HIF1 beta (ARNT) (NM_001286035) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286035, the custom clone sequence may differ by one or more nucleotides ATGACATCAGATGTACCATCACTGGGTCCAGCCATTGCCTCTGGAAACTCTGGACCTGGAATTCAAGGTG GAGGAGCCATTGTCCAGAGGGCTATTAAGCGGCGACCAGGGCTGGATTTTGATGATGATGGAGAAGGGAA CAGTAAATTTTTGAGGTGTGATGATGATCAGATGTCTAACGATAAGGAGCGGTTTGCCAGGTCGGATGAT GAGCAGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATCACAGTGAAATTGAACGGCGGCGACGGA ACAAGATGACAGCCTACATCACAGAACTGTCAGATATGGTACCCACCTGTAGTGCCCTGGCTCGAAAACC AGACAAGCTAACCATCTTACGCATGGCAGTTTCTCACATGAAGTCCTTGCGGGGAACTGGCAACACATCC ACTGATGGCTCCTATAAGCCGTCTTTCCTCACTGATCAGGAACTGAAACATTTGATCTTGGAGGCAGCAG ATGGCTTTCTGTTTATTGTCTCATGTGAGACAGGCAGGGTGGTGTATGTGTCTGACTCCGTGACTCCTGT TTTGAACCAGCCACAGTCTGAATGGTTTGGCAGCACACTCTATGATCAGGTGCACCCAGATGATGTGGAT AAACTTCGTGAGCAGCTTTCCACTTCAGAAAATGCCCTGACAGGGCGTATCCTGGATCTAAAGACTGGAA CAGTGAAAAAGGAAGGTCAGCAGTCTTCCATGAGAATGTGTATGGGCTCAAGGAGATCGTTTATTTGCCG AATGAGGTGTGGCAGTAGCTCTGTGGACCCAGTTTCTGTGAATAGGCTGAGCTTTGTGAGGAACAGATGC AGGAATGGACTTGGCTCTGTAAAGGATGGGGAACCTCACTTCGTGGTGGTCCACTGCACAGGCTACATCA AGGCCTGGCCCCCAGCAGATGATGACCCAGAGGCTGGCCAGGGAAGCAAGTTTTGCCTAGTGGCCATTGG CAGATTGCAGGTAACTAGTTCTCCCAACTGTACAGACATGAGTAATGTTTGTCAACCAACAGAGTTCATC TCCCGACACAACATTGAGGGTATCTTCACTTTTGTGGATCACCGCTGTGTGGCTACTGTTGGCTACCAGC CACAGGAACTCTTAGGAAAGAATATTGTAGAATTCTGTCATCCTGAAGACCAGCAGCTTCTAAGAGACAG CTTCCAACAGGTAGTGAAATTAAAAGGCCAAGTGCTGTCTGTCATGTTCCGGTTCCGGTCTAAGAACCAA GAATGGCTCTGGATGAGAACCAGCTCCTTTACTTTCCAGAACCCTTACTCAGATGAAATTGAGTACATCA TCTGTACCAACACCAATGTGAAGAACTCTAGCCAAGAACCACGGCCTACACTCTCCAACACAATCCAGAG GCCACAACTAGGTCCCACAGCTAATTTACCCCTGGAGATGGGCTCAGGACAGCTGGCACCCAGGCAGCAG CAACAGCAAACAGAATTGGACATGGTACCAGGAAGAGATGGACTGGCCAGCTACAATCATTCCCAGGTGG TTCAGCCTGTGACAACCACAGGACCAGAACACAGCAAGCCCCTTGAGAAGTCAGATGGTTTATTTGCCCA GGATAGAGATCCAAGATTTTCAGAAATCTATCACAACATCAATGCGGATCAGAGTAAAGGCATCTCCTCC AGCACTGTCCCTGCCACCCAACAGCTATTCTCCCAGGGCAACACATTCCCTCCTACCCCCCGGCCGGCAG AGAATTTCAGGAATAGTGGCCTAGCCCCTCCTGTAACCATTGTCCAGCCATCAGCTTCTGCAGGACAGAT GTTGGCCCAGATTTCCCGCCACTCCAACCCCACCCAAGGAGCAACCCCAACTTGGACCCCTACTACCCGC TCAGGCTTTTCTGCCCAGCAGGTGGCTACCCAGGCTACTGCTAAGACTCGTACTTCCCAGTTTGGTGTGG GCAGCTTTCAGACTCCATCCTCCTTCAGCTCCATGTCCCTCCCTGGTGCCCCAACTGCATCGCCTGGTGC TGCTGCCTACCCTAGTCTCACCAATCGTGGATCTAACTTTGCTCCTGAGACTGGACAGACTGCAGGACAA TTCCAGACACGGACAGCAGAGGGTGTGGGTGTCTGGCCACAGTGGCAGGGCCAGCAGCCTCATCATCGTT CAAGTTCTAGTGAGCAACATGTTCAACAACCGCCAGCACAGCAACCTGGCCAGCCTGAGGTCTTCCAGGA GATGCTGTCCATGCTGGGAGATCAGAGCAACAGCTACAACAATGAAGAATTCCCTGATCTAACTATGTTT CCCCCCTTTTCAGAATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001286035
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286035.1, NP_001272964.1
RefSeq Size	4944 bp
RefSeq ORF	2328 bp
Locus ID	405
UniProt ID	P27540
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Pathways in cancer, Renal cell carcinoma
Gene Summary	This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] Transcript Variant: This variant (5) contains an alternate exon in the 5' region, initiates translation at a downstream in-frame start codon, and uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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