CDC27 (NM_001293089) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ANAPC3; APC3; CDC27Hs; D0S1430E; D17S978E; H-NUC; HNUC; NUC2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001293089, the custom clone sequence may differ by one or more nucleotides ATGACGGTGCTGCAGGAACCCGTCCAGGCTGCTATATGGCAAGCACTAAACCACTATGCTTACCGAGATG CGGTTTTCCTCGCAGAACGCCTTTATGCAGAAGTACACTCAGAAGAAGCCTTGTTTTTACTGGCAACCTG TTATTACCGCTCAGGAAAGGCATATAAAGCATATAGACTCTTGAAAGGACACAGTTGTACTACACCGCAA TGCAAATACCTGCTTGCAAAATGTTGTGTTGATCTCAGCAAGCTTGCAGAAGGGGAACAAATCTTATCTG GTGGAGTGTTTAATAAGCAGAAAAGCCATGATGATATTGTTACTGAGTTTGGTGATTCAGCTTGCTTTAC TCTTTCATTGTTGGGACATGTATATTGCAAGACAGATCGGCTTGCCAAAGGATCAGAATGTTACCAAAAG AGCCTTAGTTTAAATCCTTTCCTCTGGTCTCCCTTTGAATCATTATGTGAAATAGGTGAAAAGCCAGATC CTGACCAAACATTTAAATTCACATCTTTACAGAACTTTAGCAACTGTCTGCCCAACTCTTGCACAACACA AGTACCTAATCATAGTTTATCTCACAGACAGCCTGAGACAGTTCTTACGGAAACACCCCAGGACACAATT GAATTAAACAGATTGAATTTAGAATCTTCCAATTCAAAGTACTCCTTGAATACAGATTCCTCAGTGTCTT ATATTGATTCAGCTGTAATTTCACCTGATACTGTCCCACTGGGAACAGGAACTTCCATATTATCTAAACA GGTTCAAAATAAACCAAAAACTGGTCGAAGTTTATTAGGAGGACCAGCAGCTCTTAGTCCATTAACCCCA AGTTTTGGGATTTTGCCATTAGAAACCCCAAGTCCTGGAGATGGATCCTATTTACAAAACTACACTAATA CACCTCCTGTAATTGATGTGCCATCCACCGGAGCCCCTTCAAAAAAGTCTGTTGCCAGAATCGGCCAAAC TGGAACAAAGTCTGTCTTCTCACAGAGTGGAAATAGCCGAGAGGTAACTCCAATTCTTGCACAAACACAA AGTTCTGGTCCACAAACAAGTACAACACCTCAGGTATTGAGCCCCACTATTACATCTCCCCCAAACGCAC TGCCTCGAAGAAGTTCACGACTCTTTACTAGTGACAGCTCCACAACCAAGGAGAATAGCAAAAAATTAAA AATGAAGTTTCCACCTAAAATCCCAAACAGAAAAACAAAAAGTAAAACTAATAAAGGAGGAATAACTCAA CCTAACATAAATGATAGCCTGGAAATTACAAAATTGGACTCTTCCATCATTTCAGAAGGGAAAATATCCA CAATCACACCTCAGATTCAGGCCTTTAATCTACAAAAAGCAGCAGCAGGTTTGATGAGCCTTCTTCGTGA AATGGGGAAAGGTTATTTAGCTTTGTGTTCATACAACTGCAAAGAAGCTATAAATATTTTGAGCCATCTA CCTTCTCACCACTACAATACTGGTTGGGTACTGTGCCAAATTGGAAGGGCCTATTTTGAACTTTCAGAGT ACATGCAAGCTGAAAGAATATTCTCAGAGGTTAGAAGGATTGAGAATTATAGAGTTGAAGGCATGGAGAT CTACTCTACAACACTTTGGCATCTTCAAAAAGATGTTGCTCTTTCAGTTCTGTCAAAAGACTTAACAGAC ATGGATAAAAATTCGCCAGAGGCCTGGTGTGCTGCAGGGAACTGTTTCAGTCTGCAACGGGAACATGATA TTGCAATTAAATTCTTCCAGAGAGCTATCCAAGTTGATCCAAATTACGCTTATGCCTATACTCTATTAGG GCATGAGTTTGTCTTAACTGAAGAATTGGACAAAGCATTAGCTTGTTTTCGAAATGCTATCAGAGTCAAT CCTAGACATTATAATGCATGGTATGGTTTAGGAATGATTTATTACAAGCAAGAAAAATTCAGCCTTGCAG AAATGCATTTCCAAAAAGCGCTTGATATCAACCCTCAAAGTTCAGTTTTACTTTGCCACATTGGAGTAGT TCAACATGCACTGAAAAAATCAGAGAAGGCTTTGGATACCCTAAACAAAGCCATTGTCATTGATCCCAAG AACCCTCTATGCAAATTTCACAGAGCCTCAGTTTTATTTGCAAATGAAAAATATAAGTCTGCTTTACAAG AACTTGAAGAATTGAAACAAATTGTTCCCAAAGAATCCCTCGTTTACTTCTTAATAGGAAAGGTTTACAA GAAGTTAGGTCAAACGCACCTCGCCCTGATGAATTTCTCTTGGGCTATGGATTTAGATCCTAAAGGAGCC AATAACCAGATTAAAGAGGCAATTGATAAGCGTTATCTTCCAGATGATGAGGAGCCAATAACCCAAGAAG AACAGATCATGGGAACAGATGAATCCCAGGAGAGCAGCATGACAGATGCGGATGACACACAACTTCATGC AGCTGAAAGTGATGAATTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293089
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293089.1, NP_001280018.1
RefSeq Size	5850 bp
RefSeq ORF	2472 bp
Locus ID	996
Protein Families	Druggable Genome
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
Gene Summary	The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014] Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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