KA1 (GRIK4) (NM_001282473) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	EAA1; GluK4; GluK4-2; GRIK; KA1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239521 representing NM_001282473 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCGTCTCGGCGCCTTTGGTGCTGCTTCCTGCGTGGCTCGTGATGGTCGCCTGCAGCCCGCACT CCTTGAGGATCGCTGCTATCTTGGACGACCCCATGGAGTGCAGCAGAGGGGAGCGGCTCTCCATCACCCT GGCCAAGAACCGCATCAACCGCGCTCCTGAGAGGCTGGGCAAGGCCAAGGTCGAAGTGGACATCTTTGAG CTTCTCAGAGACAGCGAGTACGAGACTGCAGAAACCATGTGTCAGATCCTCCCCAAGGGGGTGGTCGCTG TCCTCGGACCATCGTCCAGCCCAGCCTCCAGCTCCATCATCAGCAACATCTGTGGAGAGAAGGAGGTCCC TCACTTCAAAGTGGCCCCAGAGGAGTTCGTCAAGTTCCAGTTCCAGAGATTCACAACCCTGAACCTCCAC CCCAGCAACACTGACATCAGCGTGGCTGTAGCTGGGATCCTGAACTTCTTCAACTGCACCACCGCCTGCC TCATCTGTGCCAAAGCAGAATGCCTTTTAAACCTAGAGAAGCTGCTCCGGCAATTCCTTATCTCCAAGGA CACGCTGTCCGTCCGCATGCTGGATGACACCCGGGACCCCACCCCGCTCCTCAAGGAGATCCGGGACGAC AAGACCGCCACCATCATCATCCACGCCAACGCCTCCATGTCCCACACCATCCTCCTGAAGGCAGCCGAAC TTGGGATGGTGTCAGCCTATTACACATACATCTTCACTAATCTGGAGTTCTCACTCCAGAGAATGGACAG CCTTGTGGATGATCGTGTCAACATCCTGGGATTTTCCATTTTCAACCAATCCCATGCTTTCTTCCAAGAG TTTGCCCAGAGCCTCAACCAGTCCTGGCAGGAGAACTGTGACCATGTGCCCTTCACTGGGCCTGCGCTCT CCTCGGCCCTGCTGTTTGATGCTGTCTATGCTGTGGTGACTGCGGTGCAGGAACTGAACCGGAGCCAAGA GATCGGCGTGAAGCCCTTGTCCTGCGGCTCGGCCCAGATCTGGCAGCACGGCACCAGCCTCATGAACTAC CTGCGCATGGTAGAATTGGAAGGTCTTACCGGCCACATTGAATTCAACAGCAAAGGCCAGAGGTCCAACT ACGCTTTGAAAATCTTACAGTTCACAAGGAATGGTTTTCGGCAGATCGGCCAGTGGCACGTGGCAGAGGG CCTCAGCATGGACAGCCACCTCTATGCCTCCAACATCTCGGACACTCTCTTCAACACCACCCTGGTCGTC ACCACCATCCTGGAGAACCCATATTTAATGCTGAAGGGGAACCACCAGGAGATGGAAGGCAATGACCGCT ACGAGGGCTTCTGTGTGGACATGCTCAAGGAGCTGGCAGAGATCCTCCGATTCAACTACAAGATCCGCCT GGTTGGGGATGGCGTGTACGGCGTTCCCGAGGCCAACGGCACCTGGACGGGAATGGTCGGGGAGCTGATC GCTAGGAAAGCAGATCTGGCTGTGGCAGGCCTCACCATTACAGCTGAACGGGAGAAGGTGATTGATTTCT CTAAGCCATTCATGACTCTGGGAATTAGCATTCTTTACCGCGTTCATATGGGACGCAAACCCGGCTATTT CTCCTTCCTGGACCCATTTTCTCCGGGCGTCTGGCTCTTCATGCTTCTAGCCTATCTGGCCGTCAGCTGT GTCCTCTTCCTGGTGGCTCGGTTGACGCCCTACGAGTGGTACAGCCCACACCCATGTGCCCAGGGCCGGT GCAACCTCCTGGTGAACCAGTACTCCCTGGGCAACAGCCTCTGGTTTCCGGTCGGGGGGTTCATGCAGCA AGGCTCCACCATCGCCCCTCGCGCCTTATCCACCCGCTGTGTCAGTGGCGTCTGGTGGGCATTCACGCTG ATCATCATCTCATCCTACACGGCCAACCTGGCAGCCTTCCTGACCGTGCAGCGCATGGATGTGCCCATTG AGTCAGTGGATGACCTGGCTGACCAGACCGCCATTGAATATGGCACAATTCACGGAGGCTCCAGCATGAC CTTCTTCCAAAATTCCCGCTACCAGACCTACCAACGCATGTGGAATTACATGTATTCCAAGCAGCCCAGC GTGTTCGTGAAGAGCACAGAGGAGGGAATCGCCAGGGTGTTGAATTCCAACTACGCCTTCCTCCTGGAAT CCACCATGAACGAGTACTATCGGCAGCGAAACTGCAACCTCACTCAGATTGGGGGCCTGCTGGACACCAA GGGCTATGGGATTGGCATGCCAGTCGGTATGCGGGAGAGGAACAGCCTCTTTGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239521 representing NM_001282473 Red=Cloning site Green=Tags(s) MPRVSAPLVLLPAWLVMVACSPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFE LLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVAPEEFVKFQFQRFTTLNLH PSNTDISVAVAGILNFFNCTTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEIRDD KTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSLVDDRVNILGFSIFNQSHAFFQE FAQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNY LRMVELEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSHLYASNISDTLFNTTLVV TTILENPYLMLKGNHQEMEGNDRYEGFCVDMLKELAEILRFNYKIRLVGDGVYGVPEANGTWTGMVGELI ARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSC VLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQGSTIAPRALSTRCVSGVWWAFTL IIISSYTANLAAFLTVQRMDVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPS VFVKSTEEGIARVLNSNYAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGMRERNSLFG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282473
ORF Size	2295 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282473.2, NP_001269402.1
RefSeq Size	3057 bp
RefSeq ORF	2298 bp
Locus ID	2900
Protein Families	Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
Protein Pathways	Neuroactive ligand-receptor interaction
MW	86.2 kDa
Gene Summary	This gene encodes a protein that belongs to the glutamate-gated ionic channel family. Glutamate functions as the major excitatory neurotransmitter in the central nervous system through activation of ligand-gated ion channels and G protein-coupled membrane receptors. The protein encoded by this gene forms functional heteromeric kainate-preferring ionic channels with the subunits encoded by related gene family members. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]

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