Estrogen induced gene 121 protein (KIAA1324) (NM_001284352) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EIG121; KIAA1324
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239756 representing NM_001284352 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACCAGTCATGTAAGCCATGCGCTGAGGGCCGCTACTCCCTCGGCACAGGCATTCGGTTTGATG AGTGGGATGAGCTGCCCCATGGCTTTGCCAGCCTCTCAGCCAACATGGAGCTGGATGACAGTGCTGCTGA GTCCACCGGGAACTGTACTTCGTCCAAGTGGGTTCCCCGGGGCGACTACATCGCCTCCAACACGGACGAA TGCACAGCCACACTGATGTACGCCGTCAACCTGAAGCAATCTGGCACCGTTAACTTCGAATACTACTATC CAGACTCCAGCATCATCTTTGAGTTTTTCGTTCAGAATGACCAGTGCCAGCCCAATGCAGATGACTCCAG GTGGATGAAGACCACAGAGAAAGGATGGGAATTCCACAGTGTGGAGCTAAATCGAGGCAATAATGTCCTC TATTGGAGAACCACAGCCTTCTCAGTATGGACCAAAGTACCCAAGCCTGTGCTGGTGAGAAACATTGCCA TAACAGGGGTGGCCTACACTTCAGAATGCTTCCCCTGCAAACCTGGCACGTATGCAGACAAGCAGGGCTC CTCTTTCTGCAAACTTTGCCCAGCCAACTCTTATTCAAATAAAGGAGAAACTTCTTGCCACCAGTGTGAC CCTGACAAATACTCAGAGAAAGGATCTTCTTCCTGTAACGTGCGCCCAGCTTGCACAGACAAAGATTATT TCTACACACACACGGCCTGCGATGCCAACGGAGAGACACAACTCATGTACAAATGGGCCAAGCCGAAAAT CTGTAGCGAGGACCTTGAGGGGGCAGTGAAGCTGCCTGCCTCTGGTGTGAAGACCCACTGCCCACCCTGC AACCCAGGCTTCTTCAAAACCAACAACAGCACCTGCCAGCCCTGCCCATATGGTTCCTACTCCAATGGCT CAGACTGTACCCGCTGCCCTGCAGGGACTGAACCTGCTGTGGGATTTGAATACAAATGGTGGAACACGCT GCCCACAAACATGGAAACGACCGTTCTCAGTGGGATCAACTTCGAGTACAAGGGCATGACAGGCTGGGAG GTGGCTGGTGATCACATTTACACAGCTGCTGGAGCCTCAGACAATGACTTCATGATTCTCACTCTGGTTG TGCCAGGATTTAGACCTCCGCAGTCGGTGATGGCAGACACAGAGAATAAAGAGGTGGCCAGAATCACATT TGTCTTTGAGACCCTCTGTTCTGTGAACTGTGAGCTCTACTTCATGGTGGGTGTGAATTCTAGGACCAAC ACTCCTGTGGAGACGTGGAAAGGTTCCAAAGGCAAACAGTCCTATACCTACATCATTGAGGAGAACACTA CCACGAGCTTCACCTGGGCCTTCCAGAGGACCACTTTTCATGAGGCAAGCAGGAAGTACACCAATGACGT TGCCAAGATCTACTCCATCAATGTCACCAATGTTATGAATGGTGTGGCCTCCTACTGCCGTCCCTGTGCC CTAGAAGCCTCTGATGTGGGCTCCTCCTGCACCTCTTGTCCTGCTGGTTACTATATTGACCGAGATTCAG GAACCTGCCACTCCTGCCCCACTAACACAATTCTGAAAGCCCACCAGCCTTATGGTGTCCAGGCCTGTGT GCCCTGTGGTCCAGGGACCAAGAACAACAAGATCCACTCTCTGTGCTACAACGATTGCACCTTCTCACGC AACACTCCGACCAGGACTTTCAACTACAACTTCTCCGCTTTGGCAAACACTGTCACTCTTGCTGGAGGGC CAAGCTTCACTTCCAAAGGGCTGAAATACTTCCATCACTTTACCCTCAGTCTCTGTGGAAACCAGGGTAG GAAAATGTCTGTGTGCACCGACAATGTCACTGACCTCCGGATTCCTGAGGGTGAGTCAGGGTTCTCCAAA TCTATCACAGCCTACGTCTGCCAGGCAGTCATCATCCCCCCAGAGGTGACAGGCTACAAGGCCGGGGTTT CCTCACAGCCTGTCAGCCTTGCTGATCGACTTATTGGGGTGACAACAGATATGACTCTGGATGGAATCAC