GCOM1 (NM_001285900) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	gcom; Gcom2; GRINL1A; MYZAP; MYZAP-POLR2M
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001285900, the custom clone sequence may differ by one or more nucleotides ATGCTGCGCTCCACGTCCACGGTCACCCTGCTCTCGGGCGGCGCCGCCAGGACGCCCGGGGCGCCCAGCA GGAGGGCAAATGTTTGCAGACTACGGCTGACCGTACCTCCTGAGAGTCCAGTTCCTGAGCAATGTGAAAA GAAGATTGAGAGAAAAGAGCAGCTTCTTGACCTGAGCAATGGAGAACCTACCAGGAAACTTCCTCAGGGT GTTGTTTATGGTGTGGTGCGAAGATCAGATCAAAATCAGCAGAAAGAAATGGTGGTGTATGGGTGGTCCA CCAGTCAGCTGAAAGAAGAGATGAACTACATCAAAGATGTGAGAGCCACTTTGGAAAAGGTGAGAAAGCG AATGTATGGAGACTATGATGAGATGAGACAGAAGATTCGACAGCTCACCCAGGAACTATCAGTTTCCCAT GCTCAGCAGGAGTATCTGGAGAATCACATCCAAACCCAGTCGTCTGCCCTGGATCGTTTTAATGCCATGA ACTCAGCCTTGGCATCAGATTCCATTGGCCTGCAGAAAACCCTCGTGGATGTGACTTTGGAAAACAGCAA CATTAAGGATCAAATCAGAAATCTGCAGCAGACGTATGAAGCATCCATGGACAAGCTGAGGGAAAAGCAG AGGCAGTTGGAGGTAGCGCAAGTTGAAAACCAGCTGCTAAAAATGAAGGTGGAATCGTCCCAAGAAGCCA ATGCTGAGGTGATGCGAGAGATGACCAAGAAGCTGTACAGCCAGTATGAGGAGAAGCTGCAGGAAGAACA GAGGAAGCACAGTGCTGAGAAGGAGGCTCTTTTGGAAGAAACCAATAGTTTTCTGAAAGCGATTGAAGAA GCCAATAAAAAGATGCAAGCAGCAGAGATCAGCCTAGAGGAGAAAGACCAGAGGATCGGGGAGCTGGACA GGCTGATTGAGCGCATGGAAAAGGAACGTCATCAACTGCAACTTCAACTCCTAGAACATGAAACAGAAAT GTCTGGGGAGTTAACTGATTCTGACAAGGAAAGGTATCAGCAGTTGGAGGAGGCATCAGCCAGCCTCCGT GAGCGGATCAGACACCTAGATGACATGGTGCATTGCCAGCAGAAGAAAGTCAAGCAGATGGTCGAGGAGA TTGAATCATTAAAGAAAAAGTTGCAACAGAAACAGCTCTTAATACTGCAGCTTTTAGAAAAGATATCTTT CTTAGAAGGAGAGAATAATGAACTACAAAGCAGGTTGGACTATTTAACAGAAACCCAGGCCAAGACCGAA GTGGAAACCAGAGAGATAGGAGTGGGCTGTGATCTTCTACCCAGAAAATTCATTTGCAAATTGCCCGACA AAGGTAAAAAGATCTTTGACTCTTTTGCCAAACTGAAAGCTGCCATTGCAGAATGTGAAGAAGTTAGAAG AAAAAGTGAACTGTTTAACCCTGTTAGTTTAGACTGTAAGCTAAGGCAAAAAGCAATTGCAGAAGTTGAT GTGGGTACAGATAAGGCCCAGAATTCTGACCCGATACTTGATACTTCATCACTAGTTCCTGGATGTTCCT CTGTAGATAACATCAAGTCATCTCAAACCTCACAAAACCAGGGACTTGGACGTCCTACTCTTGAAGGTGA TGAAGAGACTTCAGAGGTTGAGTACACAGTGAATAAGGGCCCAGCTTCCAGCAATAGAGACAGGGTACCA CCTTCATCTGAAGCTAGTGAGCATCACCCGCGGCATCGTGTTTCAAGTCAAGCGGAAGATACTTCCAGCA GCTTTGACAACCTGTTTATTGACAGGTTACAGAGGATCACCATTGCGGACCAAGGTGAACAACAGTCAGA AGAAAACGCAAGTACTAAGAACTTGACAGGCCTTTCCAGTGGGACTGAGAAGAAACCTCATTACATGGAA GTGCTAGAAATGCGAGCCAAAAACCCAGTGCCCCAGCTGCGTAAATTTAAAACCAATGTGTTACCTTTTC GACAAAATGATTCATCTAGTCATTGCCAGAAGAGTGGGTCTCCTATTTCCTCAGAAGAGCGGCGGCGCAG GGATAAGCAGCATCTTGATGACATCACAGCAGCTCGGCTTCTACCACTTCACCATATGCCCACGCAGCTG CTCTCCATAGAAGAATCCTTGGCACTTCAGAAACAGCAGAAACAGAATTATGAGGAGATGCAAGCAAAGC TCGCAGCGCAAAAATTAGCTGAAAGACTGAATATTAAAATGCGGAGTTATAATCCAGAAGGGGAGTCTTC AGGGAGATACCGAGAAGTAAGGGATGAAGATGACGATTGGTCCTCTGATGAATTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001285900
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001285900.3, NP_001272829.1
RefSeq Size	5307 bp
RefSeq ORF	2298 bp
Locus ID	145781
Protein Families	Druggable Genome
Gene Summary	This locus represents naturally occurring readthrough transcription between the neighboring MYZAP (myocardial zonula adherens protein) and POLR2M (polymerase (RNA) II (DNA directed) polypeptide M) genes on chromosome 15. Alternative splicing results in multiple readthrough transcript variants. Readthrough variants may encode proteins that share sequence identity with the upstream gene product or with both the upstream and downstream gene products. Some readthrough transcript variants are also expected to be candidates for nonsense-mediated decay (NMD). [provided by RefSeq, Oct 2013] Transcript Variant: This variant (14, also known as Gcom15B) contains an alternate in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (3) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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