DNA Ligase I (LIG1) (NM_001289063) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289063, the custom clone sequence may differ by one or more nucleotides ATGCAGCGAAGTATCATGGCGGCACTGAAGGAGTGGAATGGAGTGGTGTCCGAGAGTGACTCTCCGGTGA AGAGGCCAGGGAGGAAGGCGGCCCGGGTCCTGGGCAGCGAAGGGGAAGAGGAGGATGAAGCCCTTAGCCC TGCTAAAGGCCAGAAGCCTGCCCTGGACTGCTCACAGGTCTCCCCGCCCCGTCCTGCCACATCTCCTGAG AACAATGCTTCCCTCTCTGACACCTCTCCCATGGACAGTTCCCCATCAGGGATTCCGAAGCGTCGCACAG CTCGGAAGCAGCTCCCGAAACGGACCATTCAGGAAGTCCTGGAAGAGCAGAGTGAGGACGAGGACAGAGA AGCCAAGAGGAAGAAGGAGGAGGAAGAGGAGACCCCGAAAGAAAGCCTCACAGAGGCTGAAGTGGCCACA GAGAAGGAAGGAGAAGACGGGGACCAGCCCACCACGCCTCCCAAGCCCCTAAAGACCTCCAAAGCAGAGA CCCCGACGGAAAGCGTTTCAGAGCCTGAGGTGGCCACGAAGCAGGAACTGCAGGAGGAGGAAGAGCAGAC CAAGCCTCCCCGCAGAGCTCCCAAGACGCTCAGCAGCTTCTTCACCCCCCGGAAGCCAGCAGTCAAAAAA GAAGTGAAGGAAGAGGAGCCAGGGGCTCCAGGAAAGGAGGGAGCTGCTGAGGGACCCCTGGATCCATCTG GTTACAATCCTGCCAAGAACAACTATCATCCCGTGGAAGATGCCTGCTGGAAACCGGGCCAGAAGGTTCC TTACCTGGCTGTGGCCCGGACGTTTGAGAAGATCGAGGAGGTGTCTGCTCGGCTCCGGATGGTGGAGACG CTGAGCAACTTGCTGCGCTCCGTGGTGGCCCTGTCGCCTCCAGACCTCCTCCCTGTCCTCTACCTCAGCC TCAACCACCTTGGGCCACCCCAGCAGGGCCTGGAGCTTGGCGTGGGTGATGGTGTCCTTCTCAAGGCAGT GGCCCAGGCCACAGGTCGGCAGCTGGAGTCCGTCCGGGCTGAGGCAGCCGAGAAAGGCGACGTGGGGCTG GTGGCCGAGAACAGCCGCAGCACCCAGAGGCTCATGCTGCCACCACCTCCGCTCACTGCCTCCGGGGTCT TCAGCAAGTTCCGCGACATCGCCAGGCTCACTGGCAGTGCTTCCACAGCCAAGAAGATAGACATCATCAA AGGCCTCTTTGTGGCCTGCCGCCACTCAGAAGCCCGGTTCATCGCTAGGTCCCTGAGCGGACGGCTGCGC CTTGGGCTGGCAGAGCAGTCGGTGCTGGCTGCCCTCTCCCAGGCAGTGAGCCTCACGCCCCCGGGCCAAG AATTCCCACCAGCCATGGTGGATGCTGGGAAGGGCAAGACAGCAGAGGCCAGAAAGACGTGGCTGGAGGA GCAAGGCATGATCCTGAAGCAGACGTTCTGCGAGGTTCCCGACCTGGACCGAATTATCCCCGTGCTGCTG GAGCACGGCCTGGAACGTCTCCCGGAGCACTGCAAGCTGAGCCCAGGGATTCCCCTGAAACCAATGTTGG CCCATCCCACCCGGGGCATCAGCGAGGTCCTGAAACGCTTTGAGGAGGCAGCTTTCACCTGCGAATACAA ATATGACGGGCAGAGGGCACAGATCCACGCCCTGGAAGGCGGGGAGGTGAAGATCTTCAGCAGGAATCAG GAAGACAACACTGGGAAGTACCCGGACATCATCAGCCGCATCCCCAAGATTAAACTCCCATCGGTCACAT CCTTCATCCTGGACACCGAAGCCGTGGCTTGGGACCGGGAAAAGAAGCAGATCCAGCCATTCCAAGTGCT CACCACCCGCAAACGCAAGGAGGTGGATGCGTCTGAGATCCAGGTGCAGGTGTGTTTGTACGCCTTCGAC CTCATCTACCTCAATGGAGAGTCCCTGGTACGTGAGCCCCTTTCCCGGCGCCGGCAGCTGCTCCGGGAGA ACTTTGTGGAGACAGAGGGCGAGTTTGTCTTCGCCACCTCCCTGGACACCAAGGACATCGAGCAGATCGC CGAGTTCCTGGAGCAGTCAGTGAAAGACTCCTGCGAGGGGCTGATGGTGAAGACCCTGGATGTTGATGCC ACCTACGAGATCGCCAAGAGATCGCACAACTGGCTCAAGCTGAAGAAGGACTACCTTGATGGCGTGGGTG ACACCCTGGACCTGGTGGTGATCGGCGCCTACCTGGGCCGGGGGAAGCGGGCCGGCCGGTACGGGGGCTT CCTGCTGGCCTCCTACGACGAGGACAGTGAGGAGCTGCAGGCCATATGCAAGCTTGGAACTGGCTTCAGT GATGAGGAGCTGGAGGAGCATCACCAGAGCCTCAAGGCGCTGGTGCTGCCCAGCCCACGCCCTTACGTGC GGATAGATGGCGCTGTGATTCCCGACCACTGGCTGGACCCCAGCGCTGTGTGGGAGGTGAAGTGCGCTGA CCTCTCCCTCTCTCCCATCTACCCTGCTGCGCGGGGCCTGGTGGATAGTGACAAGGGCATCTCCCTTCGC TTCCCTCGGTTTATTCGAGTCCGTGAAGACAAGCAGCCGGAGCAGGCCACCACCAGTGCTCAGGTGGCCT GTTTGTACCGGAAGCAAAGTCAGATTCAGAACCAACAAGGCGAGGACTCAGGCTCTGACCCTGAAGATAC CTACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289063
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289063.1, NP_001275992.1
RefSeq Size	3317 bp
RefSeq ORF	2667 bp
Locus ID	3978
UniProt ID	P18858
Protein Families	Druggable Genome
Protein Pathways	Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair
Gene Summary	This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

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