TLE3 (NM_001282981) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ESG; ESG3; GRG3; HsT18976
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001282981, the custom clone sequence may differ by one or more nucleotides ATGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCTGAGTCTTGTGACAGGATCAAAG ACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTACGACAAGCTGGCAAACGAGAA GACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGCTTGAACATTGAAATGCACAAG CAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGCCTTTCCTGTCACAAGAGCACC AGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACCATGACGGAGCTGAACGCCATCATCGG GCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACACGGCCCCCCGGTCCAGTTGCCACCCCAC CCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGGAGCAGCTCCGGGCTGCTGGCACTGGGCG CCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCATGAACTCGATCACAGAGAGAG AGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCCAGTGAGAAGCACCGGGGCTCT GCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAGGAGAAGGACAGCTTGAGCCGATACGACAGTG ATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGAGGACCCCGCAACGCCCCGGGTCAGCCC GGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCCCGTAGCCTGAAAAAAGATGCCCCCACCAGCCCT GCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCCAAGACCAAAGACCTTGGTCATAACGACAAATCCT CCACCCCTGGGCTCAAGTCCAACACACCAACCCCAAGGAACGACGCCCCAACTCCAGGCACCAGCACGAC CCCAGGGCTCAGGTCGATGCCGGGTAAACCTCCGGGCATGGACCCGATAGCCTCGGCTCTGCGCACGCCC ATCTCCATCACCAGCTCCTATGCGGCGCCCTTCGCCATGATGAGCCACCATGAGATGAACGGCTCCCTCA CCAGTCCTGGCGCCTACGCCGGCCTCCACAACATCCCACCCCAGATGAGCGCCGCCGCCGCTGCTGCAGC CGCTGCCTATGGCCGATCGCCAATGGTGAGCTTTGGAGCTGTTGGTTTTGACCCTCACCCCCCGATGCGG GCCACAGGCCTCCCCTCAAGCCTGGCCTCCATTCCTGGAGGAAAACCAGCGTACTCATTCCATGTGAGTG CTGATGGGCAGATGCAGCCCGTGCCCTTCCCCCACGACGCCCTGGCAGGCCCCGGCATCCCGAGGCACGC CCGGCAGATCAACACACTCAGCCACGGGGAGGTGGTGTGTGCCGTGACCATCAGCAACCCCACGAGGCAC GTCTACACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACATCAGCCAGCCAGGCAGCAAGAGCCCCATCT CCCAGCTGGACTGCCTGAACAGGGACAATTACATCCGCTCCTGCAAGCTGCTCCCTGATGGGCGCACGCT CATCGTGGGCGGCGAGGCCAGCACGCTCACCATCTGGGACCTGGCCTCGCCCACGCCCCGCATCAAGGCC GAGCTGACGTCCTCGGCTCCCGCCTGTTATGCCCTGGCCATTAGCCCTGACGCCAAAGTCTGCTTCTCCT GCTGCAGCGATGGGAACATTGCTGTCTGGGACCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCA CACAGATGGGGCCAGCTGCATAGACATCTCCCATGATGGCACCAAACTGTGGACAGGGGGCCTGGACAAC ACGGTGCGCTCCTGGGACCTGCGGGAGGGCCGACAGCTACAGCAGCATGACTTCACTTCCCAGATCTTCT CGCTGGGCTACTGCCCCACTGGGGAGTGGCTGGCTGTGGGCATGGAGAGCAGCAACGTGGAGGTGCTGCA CCACACCAAGCCTGACAAGTACCAGCTGCACCTGCACGAGAGCTGCGTGCTCTCCCTCAAGTTCGCCTAC TGCGGCAAGTGGTTCGTGAGCACTGGGAAAGATAACCTTCTCAACGCCTGGAGGACGCCTTATGGAGCCA GCATATTCCAGTCTAAAGAATCCTCGTCTGTCTTGAGTTGTGACATTTCAGCGGATGACAAATACATTGT AACAGGCTCTGGTGACAAGAAGGCCACAGTTTATGAGGTCATCTACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282981
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282981.1, NP_001269910.1
RefSeq Size	4684 bp
RefSeq ORF	2289 bp
Locus ID	7090
UniProt ID	Q04726
Protein Families	Transcription Factors
Gene Summary	This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (6) has multiple differences compared to variant 1. These differences result in a novel 5' coding region and 5' UTR compared to variant 1. The encoded isoform (f) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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