Hsp105 (HSPH1) (NM_001286503) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HSP105; HSP105A; HSP105B; NY-CO-25
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286503, the custom clone sequence may differ by one or more nucleotides ATGTCGGTGGTGGGGTTGGACGTGGGCTCGCAGAGCTGCTACATCGCGGTAGCCCGGGCCGGGGGCATCG AGACCATCGCCAATGAGTTCAGCGACCGGTGCACCCCGTCAGTCATATCATTTGGATCAAAAAATAGAAC AATCGGAGTTGCAGCCAAAAATCAGCAAATCACTCATGCAAACAATACGGTGTCTAACTTCAAAAGATTT CATGGCCGAGCATTCAATGACCCCTTCATTCAAAAGGAGAAGGAAAACTTGAGTTACGATTTGGTTCCAT TGAAAAATGGTGGAGTTGGAATAAAGGTAATGTACATGGGTGAAGAACATCTATTTAGTGTGGAGCAGAT AACAGCCATGTTGTTGACTAAGCTGAAGGAAACTGCTGAAAACAGCCTCAAGAAACCAGTAACAGATTGT GTTATTTCAGTCCCCTCCTTCTTTACAGATGCTGAGAGGCGATCTGTGTTAGATGCTGCACAGATTGTTG GCCTAAACTGTTTAAGACTTATGAATGACATGACAGCTGTTGCTTTGAATTACGGAATTTATAAGCAGGA TCTCCCAAGCCTGGATGAGAAACCTCGGATAGTGGTTTTTGTTGATATGGGACATTCAGCTTTTCAAGTG TCTGCTTGTGCTTTTAACAAGGGAAAATTGAAGGTACTGGGAACAGCTTTTGATCCTTTCTTAGGAGGAA AAAACTTCGATGAAAAGTTAGTGGAACATTTTTGTGCAGAATTTAAAACTAAGTACAAGTTGGATGCAAA ATCCAAAATACGAGCACTCCTACGTCTGTATCAGGAATGTGAAAAACTGAAAAAGCTAATGAGCTCTAAC AGCACAGACCTTCCACTGAATATCGAATGCTTTATGAATGATAAAGATGTTTCCGGAAAGATGAACAGGT CACAATTTGAAGAACTCTGTGCTGAACTTCTGCAAAAGATAGAAGTACCCCTTTATTCACTGTTGGAACA AACTCATCTCAAAGTAGAAGATGTGAGTGCAGTTGAGATTGTTGGAGGCGCTACACGAATTCCAGCTGTG AAGGAAAGAATTGCCAAATTCTTTGGAAAAGATATTAGCACAACACTCAATGCAGATGAAGCAGTAGCCA GAGGATGTGCATTACAGTGTGCAATACTTTCCCCGGCATTTAAAGTTAGAGAATTTTCCGTCACAGATGC AGTTCCTTTTCCAATATCTCTGATCTGGAACCATGATTCAGAAGATACTGAAGGTGTTCATGAAGTCTTT AGTCGAAACCATGCTGCTCCTTTCTCCAAAGTTCTCACCTTTCTGAGAAGGGGGCCTTTTGAGCTAGAAG CTTTCTATTCTGATCCCCAAGGAGTTCCATATCCAGAAGCAAAAATAGGCCGCTTTGTAGTTCAGAATGT TTCTGCACAGAAAGATGGAGAAAAATCTAGAGTAAAAGTCAAAGTGCGAGTCAACACCCATGGCATTTTC ACCATCTCTACGGCATCTATGGTGGAGAAAGTCCCAACTGAGGAGAATGAAATGTCTTCTGAAGCTGACA TGGAGTGTCTGAATCAGAGACCACCAGAAAACCCAGACACTGATGCAAATGAAAAAAAAGTTGACCAGCC TCCAGAAGCTAAAAAGCCCAAAATAAAGGTGGTGAATGTTGAGCTGCCTATTGAAGCCAACTTGGTCTGG CAGTTAGGGAAAGACCTTCTTAACATGTATATTGAGACAGAGGGTAAGATGATAATGCAAGATAAATTGG AAAAAGAAAGGAATGATGCTAAAAATGCAGTTGAGGAATATGTGTATGAGTTCAGAGACAAGCTGTGTGG ACCATATGAAAAATTTATATGTGAGCAGGATCATCAAAATTTTTTGAGACTCCTCACAGAAACTGAAGAC TGGCTGTATGAAGAAGGAGAGGACCAAGCTAAACAAGCATATGTTGACAAGTTGGAAGAATTAATGAAAA TTGGCACTCCAGTTAAAGTTCGGTTTCAGGAAGCTGAAGAACGGCCAAAAATGTTTGAAGAACTAGGACA GAGGCTGCAGCATTATGCCAAGATAGCAGCTGACTTCAGAAATAAGGATGAGAAATACAACCATATTGAT GAGTCTGAAATGAAAAAAGTGGAGAAGTCTGTTAATGAAGTGATGGAATGGATGAATAATGTCATGAATG CTCAGGCTAAAAAGAGTCTTGATCAGGATCCAGTTGTACGTGCTCAGGAAATTAAAACAAAAATCAAGGA ATTGAACAACACATGTGAACCCGTTGTAACACAACCGAAACCAAAAATTGAATCACCCAAACTGGAAAGA ACTCCAAATGGCCCAAATATTGATAAAAAGGAAGAAGATTTAGAAGACAAAAACAATTTTGGTGCTGAAC CTCCACATCAGAATGGTGAATGTTACCCTAATGAGAAAAATTCTGTTAATATGGACTTGGACTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001286503
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286503.1, NP_001273432.1
RefSeq Size	5228 bp
RefSeq ORF	2445 bp
Locus ID	10808
UniProt ID	Q92598
Protein Families	Stem cell - Pluripotency
Gene Summary	This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2, also known as Hsp105beta) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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