DPP6 (NM_001290252) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DPL1; DPPX; MRD33; VF2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290252, the custom clone sequence may differ by one or more nucleotides ATGACCACAGCCAAGGAGCCAAGCGCTTCGGGGAAATCCGTGCAGCAGCAGGAACAGGAGCTGGTGGGGA GTAACCCTCCGCAGAGGAATTGGAAAGGAATAGCAATTGCACTGCTTGTCATTCTGGTCATCTGCTCCTT GATCGTCACCTCGGTCATACTTCTGACACCAGCGGAAGATAATAGTCTGTCTCAAAAGAAGAAGGTCACT GTAGAAGATCTCTTCAGTGAAGACTTCAAAATTCATGACCCCGAGGCTAAGTGGATAAGTGATACAGAAT TCATCTACAGAGAACAGAAAGGAACAGTGAGACTGTGGAATGTTGAAACAAATACTTCTACTGTCTTAAT AGAAGGCAAAAAAATTGAATCATTAAGAGCCATCAGATATGAAATATCTCCAGATAGAGAGTATGCACTT TTTTCATACAATGTGGAACCCATATTTATTTTTGAAAACAATATCTACTACTGTGCACATGTCGGGAAAC AGGCCATCCGTGTGGTCTCCACTGGCAAGGAAGGTGTGATTTACAATGGCCTCAGTGACTGGCTGTATGA AGAGGAGATTTTGAAGACACACATCGCACACTGGTGGTCTCCGGATGGCACGAGACTCGCCTACGCCGCC ATCAATGATTCCCGTGTCCCCATCATGGAGCTCCCAACTTACACCGGCTCCATCTACCCCACCGTGAAGC CCTACCACTATCCCAAGGCTGGAAGTGAGAACCCCAGCATTTCCCTACACGTTATTGGCTTAAATGGACC CACCCATGATCTGGAGATGATGCCGCCTGATGATCCACGGATGAGGGAGTACTACATCACCATGGTGAAG TGGGCCACCAGCACCAAGGTCGCCGTGACCTGGCTGAACCGGGCGCAGAACGTGTCCATCCTCACCCTCT GCGACGCCACCACGGGGGTCTGCACGAAGAAACACGAGGATGAAAGTGAGGCCTGGCTCCACAGACAGAA TGAAGAACCTGTGTTCTCCAAGGATGGCCGAAAGTTTTTCTTCATCAGAGCCATCCCCCAGGGAGGACGA GGGAAATTCTATCACATCACGGTGTCCTCGTCCCAGCCCAACAGCAGCAACGACAACATCCAGTCCATCA CCTCCGGGGACTGGGACGTGACCAAGATCCTAGCCTACGATGAGAAGGGGAATAAGATCTACTTCCTGAG CACGGAGGACCTGCCTCGGAGACGACAACTCTACAGTGCCAACACGGTGGGCAACTTCAACAGGCAGTGC CTCTCCTGTGACCTGGTTGAGAACTGCACCTACTTCAGCGCTTCCTTCAGCCATAGCATGGACTTCTTCC TGCTCAAGTGCGAAGGTCCTGGTGTTCCTATGGTGACGGTGCACAACACAACAGATAAGAAAAAAATGTT TGACCTAGAAACAAATGAACATGTCAAGAAGGCCATAAATGACCGACAGATGCCTAAAGTGGAATACAGG GACATTGAGATTGATGATTACAACCTGCCCATGCAGATACTGAAGCCAGCAACCTTCACCGACACCACCC ACTACCCTCTGCTCCTGGTGGTGGATGGCACCCCAGGCAGCCAGAGTGTGGCTGAGAAGTTCGAGGTGAG CTGGGAGACGGTGATGGTGAGCAGCCACGGCGCGGTGGTGGTAAAGTGTGACGGCCGTGGCAGCGGCTTC CAAGGGACCAAGCTCCTGCACGAAGTGAGGCGGCGGCTGGGCTTGCTGGAGGAGAAGGACCAGATGGAGG CCGTGCGGACGATGCTGAAGGAGCAGTACATTGACAGGACGCGCGTGGCCGTGTTTGGGAAGGATTACGG TGGCTACCTGAGCACCTACATCCTCCCAGCAAAGGGAGAAAATCAAGGCCAGACATTCACCTGCGGCTCT GCTCTCTCTCCAATAACAGACTTCAAACTCTATGCCTCTGCGTTTTCCGAGAGGTACTTGGGCCTCCATG GACTTGACAACAGAGCATACGAGATGACCAAGGTAGCCCATCGAGTCTCCGCGCTGGAAGAACAGCAGTT CCTGATCATTCATCCCACTGCCGATGAAAAAATTCATTTCCAGCACACAGCAGAACTCATTACACAACTA ATTAGGGGAAAGGCTAATTACAGCTTACAGATTTACCCGGACGAAAGCCATTACTTTACCAGCTCCAGCC TCAAACAGCATCTGTACCGGTCCATCATCAACTTCTTCGTGGAATGCTTCAGGATCCAGGACAAACTGCT GACAGTCACAGCGAAAGAGGACGAGGAGGAGGACTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290252
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290252.1, NP_001277181.1
RefSeq Size	4370 bp
RefSeq ORF	2277 bp
Locus ID	1804
Protein Families	Druggable Genome, Protease, Transmembrane
Gene Summary	This gene encodes a single-pass type II membrane protein that is a member of the peptidase S9B family of serine proteases. This protein has no detectable protease activity, most likely due to the absence of the conserved serine residue normally present in the catalytic domain of serine proteases. However, it does bind specific voltage-gated potassium channels and alters their expression and biophysical properties. Variations in this gene may be associated with susceptibility to amyotrophic lateral sclerosis and with idiopathic ventricular fibrillation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] Transcript Variant: This variant (4) contains an alternate 5'-most exon, initiates translation at an alternate start codon, and lacks two exons in the central coding region, compared to variant 1. The encoded isoform (4) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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