NELL1 (NM_001288713) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IDH3GL; NRP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001288713, the custom clone sequence may differ by one or more nucleotides ATGCCGATGGATTTGATTTTAGTTGTGTGGTTCTGTGTGTGCACTGCCAGGACAGCAGAAGATATGAAGC CACCCGTGTTATCGCTGATATGGAGAACTAGAGTTCTGAAGTCTCTGCTTCAGCAATCCCTTCAGGGAGT GGTGGGCTTTGGGATGGACCCTGACCTTCAGATGGATATCGTCACCGAGCTTGACCTTGTGAACACCACC CTTGGAGTTGCTCAGGTGTCTGGAATGCACAATGCCAGCAAAGCATTTTTATTTCAAGACATAGAAAGAG AGATCCATGCAGCTCCTCATGTGAGTGAGAAATTAATTCAGCTGTTCCGGAACAAGAGTGAATTCACCAT TTTGGCCACTGTACAGCAGAAGCCATCCACTTCAGGAGTGATACTGTCCATTCGAGAACTGGAGCACAGC TATTTTGAACTGGAGAGCAGTGGCCTGAGGGATGAGATTCGGTATCACTACATACACAATGGGAAGCCAA GGACAGAGGCACTTCCTTACCGCATGGCAGATGGACAATGGCACAAGGTTGCACTGTCAGTTAGCGCCTC TCATCTCCTGCTCCATGTCGACTGTAACAGGATTTATGAGCGTGTGATAGACCCTCCAGATACCAACCTT CCCCCAGGAATCAATTTATGGCTTGGCCAGCGCAACCAAAAGCATGGCTTATTCAAAGGGATCATCCAAG ATGGGAAGATCATCTTTATGCCGAATGGATATATAACACAGTGTCCAAATCTAAATCACACTTGCCCAAC CTGCAGTGATTTCTTAAGCCTGGTGCAAGGAATAATGGATTTACAAGAGCTTTTGGCCAAGATGACTGCA AAACTAAATTATGCAGAGACAAGACTTAGTCAATTGGAAAACTGTCATTGTGAGAAGACTTGTCAAGTGA GTGGACTGCTCTATCGAGATCAAGACTCTTGGGTAGATGGTGACCATTGCAGGAACTGCACTTGCAAAAG TGGTGCCGTGGAATGCCGAAGGATGTCCTGTCCCCCTCTCAATTGCTCCCCAGACTCCCTCCCAGTGCAC ATTGCTGGCCAGTGCTGTAAGGTCTGCCGACCAAAATGTATCTATGGAGGAAAAGTTCTTGCAGAAGGCC AGCGGATTTTAACCAAGAGCTGTCGGGAATGCCGAGGTGGAGTTTTAGTAAAAATTACAGAAATGTGTCC TCCTTTGAACTGCTCAGAAAAGGATCACATTCTTCCTGAGAATCAGTGCTGCCGTGTCTGTAGAGGTCAT AACTTTTGTGCAGAAGGACCTAAATGTGGTGAAAACTCAGAGTGCAAAAACTGGAATACAAAAGCTACTT GTGAGTGCAAGAGTGGTTACATCTCTGTCCAGGGAGACTCTGCCTACTGTGAAGATATTGATGAGTGTGC AGCTAAGATGCATTACTGTCATGCCAATACTGTGTGTGTCAACCTTCCTGGGTTATATCGCTGTGACTGT GTCCCAGGATACATTCGTGTGGATGACTTCTCTTGTACAGAACACGATGAATGTGGCAGCGGCCAGCACA ACTGTGATGAGAATGCCATCTGCACCAACACTGTCCAGGGACACAGCTGCACCTGCAAACCGGGCTACGT GGGGAACGGGACCATCTGCAGAGCTTTCTGTGAAGAGGGCTGCAGATACGGTGGAACGTGTGTGGCTCCC AACAAATGTGTCTGTCCATCTGGATTCACAGGAAGCCACTGCGAGAAAGATATTGATGAATGTTCAGAGG GAATCATTGAGTGCCACAACCATTCCCGCTGCGTTAACCTGCCAGGGTGGTACCACTGTGAGTGCAGAAG CGGTTTCCATGACGATGGGACCTATTCACTGTCCGGGGAGTCCTGTATTGACATTGATGAATGTGCCTTA AGAACTCACACCTGTTGGAACGATTCTGCCTGCATCAACCTGGCAGGGGGCTTTGACTGTCTCTGCCCCT CTGGGCCCTCCTGCTCTGGTGACTGTCCTCATGAAGGGGGGCTGAAGCACAATGGCCAGGTGTGGACCTT GAAAGAAGACAGGTGTTCTGTCTGCTCCTGCAAGGATGGCAAGATATTCTGCCGACGGACAGCTTGTGAT TGCCAGAATCCAAGTGCTGACCTATTCTGTTGCCCAGAATGTGACACCAGAGTCACAAGTCAATGTTTAG ACCAAAATGGTCACAAGCTGTATCGAAGTGGAGACAATTGGACCCATAGCTGTCAGCAGTGTCGGTGTCT GGAAGGAGAGGTAGATTGCTGGCCACTCACTTGCCCCAACTTGAGCTGTGAGTATACAGCTATCTTAGAA GGGGAATGTTGTCCCCGCTGTGTCAGTGACCCCTGCCTAGCTGATAACATCACCTATGACATCAGAAAAA CTTGCCTGGACAGCTATGGTGTTTCACGGCTTAGTGGCTCAGTGTGGACGATGGCTGGATCTCCCTGCAC AACCTGTAAATGCAAGAATGGAAGAGTCTGTTGTTCTGTGGATTTTGAGTGTCTTCAAAATAATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001288713
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001288713.1, NP_001275642.1
RefSeq Size	3354 bp
RefSeq ORF	2517 bp
Locus ID	4745
UniProt ID	Q92832
Protein Families	Secreted Protein
Gene Summary	This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Transcript Variant: This variant (3) includes an alternate in-frame exon in the 5' coding region, compared to variant 1. The encoded isoform (3) is longer, compared to isoform 1.

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