MSK2 / RSK-B (RPS6KA4) (NM_001300802) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MSK2; RSK-B; S6K-alpha-4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001300802, the custom clone sequence may differ by one or more nucleotides ATGGGGGACGAGGACGACGATGAGAGCTGCGCCGTGGAGCTGCGGATCACAGAAGCCAACCTGACCGGGC ACGAGGAGAAGGTGAGCGTGGAGAACTTCGAGCTGCTCAAGGTGCTGGGCACGGGAGCCTACGGCAAGGT GTTCCTGGTGCGGAAGGCGGGCGGGCACGACGCGGGGAAGCTGTACGCCATGAAGGTGCTGCGCAAGGCG GCGCTGGTGCAGCGCGCCAAGACGCAAGAGCACACGCGCACCGAGCGCTCGGTGCTGGAGCTGGTGCGCC AGGCGCCCTTCCTGGTCACGCTGCACTACGCTTTCCAGACGGATGCCAAGCTGCACCTCATCCTGGACTA TGTGAGCGGCGGGGAGATGTTCACCCACCTCTACCAGCGCCAGTACTTCAAGGAGGCTGAGGTGCGCGTG TATGGGGGTGAGATCGTGCTGGCCCTGGAACACCTGCACAAGCTCGGCATCATTTACCGAGACCTGAAAC TGGAGAATGTGCTGCTGGACTCCGAGGGCCACATTGTCCTCACGGACTTCGGGCTGAGCAAGGAGTTCCT GACGGAGGAGAAAGAGCGGACCTTCTCCTTCTGTGGCACCATCGAGTACATGGCCCCCGAAATCATCCGT AGCAAGACGGGGCATGGCAAGGCTGTGGACTGGTGGAGCCTGGGCATCTTGCTCTTCGAGCTGCTGACGG GGGCCTCGCCCTTCACCCTGGAGGGCGAGAGGAACACGCAGGCTGAGGTGTCTCGACGGATCCTGAAGTG CTCCCCTCCCTTCCCCCCTCGGATCGGGCCCGTGGCGCAGGACCTGCTGCAGCGGCTGCTTTGTAAGGAT CCTAAGAAGCGATTGGGCGCGGGGCCCCAGGGGGCACAAGAAGTCCGGAACCATCCCTTCTTCCAGGGCC TCGATTGGGTGGCTCTGGCTGCCAGGAAGATTCCAGCCCCATTCCGGCCCCAAATCCGCTCAGAGCTGGA TGTGGGCAACTTTGCGGAGGAATTCACTCGGCTGGAGCCTGTCTACTCACCCCCTGGCAGCCCCCCACCT GGGGACCCCCGAATCTTTCAGGGATACTCCTTTGTGGCACCCTCCATTCTCTTTGACCACAACAACGCGG TGATGACCGATGGGCTGGAAGCGCCTGGTGCTGGAGACCGGCCAGGTCGGGCAGCGGTGGCCAGGAGCGC TATGATGCAGTACGAGCTGGACCTGCGGGAGCCTGCGCTGGGCCAGGGCAGCTTTTCTGTGTGTCGCCGC TGCCGCCAGCGCCAGAGCGGCCAGGAGTTCGCAGTCAAGATCCTCAGTCGCAGGCTGGAGGCGAACACGC AGCGCGAAGTGGCTGCCCTGCGCCTGTGCCAGTCACACCCCAACGTGGTGAATCTGCACGAGGTGCATCA CGACCAGCTGCACACGTACCTGGTCCTGGAGCTGCTGCGGGGCGGGGAGCTGCTGGAGCACATCCGCAAG AAGCGGCACTTCAGCGAGTCGGAAGCAAGCCAGATCCTGCGCAGCCTCGTGTCGGCCGTGAGCTTCATGC ACGAGGAGGCGGGCGTGGTGCACCGCGACCTCAAGCCGGAGAACATCCTGTACGCCGACGACACGCCCGG GGCCCCGGTGAAAATCATCGACTTCGGGTTCGCGCGGTTGCGGCCGCAGAGTCCCGGGGTGCCCATGCAG ACGCCCTGCTTCACGCTGCAGTACGCTGCCCCCGAGCTGCTGGCGCAGCAGGGCTACGACGAGTCCTGCG ACCTCTGGAGCCTGGGCGTCATTCTGTACATGATGCTGTCGGGGCAGGTCCCCTTCCAGGGGGCCTCTGG CCAGGGCGGGCAGAGCCAGGCGGCCGAGATCATGTGCAAAATCCGCGAGGGGCGCTTCTCCCTTGACGGG GAGGCCTGGCAGGGTGTATCCGAGGAAGCCAAGGAGCTGGTCCGAGGGCTCCTGACCGTGGACCCCGCCA AGCGGCTGAAGCTCGAGGGACTGCGGGGCAGCTCGTGGCTGCAGGACGGCAGCGCGCGCTCCTCGCCCCC GCTCCGGACGCCCGACGTGCTCGAGTCCTCTGGGCCCGCAGTGCGCTCGGGTCTCAACGCCACCTTCATG GCATTCAACCGGGGCAAGCGGGAGGGCTTCTTCCTGAAGAGCGTGGAGAATGCACCCCTGGCCAAGCGGC GGAAGCAGAAGCTGCGGAGCGCCACCGCCTCCCGCCGGGGCTCCCCTGCACCAGCCAACCCGGGCCGAGC CCCCGTCGCCTCCAAAGGGGCCCCCCGCCGAGCCAACGGCCCCCTGCCCCCCTCCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001300802
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001300802.1, NP_001287731.1
RefSeq Size	3128 bp
RefSeq ORF	2298 bp
Locus ID	8986
UniProt ID	O75676
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways	MAPK signaling pathway, Neurotrophin signaling pathway
Gene Summary	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform c).

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