MTR (NM_001291940) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	cblG; HMAG; MS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001291940, the custom clone sequence may differ by one or more nucleotides ATGGGAGCCCAGGTGTTGGATGTCAACATGGATGATGGCATGCTAGATGGTCCAAGTGCAATGACCAGAT TTTGCAACTTAATTGCTTCCGAGCCAGACATCGCAAAGGTACCTTTGTGCATCGACTCCTCCAATTTTGC TGTGATTGAAGCTGGGTTAAAGTGCTGCCAAGGGAAGTGCATTGTCAATAGCATTAGTCTGAAGGAAGGA GAGGACGACTTCTTGGAGAAGGCCAGGAAGATTAAAAAGTATGGAGCTGCTATGGTGGTCATGGCTTTTG ATGAAGAAGGACAGGCAACAGAAACAGACACAAAAATCAGAGTGTGCACCCGGGCCTACCATCTGCTTGT GAAAAAACTGGGCTTTAATCCAAATGACATTATTTTTGACCCTAATATCCTAACCATTGGGACTGGAATG GAGGAACACAACTTGTATGCCATTAATTTTATCCATGCAACAAAAGTCATTAAAGAAACATTACCTGGAG CCAGAATAAGTGGAGGTCTTTCCAACTTGTCCTTCTCCTTCCGAGGAATGGAAGCCATTCGAGAAGCAAT GCATGGGGTTTTCCTTTACCATGCAATCAAGTCTGGCATGGACATGGGGATAGTGAATGCTGGAAACCTC CCTGTGTATGATGATATCCATAAGGAACTTCTGCAGCTCTGTGAAGATCTCATCTGGAATAAAGACCCTG AGGCCACTGAGAAGCTCTTACGTTATGCCCAGACTCAAGGCACAGGAGGGAAGAAAGTCATTCAGACTGA TGAGTGGAGAAATGGCCCTGTCGAAGAACGCCTTGAGTATGCCCTTGTGAAGGGCATTGAAAAACATATT ATTGAGGATACTGAGGAAGCCAGGTTAAACCAAAAAAAATATCCCCGACCTCTCAATATAATTGAAGGAC CCCTGATGAATGGAATGAAAATTGTTGGTGATCTTTTTGGAGCTGGAAAAATGTTTCTACCTCAGGTTAT AAAGTCAGCCCGGGTTATGAAGAAGGCTGTTGGCCACCTTATCCCTTTCATGGAAAAAGAAAGAGAAGAA ACCAGAGTGCTTAACGGCACAGTAGAAGAAGAGGACCCTTACCAGGGCACCATCGTGCTGGCCACTGTTA AAGGCGACGTGCACGACATAGGCAAGAACATAGTTGGAGTAGTCCTTGGCTGCAATAATTTCCGAGTTAT TGATTTAGGAGTCATGACTCCATGTGATAAGATACTGAAAGCTGCTCTTGACCACAAAGCAGATATAATT GGCCTGTCAGGACTCATCACTCCTTCCCTGGATGAAATGATTTTTGTTGCCAAGGAAATGGAGAGATTAG CTATAAGGATTCCATTGTTGATTGGAGGAGCAACCACTTCAAAAACCCACACAGCAGTTAAAATAGCTCC GAGATACAGTGCACCTGTAATCCATGTCCTGGACGCGTCCAAGAGTGTGGTGGTGTGTTCCCAGCTGTTA GATGAAAATCTAAAGGATGAATACTTTGAGGAAATCATGGAAGAATATGAAGATATTAGACAGGACCATT ATGAGTCTCTCAAGGAGAGGAGATACTTACCCTTAAGTCAAGCCAGAAAAAGTGGTTTCCAAATGGATTG GCTGTCTGAACCTCACCCAGTGAAGCCCACGTTTATTGGGACCCAGGTCTTTGAAGACTATGACCTGCAG AAGCTGGTGGACTACATTGACTGGAAGCCTTTCTTTGATGTCTGGCAGCTCCGGGGCAAGTACCCGAATC GAGGCTTTCCCAAGATATTTAACGACAAAACAGTAGGTGGAGAGGCCAGGAAGGTCTACGATGATGCCCA CAATATGCTGAACACACTGATTAGTCAAAAGAAACTCCGGGCCCGGGGTGTGGTTGGGTTCTGGCCAGCA CAGAGTATCCAAGACGACATTCACCTGTACGCAGAGGCTGCTGTGCCCCAGGCTGCAGAGCCCATAGCCA CCTTCTATGGGTTAAGGCAACAGGCTGAGAAGGACTCTGCCAGCACGGAGCCATACTACTGCCTCTCAGA CTTCATCGCTCCCTTGCATTCTGGCATCCGTGACTACCTGGGCCTGTTTGCCGTTGCCTGCTTTGGGGTA GAAGAGCTGAGCAAGGCCTATGAGGATGATGGTGACGACTACAGCAGCATCATGGTCAAGGCGCTGGGGG ACCGGCTGGCAGAGGCCTTTGCAGAAGAGCTCCATGAAAGAGTTCGCCGAGAACTGTGGGCCTACTGTGG CAGTGAGCAGCTGGACGTCGCAGACCTGCGCAGGCTGCGGTACAAGGGCATCCGCCCGGCTCCTGGCTAC CCCAGCCAGCCCGACCACACCGAGAAGCTCACCATGTGGAGACTCGCAGACATCGAGCAGTCTACAGGCA TTAGGTTAACAGAATCATTAGCAATGGCACCTGCTTCAGCAGTCTCAGGCCTCTACTTCTCCAATTTGAA GTCCAAATATTTTGCTGTGGGGAAGATTTCCAAGGATCAGGTTGAGGATTATGCATTGAGGAAGAACATA TCTGTGGCTGAGGTTGAGAAATGGCTTGGACCCATTTTGGGATATGATACAGACTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001291940
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291940.1, NP_001278869.1
RefSeq Size	10462 bp
RefSeq ORF	2577 bp
Locus ID	4548
UniProt ID	Q99707
Protein Families	Druggable Genome
Protein Pathways	Cysteine and methionine metabolism, Metabolic pathways, One carbon pool by folate
Gene Summary	This gene encodes the 5-methyltetrahydrofolate-homocysteine methyltransferase. This enzyme, also known as cobalamin-dependent methionine synthase, catalyzes the final step in methionine biosynthesis. Mutations in MTR have been identified as the underlying cause of methylcobalamin deficiency complementation group G. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014] Transcript Variant: This variant (3) lacks an internal exon, which causes translation initiation at a downstream AUG, compared to variant 1. The resulting isoform (3) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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