CTCCCCAGCTGAACTTTTCCACCTGGAGTCCTTGGGAATACCGGACGTGATCTTCTTTTATAGGTCCAAT GATGTGACCCAGTCCTGCAGTTCTGGGAGATCAACCACCATCCGCGTCAGGTGCAGTCCACAGAAAACTG TCCCTGGAAGTTTGCTGCTGCCAGGAACGTGCTCGGATGGGACCTGTGATGGCTGCAACTTCCACTTCCT GTGGGAGAGCGCGGCTGCTTGCCCGCTCTGCTCAGTGGCTGACTACCATGCTATCGTCAGCAGCTGTGTG GCTGGGATCCAGAAGACTACTTACGTGTGGCGAGAACCCAAGCTATGCTCTGGTGGCATTTCTCTGCCTG AGCAGAGAGTCACCATCTGCAAAACCATAGATTTCTGGCTGAAAGTGGGCATCTCTGCAGGCACCTGTAC TGCCATCCTGCTCACCGTCTTGACCTGCTACTTTTGGAAAAAGAATCAAAAACTAGAGTACAAGTACTCC AAGCTGGTGATGAATGCTACTCTCAAGGACTGTGACCTGCCAGCAGCTGACAGCTGCGCCATCATGGAAG GCGAGGATGTAGAGGACGACCTCATCTTTACCAGCAAGAAGTCACTCTTTGGGAAGATCAAATCATTTAC CTCCAAGCAGCCAGCTCCTGTCACCATCTCTCTTTCAGAGGACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239756 representing NM_001284352 Red=Cloning site Green=Tags(s) MKDQSCKPCAEGRYSLGTGIRFDEWDELPHGFASLSANMELDDSAAESTGNCTSSKWVPRGDYIASNTDE CTATLMYAVNLKQSGTVNFEYYYPDSSIIFEFFVQNDQCQPNADDSRWMKTTEKGWEFHSVELNRGNNVL YWRTTAFSVWTKVPKPVLVRNIAITGVAYTSECFPCKPGTYADKQGSSFCKLCPANSYSNKGETSCHQCD PDKYSEKGSSSCNVRPACTDKDYFYTHTACDANGETQLMYKWAKPKICSEDLEGAVKLPASGVKTHCPPC NPGFFKTNNSTCQPCPYGSYSNGSDCTRCPAGTEPAVGFEYKWWNTLPTNMETTVLSGINFEYKGMTGWE VAGDHIYTAAGASDNDFMILTLVVPGFRPPQSVMADTENKEVARITFVFETLCSVNCELYFMVGVNSRTN TPVETWKGSKGKQSYTYIIEENTTTSFTWAFQRTTFHEASRKYTNDVAKIYSINVTNVMNGVASYCRPCA LEASDVGSSCTSCPAGYYIDRDSGTCHSCPTNTILKAHQPYGVQACVPCGPGTKNNKIHSLCYNDCTFSR NTPTRTFNYNFSALANTVTLAGGPSFTSKGLKYFHHFTLSLCGNQGRKMSVCTDNVTDLRIPEGESGFSK SITAYVCQAVIIPPEVTGYKAGVSSQPVSLADRLIGVTTDMTLDGITSPAELFHLESLGIPDVIFFYRSN DVTQSCSSGRSTTIRVRCSPQKTVPGSLLLPGTCSDGTCDGCNFHFLWESAAACPLCSVADYHAIVSSCV AGIQKTTYVWREPKLCSGGISLPEQRVTICKTIDFWLKVGISAGTCTAILLTVLTCYFWKKNQKLEYKYS KLVMNATLKDCDLPAADSCAIMEGEDVEDDLIFTSKKSLFGKIKSFTSKQPAPVTISLSEDS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001284352
ORF Size	2706 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001284352.2
RefSeq Size	6964 bp
RefSeq ORF	2709 bp
Locus ID	57535
Protein Families	Transmembrane
MW	99.5 kDa
Gene Summary	Expression of this gene is induced by estrogen and the encoded protein has been characterized as a transmembrane protein. The encoded protein has been found in to correlate with survival in certain carcinomas (PMID: 21102415) and may be important for cellular response to stress (PMID: 21072319). Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

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