAACGATTGCACCTTCTCACGC AACACTCCGACCAGGACTTTCAACTACAACTTCTCCGCTTTGGCAAACACTGTCACTCTTGCTGGAGGGC CAAGCTTCACTTCCAAAGGGCTGAAATACTTCCATCACTTTACCCTCAGTCTCTGTGGAAACCAGGGTAG GAAAATGTCTGTGTGCACCGACAATGTCACTGACCTCCGGATTCCTGAGGGTGAGTCAGGGTTCTCCAAA TCTATCACAGCCTACGTCTGCCAGGCAGTCATCATCCCCCCAGAGGTGACAGGCTACAAGGCCGGGGTTT CCTCACAGCCTGTCAGCCTTGCTGATCGACTTATTGGGGTGACAACAGATATGACTCTGGATGGAATCAC CTCCCCAGCTGAACTTTTCCACCTGGAGTCCTTGGGAATACCGGACGTGATCTTCTTTTATAGGTCCAAT GATGTGACCCAGTCCTGCAGTTCTGGGAGATCAACCACCATCCGCGTCAGGTGCAGTCCACAGAAAACTG TCCCTGGAAGTTTGCTGCTGCCAGGAACGTGCTCGGATGGGACCTGTGATGGCTGCAACTTCCACTTCCT GTGGGAGAGCGCGGCTGCTTGCCCGCTCTGCTCAGTGGCTGACTACCATGCTATCGTCAGCAGCTGTGTG GCTGGGATCCAGAAGACTACTTACGTGTGGCGAGAACCCAAGCTATGCTCTGGTGGCATTTCTCTGCCTG AGCAGAGAGTCACCATCTGCAAAACCATAGATTTCTGGCTGAAAGTGGGCATCTCTGCAGGCACCTGTAC TGCCATCCTGCTCACCGTCTTGACCTGCTACTTTTGGAAAAAGAATCAAAAACTAGAGTACAAGTACTCC AAGCTGGTGATGAATGCTACTCTCAAGGACTGTGACCTGCCAGCAGCTGACAGCTGCGCCATCATGGAAG GCGAGGATGTAGAGGACGACCTCATCTTTACCAGCAAGAAGTCACTCTTTGGGAAGATCAAATCATTTAC CTCCAAGCAGCCAGCTCCTGTCACCATCTCTCTTTCAGAGGACTCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001284352
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284352.1, NP_001271281.1
RefSeq Size	6964 bp
RefSeq ORF	2709 bp
Locus ID	57535
Protein Families	Transmembrane
Gene Summary	Expression of this gene is induced by estrogen and the encoded protein has been characterized as a transmembrane protein. The encoded protein has been found in to correlate with survival in certain carcinomas (PMID: 21102415) and may be important for cellular response to stress (PMID: 21072319). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012] Transcript Variant: This variant (4) uses an alternate 5' exon structure and thus differs in the 5' UTR and 5' coding region, and also uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. These differences cause translation initiation at a downstream start codon and result in an isoform (4) with a shorter N-terminus and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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