TLE3 (NM_001282981) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ESG; ESG3; GRG3; HsT18976
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC239509 representing NM_001282981 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCCCCATCAACCCGGGCAGCCGGGATTTAAATTCACGGTGGCTGAGTCTTGTGACAGGATCAAAG ACGAATTCCAGTTCCTGCAAGCTCAGTATCACAGCCTCAAAGTGGAGTACGACAAGCTGGCAAACGAGAA GACGGAGATGCAGCGCCATTATGTGATGTACTATGAGATGTCCTATGGCTTGAACATTGAAATGCACAAG CAGACAGAGATTGCGAAGAGACTGAACACAATTTTAGCACAGATCATGCCTTTCCTGTCACAAGAGCACC AGCAGCAGGTGGCGCAGGCAGTGGAGCGCGCCAAGCAGGTCACCATGACGGAGCTGAACGCCATCATCGG GCAGCAGCAGCTCCAGGCGCAGCACCTCTCCCATGCCACACACGGCCCCCCGGTCCAGTTGCCACCCCAC CCGTCAGGTCTCCAGCCTCCAGGAATCCCCCCAGTGACAGGGAGCAGCTCCGGGCTGCTGGCACTGGGCG CCCTGGGCAGCCAGGCCCATCTGACGGTGAAGGATGAGAAGAACCACCATGAACTCGATCACAGAGAGAG AGAATCCAGTGCGAATAACTCTGTGTCACCCTCGGAAAGCCTCCGGGCCAGTGAGAAGCACCGGGGCTCT GCGGACTACAGCATGGAAGCCAAGAAGCGGAAGGCGGAGGAGAAGGACAGCTTGAGCCGATACGACAGTG ATGGAGACAAGAGTGATGATCTGGTGGTGGATGTTTCCAATGAGGACCCCGCAACGCCCCGGGTCAGCCC GGCACACTCCCCTCCTGAAAATGGGCTGGACAAGGCCCGTAGCCTGAAAAAAGATGCCCCCACCAGCCCT GCCTCGGTGGCCTCTTCCAGTAGCACACCTTCCTCCAAGACCAAAGACCTTGGTCATAACGACAAATCCT CCACCCCTGGGCTCAAGTCCAACACACCAACCCCAAGGAACGACGCCCCAACTCCAGGCACCAGCACGAC CCCAGGGCTCAGGTCGATGCCGGGTAAACCTCCGGGCATGGACCCGATAGCCTCGGCTCTGCGCACGCCC ATCTCCATCACCAGCTCCTATGCGGCGCCCTTCGCCATGATGAGCCACCATGAGATGAACGGCTCCCTCA CCAGTCCTGGCGCCTACGCCGGCCTCCACAACATCCCACCCCAGATGAGCGCCGCCGCCGCTGCTGCAGC CGCTGCCTATGGCCGATCGCCAATGGTGAGCTTTGGAGCTGTTGGTTTTGACCCTCACCCCCCGATGCGG GCCACAGGCCTCCCCTCAAGCCTGGCCTCCATTCCTGGAGGAAAACCAGCGTACTCATTCCATGTGAGTG CTGATGGGCAGATGCAGCCCGTGCCCTTCCCCCACGACGCCCTGGCAGGCCCCGGCATCCCGAGGCACGC CCGGCAGATCAACACACTCAGCCACGGGGAGGTGGTGTGTGCCGTGACCATCAGCAACCCCACGAGGCAC GTCTACACAGGTGGCAAGGGCTGCGTGAAGATCTGGGACATCAGCCAGCCAGGCAGCAAGAGCCCCATCT CCCAGCTGGACTGCCTGAACAGGGACAATTACATCCGCTCCTGCAAGCTGCTCCCTGATGGGCGCACGCT CATCGTGGGCGGCGAGGCCAGCACGCTCACCATCTGGGACCTGGCCTCGCCCACGCCCCGCATCAAGGCC GAGCTGACGTCCTCGGCTCCCGCCTGTTATGCCCTGGCCATTAGCCCTGACGCCAAAGTCTGCTTCTCCT GCTGCAGCGATGGGAACATTGCTGTCTGGGACCTGCACAACCAGACCCTGGTCAGGCAGTTCCAGGGCCA CACAGATGGGGCCAGCTGCATAGACATCTCCCATGATGGCACCAAACTGTGGACAGGGGGCCTGGACAAC ACGGTGCGCTCCTGGGACCTGCGGGAGGGCCGACAGCTACAGCAGCATGACTTCACTTCCCAGATCTTCT CGCTGGGCTACTGCCCCACTGGGGAGTGGCTGGCTGTGGGCATGGAGAGCAGCAACGTGGAGGTGCTGCA CCACACCAAGCCTGACAAGTACCAGCTGCACCTGCACGAGAGCTGCGTGCTCTCCCTCAAGTTCGCCTAC TGCGGCAAGTGGTTCGTGAGCACTGGGAAAGATAACCTTCTCAACGCCTGGAGGACGCCTTATGGAGCCA GCATATTCCAGTCTAAAGAATCCTCGTCTGTCTTGAGTTGTGACATTTCAGCGGATGACAAATACATTGT AACAGGCTCTGGTGACAAGAAGGCCACAGTTTATGAGGTCATCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC239509 representing NM_001282981 Red=Cloning site Green=Tags(s) MAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHK QTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGPPVQLPPH PSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGS ADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLKKDAPTSP ASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPIASALRTP ISITSSYAAPFAMMSHHEMNGSLTSPGAYAGLHNIPPQMSAAAAAAAAAYGRSPMVSFGAVGFDPHPPMR ATGLPSSLASIPGGKPAYSFHVSADGQMQPVPFPHDALAGPGIPRHARQINTLSHGEVVCAVTISNPTRH VYTGGKGCVKIWDISQPGSKSPISQLDCLNRDNYIRSCKLLPDGRTLIVGGEASTLTIWDLASPTPRIKA ELTSSAPACYALAISPDAKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISHDGTKLWTGGLDN TVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHHTKPDKYQLHLHESCVLSLKFAY CGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISADDKYIVTGSGDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001282981
ORF Size	2286 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282981.1, NP_001269910.1
RefSeq Size	4684 bp
RefSeq ORF	2289 bp
Locus ID	7090
UniProt ID	Q04726
Protein Families	Transcription Factors
MW	82.7 kDa
Gene Summary	This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

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