KA1 (GRIK4) (NM_001282473) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EAA1; GluK4; GluK4-2; GRIK; KA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282473, the custom clone sequence may differ by one or more nucleotides ATGCCCCGCGTCTCGGCGCCTTTGGTGCTGCTTCCTGCGTGGCTCGTGATGGTCGCCTGCAGCCCGCACT CCTTGAGGATCGCTGCTATCTTGGACGACCCCATGGAGTGCAGCAGAGGGGAGCGGCTCTCCATCACCCT GGCCAAGAACCGCATCAACCGCGCTCCTGAGAGGCTGGGCAAGGCCAAGGTCGAAGTGGACATCTTTGAG CTTCTCAGAGACAGCGAGTACGAGACTGCAGAAACCATGTGTCAGATCCTCCCCAAGGGGGTGGTCGCTG TCCTCGGACCATCGTCCAGCCCAGCCTCCAGCTCCATCATCAGCAACATCTGTGGAGAGAAGGAGGTCCC TCACTTCAAAGTGGCCCCAGAGGAGTTCGTCAAGTTCCAGTTCCAGAGATTCACAACCCTGAACCTCCAC CCCAGCAACACTGACATCAGCGTGGCTGTAGCTGGGATCCTGAACTTCTTCAACTGCACCACCGCCTGCC TCATCTGTGCCAAAGCAGAATGCCTTTTAAACCTAGAGAAGCTGCTCCGGCAATTCCTTATCTCCAAGGA CACGCTGTCCGTCCGCATGCTGGATGACACCCGGGACCCCACCCCGCTCCTCAAGGAGATCCGGGACGAC AAGACCGCCACCATCATCATCCACGCCAACGCCTCCATGTCCCACACCATCCTCCTGAAGGCAGCCGAAC TTGGGATGGTGTCAGCCTATTACACATACATCTTCACTAATCTGGAGTTCTCACTCCAGAGAATGGACAG CCTTGTGGATGATCGTGTCAACATCCTGGGATTTTCCATTTTCAACCAATCCCATGCTTTCTTCCAAGAG TTTGCCCAGAGCCTCAACCAGTCCTGGCAGGAGAACTGTGACCATGTGCCCTTCACTGGGCCTGCGCTCT CCTCGGCCCTGCTGTTTGATGCTGTCTATGCTGTGGTGACTGCGGTGCAGGAACTGAACCGGAGCCAAGA GATCGGCGTGAAGCCCTTGTCCTGCGGCTCGGCCCAGATCTGGCAGCACGGCACCAGCCTCATGAACTAC CTGCGCATGGTAGAATTGGAAGGTCTTACCGGCCACATTGAATTCAACAGCAAAGGCCAGAGGTCCAACT ACGCTTTGAAAATCTTACAGTTCACAAGGAATGGTTTTCGGCAGATCGGCCAGTGGCACGTGGCAGAGGG CCTCAGCATGGACAGCCACCTCTATGCCTCCAACATCTCGGACACTCTCTTCAACACCACCCTGGTCGTC ACCACCATCCTGGAGAACCCATATTTAATGCTGAAGGGGAACCACCAGGAGATGGAAGGCAATGACCGCT ACGAGGGCTTCTGTGTGGACATGCTCAAGGAGCTGGCAGAGATCCTCCGATTCAACTACAAGATCCGCCT GGTTGGGGATGGCGTGTACGGCGTTCCCGAGGCCAACGGCACCTGGACGGGAATGGTCGGGGAGCTGATC GCTAGGAAAGCAGATCTGGCTGTGGCAGGCCTCACCATTACAGCTGAACGGGAGAAGGTGATTGATTTCT CTAAGCCATTCATGACTCTGGGAATTAGCATTCTTTACCGCGTTCATATGGGACGCAAACCCGGCTATTT CTCCTTCCTGGACCCATTTTCTCCGGGCGTCTGGCTCTTCATGCTTCTAGCCTATCTGGCCGTCAGCTGT GTCCTCTTCCTGGTGGCTCGGTTGACGCCCTACGAGTGGTACAGCCCACACCCATGTGCCCAGGGCCGGT GCAACCTCCTGGTGAACCAGTACTCCCTGGGCAACAGCCTCTGGTTTCCGGTCGGGGGGTTCATGCAGCA AGGCTCCACCATCGCCCCTCGCGCCTTATCCACCCGCTGTGTCAGTGGCGTCTGGTGGGCATTCACGCTG ATCATCATCTCATCCTACACGGCCAACCTGGCAGCCTTCCTGACCGTGCAGCGCATGGATGTGCCCATTG AGTCAGTGGATGACCTGGCTGACCAGACCGCCATTGAATATGGCACAATTCACGGAGGCTCCAGCATGAC CTTCTTCCAAAATTCCCGCTACCAGACCTACCAACGCATGTGGAATTACATGTATTCCAAGCAGCCCAGC GTGTTCGTGAAGAGCACAGAGGAGGGAATCGCCAGGGTGTTGAATTCCAACTACGCCTTCCTCCTGGAAT CCACCATGAACGAGTACTATCGGCAGCGAAACTGCAACCTCACTCAGATTGGGGGCCTGCTGGACACCAA GGGCTATGGGATTGGCATGCCAGTCGGTATGCGGGAGAGGAACAGCCTCTTTGGGTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001282473
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282473.2, NP_001269402.1
RefSeq Size	3057 bp
RefSeq ORF	2298 bp
Locus ID	2900
Protein Families	Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
Gene Summary	This gene encodes a protein that belongs to the glutamate-gated ionic channel family. Glutamate functions as the major excitatory neurotransmitter in the central nervous system through activation of ligand-gated ion channels and G protein-coupled membrane receptors. The protein encoded by this gene forms functional heteromeric kainate-preferring ionic channels with the subunits encoded by related gene family members. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (3) has an additional exon in the 5' UTR but lacks several 3' terminal exons, compared to variant 1. Its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR. The resulting isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1.

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