CSGLCAT (CHPF2) (NM_001284295) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	chPF-2; ChSy-3; CSGLCA-T; CSGlcAT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001284295, the custom clone sequence may differ by one or more nucleotides ATGCTGTCTCTGGCGCGGCCTCCGCTCCCGCCGACTGGCCTGAGAACGAGCCTGAGCCTCCTGCGGGTTT CCTGGATCCAGGGGGAGGGAGAAGATCCCTGTGTCGAGGCTGTAGGGGAGCGAGGAGGGCCACAGAATCC AGATTCCAGAGCTCGGCTAGACCAAAGTGATGAAGACTTCAAACCCCGGATTGTCCCCTACTACAGGGAC CCCAACAAGCCCTACAAGAAGGTGCTCAGGACTCGGTACATCCAGACAGAGCTGGGCTCCCGTGAGCGGT TGCTGGTGGCTGTCCTGACCTCCCGAGCTACACTGTCCACTTTGGCCGTGGCTGTGAACCGTACGGTGGC CCATCACTTCCCTCGGTTACTCTACTTCACTGGGCAGCGGGGGGCCCGGGCTCCAGCAGGGATGCAGGTG GTGTCTCATGGGGATGAGCGGCCCGCCTGGCTCATGTCAGAGACCCTGCGCCACCTTCACACACACTTTG GGGCCGACTACGACTGGTTCTTCATCATGCAGGATGACACATATGTGCAGGCCCCCCGCCTGGCAGCCCT TGCTGGCCACCTCAGCATCAACCAAGACCTGTACTTAGGCCGGGCAGAGGAGTTCATTGGCGCAGGCGAG CAGGCCCGGTACTGTCATGGGGGCTTTGGCTACCTGTTGTCACGGAGTCTCCTGCTTCGTCTGCGGCCAC ATCTGGATGGCTGCCGAGGAGACATTCTCAGTGCCCGTCCTGACGAGTGGCTTGGACGCTGCCTCATTGA CTCTCTGGGCGTCGGCTGTGTCTCACAGCACCAGGGGCAGCAGTATCGCTCATTTGAACTGGCCAAAAAT AGGGACCCTGAGAAGGAAGGGAGCTCGGCTTTCCTGAGTGCCTTCGCCGTGCACCCTGTCTCCGAAGGTA CCCTCATGTACCGGCTCCACAAACGCTTCAGCGCTCTGGAGTTGGAGCGGGCTTACAGTGAAATAGAACA ACTGCAGGCTCAGATCCGGAACCTGACCGTGCTGACCCCCGAAGGGGAGGCAGGGCTGAGCTGGCCCGTT GGGCTCCCTGCTCCTTTCACACCACACTCTCGCTTTGAGGTGCTGGGCTGGGACTACTTCACAGAGCAGC ACACCTTCTCCTGTGCAGATGGGGCTCCCAAGTGCCCACTACAGGGGGCTAGCAGGGCGGACGTGGGTGA TGCGTTGGAGACTGCCCTGGAGCAGCTCAATCGGCGCTATCAGCCCCGCCTGCGCTTCCAGAAGCAGCGA CTGCTCAACGGCTATCGGCGCTTCGACCCAGCACGGGGCATGGAGTACACCCTGGACCTGCTGTTGGAAT GTGTGACACAGCGTGGGCACCGGCGGGCCCTGGCTCGCAGGGTCAGCCTGCTGCGGCCACTGAGCCGGGT GGAAATCCTACCTATGCCCTATGTCACTGAGGCCACCCGAGTGCAGCTGGTGCTGCCACTCCTGGTGGCT GAAGCTGCTGCAGCCCCGGCTTTCCTCGAGGCCTTTGCAGCCAATGTCCTGGAGCCACGAGAACATGCAT TGCTCACCCTGTTGCTGGTCTACGGGCCACGAGAAGGTGGCCGTGGAGCTCCAGACCCATTTCTTGGGGT GAAGGCTGCAGCAGCGGAGTTAGAGCGACGGTACCCTGGGACGAGGCTGGCCTGGCTCGCTGTGCGAGCA GAGGCCCCTTCCCAGGTGCGACTCATGGACGTGGTCTCGAAGAAGCACCCTGTGGACACTCTCTTCTTCC TTACCACCGTGTGGACAAGGCCTGGGCCCGAAGTCCTCAACCGCTGTCGCATGAATGCCATCTCTGGCTG GCAGGCCTTCTTTCCAGTCCATTTCCAGGAGTTCAATCCTGCCCTGTCACCACAGAGATCACCCCCAGGG CCCCCGGGGGCTGGCCCTGACCCCCCCTCCCCTCCTGGTGCTGACCCCTCCCGGGGGGCTCCTATAGGGG GGAGATTTGACCGGCAGGCTTCTGCGGAGGGCTGCTTCTACAACGCTGACTACCTGGCGGCCCGAGCCCG GCTGGCAGGTGAACTGGCAGGCCAGGAAGAGGAGGAAGCCCTGGAGGGGCTGGAGGTGATGGATGTTTTC CTCCGGTTCTCAGGGCTCCACCTCTTTCGGGCCGTAGAGCCAGGGCTGGTGCAGAAGTTCTCCCTGCGAG ACTGCAGCCCACGGCTCAGTGAAGAACTCTACCACCGCTGCCGCCTCAGCAACCTGGAGGGGCTAGGGGG CCGTGCCCAGCTGGCTATGGCTCTCTTTGAGCAGGAGCAGGCCAATAGCACTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001284295
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001284295.1, NP_001271224.1
RefSeq Size	2730 bp
RefSeq ORF	2295 bp
Locus ID	54480
Protein Pathways	Chondroitin sulfate biosynthesis, Metabolic pathways
Gene Summary	Transfers glucuronic acid (GlcUA) from UDP-GlcUA to N-acetylgalactosamine residues on the non-reducing end of the elongating chondroitin polymer. Has no N-acetylgalactosaminyltransferase activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks a segment in the 5' UTR and 5' coding region which results in the use of an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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