SRGAP2 (NM_001300952) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARHGAP34; FNBP2; SRGAP2A; SRGAP3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300952, the custom clone sequence may differ by one or more nucleotides ATGACGTCTCCAGCCAAATTCAAAAAGGATAAGGAGATCATAGCAGAGTACGATACTCAGGTCAAAGAGA TCCGTGCTCAGCTCACAGAGCAGATGAAATGCCTGGACCAGCAGTGTGAGCTTCGGGTGCAACTGTTGCA GGACCTCCAGGACTTCTTCCGAAAGAAGGCAGAGATTGAGATGGACTACTCCCGCAACCTGGAGAAGCTG GCAGAACGCTTCCTGGCCAAGACACGCAGCACCAAGGACCAGCAATTCAAGAAGGATCAGAATGTTCTCT CTCCAGTCAACTGCTGGAATCTCCTCTTAAACCAGGTGAAGCGGGAAAGCAGGGACCATACCACCCTGAG TGACATCTACCTGAATAATATCATTCCTCGATTTGTACAAGTCAGCGAGGACTCAGGAAGACTCTTTAAA AAGAGTAAAGAAGTCGGCCAGCAGCTCCAAGATGATTTGATGAAGGTCCTGAACGAGCTCTACTCGGTGA TGAAGACATATCACATGTACAATGCCGACAGCATCAGTGCTCAGAGCAAACTAAAGGAGGCGGAGAAGCA GGAGGAGAAGCAAATTGGTAAATCGGTAAAGCAGGAGGACCGGCAGACCCCACGCTCCCCTGACTCCACG GCCAACGTTCGCATTGAGGAGAAACATGTCCGGAGGAGCTCAGTGAAGAAGATTGAGAAGATGAAGGAGA AGCGTCAAGCCAAGTACACGGAGAATAAGCTGAAGGCCATCAAAGCCCGGAATGAGTACTTGCTGGCTTT GGAGGCAACCAATGCATCTGTCTTCAAGTACTACATCCATGACCTATCTGACCTTATTGATTGTTGTGAC TTAGGCTACCATGCAAGTCTGAACCGGGCTCTACGCACCTTCCTCTCTGCTGAGTTAAACCTGGAACAGT CGAAGCATGAGGGTCTGGATGCCATCGAGAATGCAGTAGAAAACCTGGATGCCACCAGTGACAAGCAGCG CCTCATGGAGATGTACAACAACGTCTTCTGCCCCCCTATGAAGTTTGAGTTTCAGCCCCACATGGGGGAT ATGGCTTCCCAGCTCTGTGCCCAGCAGCCTGTCCAGAGTGAGCTGGTACAGAGATGCCAACAACTGCAGT CTCGCTTATCCACTCTAAAGATTGAAAACGAAGAGGTAAAGAAGACAATGGAGGCCACCCTGCAAACCAT CCAGGACATTGTGACTGTCGAGGACTTTGATGTGTCTGACTGCTTCCAGTACAGCAACTCCATGGAGTCC GTCAAGTCCACGGTCTCTGAAACCTTCATGAGCAAGCCCAGCATTGCTAAGAGGAGAGCCAACCAGCAAG AGACAGAGCAGTTTTATTTCACAAAAATGAAAGAGTACCTGGAGGGCAGGAACCTCATCACCAAGTTACA AGCCAAGCATGACCTTCTGCAGAAAACCCTGGGAGAAAGTCAGCGGACAGATTGCAGTCTAGCCAGGCGC AGCTCAACTGTGAGGAAACAGGACTCCAGCCAGGCAATTCCTCTGGTGGTGGAAAGCTGTATCCGGTTTA TCAGCAGACACGGACTACAGCATGAAGGAATTTTCCGGGTGTCAGGATCCCAGGTGGAAGTGAATGACAT CAAAAATGCCTTTGAGAGAGGAGAGGACCCCCTGGCTGGGGACCAGAACGACCATGACATGGATTCCATA GCTGGTGTCCTGAAGCTTTACTTCCGGGGGCTGGAACACCCTCTCTTCCCCAAGGACATCTTTCATGACC TGATGGCCTGCGTCACAATGGACAACCTGCAGGAGAGAGCTCTGCACATCCGGAAAGTCCTCCTAGTCCT GCCCAAAACCACTCTGATTATCATGAGATACCTCTTTGCCTTCCTCAATCATTTATCACAGTTCAGTGAA GAGAACATGATGGACCCCTACAACCTCGCCATCTGCTTCGGGCCCTCGCTAATGTCAGTGCCAGAGGGCC ACGACCAGGTGTCCTGCCAAGCCCACGTGAATGAGCTGATCAAAACCATCATCATCCAGCATGAGAACAT CTTCCCAAGCCCCAGGGAGCTGGAGGGCCCTGTCTACAGCAGAGGAGGAAGCATGGAGGATTACTGTGAT AGCCCTCATGGAGAGACTACCTCGGTTGAAGACTCAACCCAGGATGTGACCGCAGAGCACCACACGAGCG ATGACGAATGTGAGCCCATCGAGGCCATTGCCAAGTTTGACTACGTGGGCCGGACAGCCCGAGAGCTATC CTTTAAGAAGGGAGCATCCCTGCTGCTTTACCAGCGGGCTTCCGACGACTGGTGGGAAGGCCGGCACAAT GGCATCGACGGACTCATCCCCCATCAGTACATCGTGGTCCAAGACACGTTGATATCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001300952
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001300952.1, NP_001287881.1
RefSeq Size	4932 bp
RefSeq ORF	2370 bp
Locus ID	23380
Protein Pathways	Axon guidance
Gene Summary	This locus encodes a member of the SLIT-ROBO Rho GTPase activating protein family. The encoded protein stimulates GTPase activity of Rac1, and plays a role in cortical neuron development. This locus has several paralogs on human chromosome 1 resulting from segmental duplication. While this locus itself is conserved among various species, the paralogs are found only in the genus Homo, and not in the genomes of non-human great apes. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (4) uses an alternate in-frame splice site and differs in the 3' exon structure, result in a different 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (d) has a distinct C-terminus and is shorter than isoform a.

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