RNF190 (MARCH10) (NM_001288779) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MARCH-X; MARCH10; RNF190
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288779, the custom clone sequence may differ by one or more nucleotides ATGTTGCATGACGCAAGGGACAGGCAGAAGTTCTTCAGCGATGTTCAGTATCTGCGGGACATGCAGCATA AGGTGGACTCTGAGTATCAGGCTTGTCTGAGACGACAGGAATATAGAAGAGACCCAAATGAGAAGAAACG CGATCAGTTTTGGGGGCAAGAGACAAGTTTTGAGAGATCCCGGTTTTCTAGCAGGTCATCTTCCAAACAG AGTTCTAGTGAGGAGGATGCTCTAACTGAACCAAGGTCATCTATCAAGATATCTGCATTTAAGTGTGACT CCAAACTTCCAGCAATTGACCAAACATCAGTCAAGCAGAAACATAAAAGTACCATGACTGTAAGGAAAGC AGAGAAAGTGGACCCCAGCGAACCCTCTCCAGCAGAGTCAGAGCCGACTTGGTGGAATAAACCGGAAACA GACCTCGGAGGACCTTCTGGCTGGTGCAGTCTGGGGAAGAAAGGTCCCATGAGCAGAAGCATCCCTCCCT GTGAAGCAGACCAAGCACCAATGGTTTTATTAAGAAAGAGGAAGCCAAACCTGAGGAGATTTACAGTCAG CCCAGAATCGCACAGCCCGAGAGCATCTGGGGACAGAAGCAGACAGAAACAGCAGTGGCCTGCAAAGGTG CCGGTTCCCAGGGGAGCAGATCAAGTAGTTCAACAAGAAGGCCTGATGTGTAACACTAAGCTGAAGAGGC CAAATCAAGAGAGAAGAAACTTGGTCCCATCGTCACAGCCAATGACTGAGAACGCCCCTGATAGAGCCAA AAAAGGAGACCCGAGTGCTCCTTCCCAGAGTGAGCTGCATCCAGCCCTGTCCCAGGCCTTCCAAGGAAAA AATAGTCCTCAAGTATTGAGTGAGTTCTCGGGGCCACCACTCACACCCACCACTGTAGGAGGGCCAAGAA AGGCATCATTTAGGTTCCGAGATGAAGACTTTTATTCCATTTTGTCATTGAACAGCAGAAGAGAAAGCGA TGACACTGAAGAGGAAACCCAGTCTGAAGAATGTCTGTGGGTTGGTGTGCGCTCACCCTGTTCTCCTTCC CATCACAAAAGAAGTAGATTTGGGGGGACATCGACCCCTCAGGCCAAAAATAAAAATTTTGAAGAAAATG CTGAAAATTGCAGAGGTCATTCTTCAAGAAGAAGTGAGCCCAGTCATGGCTCATTGAGAATAAGCAATGC AATGGAGCCAGCAACAGAGCGGCCTTCTGCTGGGCAAAGGTTGTCCCAAGACCCCGGGCTGCCTGATAGG GAATCTGCTACAGAGAAGGACAGAGGTGGCAGTGAAAATGCGAAAAAGAGCCCTCTTTCGTGGGACACCA AATCCGAGCCCAGACAAGAGGTTGGTGTCAATGCTGAAAATGTATGGAGTGATTGTATTTCTGTGGAACA TAGGCCTGGCACCCATGATTCTGAAGGATACTGGAAAGACTATTTAAACAGTTCTCAAAATTCTCTTGAC TACTTTATTTCTGGCAGACCAATATCTCCAAGATCATCAGTGAATTCATCCTATAACCCTCCTGCATCAT TCATGCATTCTGCTCTGAGAGATGATATTCCAGTAGACTTGTCAATGTCATCGACTTCAGTTCACAGCTC AGACTCAGAGGGAAACTCAGGGTTTCATGTTTGCCAACCACTGTCTCCCATTAGAAATAGGACTCCATTT GCCAGTGCCGAAAACCATAATTATTTCCCAGTAAACAGTGCACACGAATTTGCTGTCAGGGAAGCAGAAG ATACTACTTTAACAAGCCAGCCTCAGGGGGCTCCACTATATACAGATCTCTTACTAAATCCACAGGGCAA TTTGTCCTTAGTGGATTCTTCCAGCTCCTCTCCATCAAGAATGAACTCAGAAGGCCATTTGCATGTGTCT GGGTCTCTGCAAGAAAATACACCATTTACTTTCTTTGCAGTGTCTCATTTCCCAAATCAAAATGATAATG GGAGCAGGATGGCAGCCTCTGGTTTCACAGATGAAAAGGAAACCAGTAAGATAAAGGCAGACCCTGAGAA ACTCAAGAAATTGCAAGAAAGTCTCCTGGAGGAGGACTCCGAGGAGGAGGGAGACTTGTGTCGCATCTGT CAGATAGCCGGGGGTTCCCCAAGCAACCCCCTCCTGGAGCCTTGCGGCTGTGTGGGAAGCCTGCAGTTTG TTCATCAAGAGTGCCTGAAAAAGTGGCTGAAAGTGAAAATAACATCAGGAGCAGATCTTGGTGCCGTGAA GACCTGTGAGATGTGTAAGCAAGGCCTGCTGGTTGACCTGGGTGACTTTAACATGATTGAGTTCTACCAG AAGCACCAGCAATCTCAGGCACAAAACGAGCTGATGAATTCAGGCTTGTACCTGGTGCTGCTGCTTCACC TCTATGAGCAGAGGTTTGCAGAACTCATGAGGCTCAACCACAACCAGGTGGAAAGAGAGAGGTTGTCAAG AAATTACCCACAACCCAGAACAGAGGAAAATGAAAATTCGGAGTTGGGAGATGGAAATGAAGGCAGCATT TCTCAAAGCCAGGTCGTCTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001288779
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001288779.1, NP_001275708.1
RefSeq Size	3268 bp
RefSeq ORF	2541 bp
Locus ID	162333
UniProt ID	Q8NA82
Gene Summary	MARCH10 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010] Transcript Variant: This variant (3) encodes the longest isoform (2).

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